ID   TRM56_THEAC             Reviewed;         339 AA.
AC   Q9HJN6;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=tRNA (cytidine(56)-2'-O)-methyltransferase;
DE            EC=2.1.1.206;
DE   AltName: Full=tRNA ribose 2'-O-methyltransferase aTrm56;
GN   OrderedLocusNames=Ta0931;
OS   Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC
OS   15155 / AMRC-C165).
OC   Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC   Thermoplasmataceae; Thermoplasma.
OX   NCBI_TaxID=273075;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165;
RX   PubMed=11029001; DOI=10.1038/35035069;
RA   Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C.,
RA   Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.;
RT   "The genome sequence of the thermoacidophilic scavenger Thermoplasma
RT   acidophilum.";
RL   Nature 407:508-513(2000).
CC   -!- FUNCTION: Specifically catalyzes the AdoMet-dependent 2'-O-ribose
CC       methylation of cytidine at position 56 in tRNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-
CC         methylcytidine(56) in tRNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42968, Rhea:RHEA-COMP:10308, Rhea:RHEA-COMP:10309,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74495, ChEBI:CHEBI:82748; EC=2.1.1.206;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the aTrm56 family. {ECO:0000305}.
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DR   EMBL; AL445066; CAC12060.1; -; Genomic_DNA.
DR   RefSeq; WP_010901340.1; NC_002578.1.
DR   AlphaFoldDB; Q9HJN6; -.
DR   SMR; Q9HJN6; -.
DR   STRING; 273075.Ta0931; -.
DR   EnsemblBacteria; CAC12060; CAC12060; CAC12060.
DR   GeneID; 1456466; -.
DR   KEGG; tac:Ta0931; -.
DR   eggNOG; arCOG01857; Archaea.
DR   HOGENOM; CLU_817897_0_0_2; -.
DR   OMA; LRINHRP; -.
DR   OrthoDB; 61735at2157; -.
DR   BRENDA; 2.1.1.206; 6324.
DR   Proteomes; UP000001024; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008175; F:tRNA methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0002128; P:tRNA nucleoside ribose methylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00077; HDc; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   HAMAP; MF_00077; tRNA_methyltr_aTrm56; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   InterPro; IPR006675; HDIG_dom.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   InterPro; IPR002845; tRNA_mtfrase_aTrm56.
DR   PANTHER; PTHR42197; PTHR42197; 1.
DR   Pfam; PF01966; HD; 1.
DR   Pfam; PF01994; Trm56; 1.
DR   SMART; SM00471; HDc; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00277; HDIG; 1.
DR   PROSITE; PS51831; HD; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..339
FT                   /note="tRNA (cytidine(56)-2'-O)-methyltransferase"
FT                   /id="PRO_0000365326"
FT   DOMAIN          188..295
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
FT   BINDING         79
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         105..109
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   339 AA;  38120 MW;  74A0FB868268138A CRC64;
     MITVLRINHR PYRDKRITTH VALTARAFGA SAILVDERDE TLENTIRGVI SNFGGSFSIK
     TGCNWIQEFK HFQGIRVHLT MYGRRINDVI DEIRNSGKDV MVLVGSEKVP IEAYEIADYN
     VSVTNQPISE VSALAIFLDR YFQGKEFEFE FRGRINVQPA ERGKIVKIIP DEIECLDLLK
     KYGASEQLIE HVKAVEGLAL KIAERCNADK RVIVAGSLLH DIGRTRTNGI DHAVAGAEIL
     RSENIHDSVV SAVERHIGAG ITREEAARLG LPEKDYVPET LEEMIVAQAD NLFAGNKRLR
     LEEVLNIYRK RGLDSAAERI KELHRRISAI AGIDIDEIR