ID   TRM82_ASPOR             Reviewed;         493 AA.
AC   Q2UKH7;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit trm82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=trm82; ORFNames=AO090003000804;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit trm8.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; AP007155; BAE57938.1; -; Genomic_DNA.
DR   RefSeq; XP_001819940.1; XM_001819888.1.
DR   AlphaFoldDB; Q2UKH7; -.
DR   SMR; Q2UKH7; -.
DR   STRING; 510516.Q2UKH7; -.
DR   EnsemblFungi; BAE57938; BAE57938; AO090003000804.
DR   GeneID; 5991923; -.
DR   KEGG; aor:AO090003000804; -.
DR   VEuPathDB; FungiDB:AO090003000804; -.
DR   HOGENOM; CLU_022082_0_0_1; -.
DR   OMA; WVFEHDG; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000006564; Chromosome 2.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..493
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit trm82"
FT                   /id="PRO_0000370515"
FT   REPEAT          99..139
FT                   /note="WD 1"
FT   REPEAT          237..287
FT                   /note="WD 2"
FT   REPEAT          289..331
FT                   /note="WD 3"
FT   REGION          46..96
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        46..69
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        70..86
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   493 AA;  54112 MW;  4C96F115F4034EA9 CRC64;
     MAANFQLPLQ CLQYLEKRGA ESQRFLIASS GGKIYSYAAE TGQRLSSWPQ DVDASNANNS
     KATETETGSE DQAPPEKKRK VSPSEEGPAE TSKSTVKAST WSSIPCLVAH SNGDYVIALT
     AEDKCVRVLR LKDDGTLEQL SERCMPKRPC SIALTDDGNT ILCGDKFGDV YSLPLLPGNE
     PYVAPKLPNR PKVPSATPLT VHSKRNLESL EQQLRYSQKN STEEKNSLNF QHQLLLGHVS
     LLTDVAFVTV PQDDNFGKKR SYILTGDRDE HIRVSRYPQA HIIEGYCLGH TAFVTKLCIP
     QYAPGYLISG GGDDYLLVWK WSEGRILQKV PLVKQESETT QVTVRGIWAT SIGGSNIVLV
     ALEGSSNLQC FVLGSDGTLK PQDPIEMSGN VLDVAIMEKD STIVVSVDCI REKGSTHEWR
     ASPTSPSNLI ESFRVKPGTE NLEWEPVTES LVTNINMGGS SGIPADADTK QRKELNDVLY
     SLGNLRKKHG EDD