ID   TRM56_PICTO             Reviewed;         341 AA.
AC   Q6L171;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=tRNA (cytidine(56)-2'-O)-methyltransferase;
DE            EC=2.1.1.206;
DE   AltName: Full=tRNA ribose 2'-O-methyltransferase aTrm56;
GN   OrderedLocusNames=PTO0696;
OS   Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC
OS   100828).
OC   Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC   Picrophilaceae; Picrophilus.
OX   NCBI_TaxID=263820;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828;
RX   PubMed=15184674; DOI=10.1073/pnas.0401356101;
RA   Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C.,
RA   Schepers B., Dock C., Antranikian G., Liebl W.;
RT   "Genome sequence of Picrophilus torridus and its implications for life
RT   around pH 0.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004).
CC   -!- FUNCTION: Specifically catalyzes the AdoMet-dependent 2'-O-ribose
CC       methylation of cytidine at position 56 in tRNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-
CC         methylcytidine(56) in tRNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42968, Rhea:RHEA-COMP:10308, Rhea:RHEA-COMP:10309,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74495, ChEBI:CHEBI:82748; EC=2.1.1.206;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the aTrm56 family. {ECO:0000305}.
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DR   EMBL; AE017261; AAT43281.1; -; Genomic_DNA.
DR   RefSeq; WP_011177497.1; NC_005877.1.
DR   AlphaFoldDB; Q6L171; -.
DR   SMR; Q6L171; -.
DR   STRING; 263820.PTO0696; -.
DR   EnsemblBacteria; AAT43281; AAT43281; PTO0696.
DR   GeneID; 2844364; -.
DR   KEGG; pto:PTO0696; -.
DR   eggNOG; arCOG01857; Archaea.
DR   HOGENOM; CLU_817897_0_0_2; -.
DR   OMA; LRINHRP; -.
DR   OrthoDB; 61735at2157; -.
DR   Proteomes; UP000000438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008175; F:tRNA methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0002128; P:tRNA nucleoside ribose methylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00077; HDc; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   HAMAP; MF_00077; tRNA_methyltr_aTrm56; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   InterPro; IPR006675; HDIG_dom.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   InterPro; IPR002845; tRNA_mtfrase_aTrm56.
DR   PANTHER; PTHR42197; PTHR42197; 1.
DR   Pfam; PF01966; HD; 1.
DR   Pfam; PF01994; Trm56; 1.
DR   SMART; SM00471; HDc; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00277; HDIG; 1.
DR   PROSITE; PS51831; HD; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..341
FT                   /note="tRNA (cytidine(56)-2'-O)-methyltransferase"
FT                   /id="PRO_0000365325"
FT   DOMAIN          187..294
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
FT   BINDING         79
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         104..108
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   341 AA;  38517 MW;  D6662D3D7FCCFBDB CRC64;
     MITVLRIGHR PFRDKRITTH VALVARAFGA SSIYIDTRDD ELENTVKKVV DNFGGSFEVK
     TGIEWKSFMK KFHGTKVNLT MYGEPIEKRI DEIKSGDDIL VLVGAEKVPI DAYLIADYNI
     SVTNQPHSEV AALAIFLDRY FDGKELEKSY EGKINVVPMN HGKLVKYIPD EDQCLMILKS
     ENADDLIIRH VETVYKVAMR MADCIPCDRR LVAAGALLHD IGRTKTNNID HAIAGAEILK
     KKNIDDRIVR IVERHTGAGI TSEEAQKLGL PVKDYVPETI EEKIVAHADN LVSMDRIINL
     KQLMDKYENK NLHDAALRIK KLHEELSSIC GRDLDDITKD L