ID   TRM82_ASPCL             Reviewed;         495 AA.
AC   A1CRF7;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit trm82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=trm82; ORFNames=ACLA_029580;
OS   Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 /
OS   NRRL 1 / QM 1276 / 107).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=344612;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit trm8.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; DS027059; EAW08228.1; -; Genomic_DNA.
DR   RefSeq; XP_001269654.1; XM_001269653.1.
DR   AlphaFoldDB; A1CRF7; -.
DR   SMR; A1CRF7; -.
DR   STRING; 5057.CADACLAP00002018; -.
DR   EnsemblFungi; EAW08228; EAW08228; ACLA_029580.
DR   GeneID; 4701535; -.
DR   KEGG; act:ACLA_029580; -.
DR   VEuPathDB; FungiDB:ACLA_029580; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_022082_0_0_1; -.
DR   OMA; WVFEHDG; -.
DR   OrthoDB; 937275at2759; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000006701; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 2.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..495
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit trm82"
FT                   /id="PRO_0000370512"
FT   REPEAT          99..139
FT                   /note="WD 1"
FT   REPEAT          245..290
FT                   /note="WD 2"
FT   REPEAT          295..337
FT                   /note="WD 3"
FT   REGION          51..100
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        64..91
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   495 AA;  54140 MW;  F98D4BE28ABF3E92 CRC64;
     MAATFQYPFQ CIKSVERRNS GRQLIIGSAG PKIYTYAAET GERLGIWPET ANTAEAKAAP
     STTGEEPPEK RRKVSPPPDQ KPEDSEPASQ KSRKPEASPA WSTIPILAVS ADGEYVVAVT
     AEDKCIRVFE VEENGALKQL SERQMPKRPS AIALADDDRT ILCGDKFGDV YSLPLIDTGK
     GSVAPRAPAK VRPDQPAATT LTVHSKRNLA SLEQQLRHYG QKDKNSAEEK PSSTFEHHLL
     LGHVSMLTDL ICVSIPMDSS SEKKRSYILT ADRDEHIRVS RGPSQAHIIE NYCLGHTSFV
     NSLCIPQWAP EYLVSGGGDN YLLVWRWNEG RIVQKVPLVD ETSDSEVAVR GIWVTDKMVL
     VAIDGSAKLL CFTLESDGTM KAQNSIQASG NVLDLTISSK DSAVLVSVDA VHVAGSTQEW
     RATPSSSSTL IEAFRLKSDT ENVEWEPMSE AITSQVNSQG TSEISATLEE KQKKELNDAL
     YSSGNLRKKN LGEDE