ID   TRM82_COCIM             Reviewed;         520 AA.
AC   Q1DXS8; J3KEY4;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=TRM82 {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=CIMG_04885;
OS   Coccidioides immitis (strain RS) (Valley fever fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX   NCBI_TaxID=246410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RS;
RX   PubMed=19717792; DOI=10.1101/gr.087551.108;
RA   Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA   Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA   McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA   Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA   Henn M.R., Birren B.W., Taylor J.W.;
RT   "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT   and their relatives.";
RL   Genome Res. 19:1722-1731(2009).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=RS;
RX   PubMed=20516208; DOI=10.1101/gr.103911.109;
RA   Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J.,
RA   Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D.,
RA   Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A.,
RA   FitzGerald M., Lui A., Macdonald J.P., Priest M., Orbach M.J.,
RA   Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R.,
RA   Taylor J.W., Rounsley S.D.;
RT   "Population genomic sequencing of Coccidioides fungi reveals recent
RT   hybridization and transposon control.";
RL   Genome Res. 20:938-946(2010).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit TRM8.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; GG704914; EAS33861.3; -; Genomic_DNA.
DR   RefSeq; XP_001245444.1; XM_001245443.2.
DR   AlphaFoldDB; Q1DXS8; -.
DR   STRING; 246410.Q1DXS8; -.
DR   EnsemblFungi; EAS33861; EAS33861; CIMG_04885.
DR   GeneID; 4563280; -.
DR   KEGG; cim:CIMG_04885; -.
DR   VEuPathDB; FungiDB:CIMG_04885; -.
DR   InParanoid; Q1DXS8; -.
DR   OMA; WVFEHDG; -.
DR   OrthoDB; 937275at2759; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000001261; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..520
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit TRM82"
FT                   /id="PRO_0000370518"
FT   REPEAT          105..145
FT                   /note="WD 1"
FT   REPEAT          244..291
FT                   /note="WD 2"
FT   REPEAT          296..338
FT                   /note="WD 3"
FT   REGION          51..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        84..102
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   520 AA;  56257 MW;  CA2E580AC806B332 CRC64;
     MSLRYPISRI RHFRCQLREL LAAVAGPSIR IFDAGTGSLL SSWPPASYMR PLDSEISPDR
     ASSAGTCAEP PEKRRKLTPP VDESGEAQTE QSAKAKARKS QTAEQAWSTI PILVISGTGD
     HIVAVTGEDK CLRVFEVKQD GKLTQLTERC IPKRPCAISL TPDNNTILCG DKFGDVYSLP
     LLPRDEVVLP LRKAAESSKP FQPSATKLTV HTQRNLKALE QQLRTPRAAQ EKLEPSFQHR
     LLLGHVSMLT DLILAPVPSD SSTSPRLYII TSDRDEHIRV SRGPSQAHII HGYCLGHTSF
     VSKLCIPPWD PRSLISGGGD NCIICWDWLA GRVAQTIPLV HDGDVAESTK AQPPQSDAPD
     IAVSGIYAIP FSGNPGLTEH ASGGILVALE GVSRLLAFSF GTNGRLTALA PIELSGNALD
     VVALDDRGTI LVSVDNVHKP GSTKELRDSA IGPKLLQCFS ARSDGGLKWE ESSTQAATTI
     NSREAVDIIA ETESRKQVQK ISDSLYGIGN LRKWARGEDG