TRM82_CRYNJ
ID TRM82_CRYNJ Reviewed; 569 AA.
AC P0CS52; Q55PH3; Q5KDY5;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN Name=TRM82 {ECO:0000255|HAMAP-Rule:MF_03056}; OrderedLocusNames=CNG02370;
OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS MYA-565) (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=214684;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEC21 / ATCC MYA-565;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC 46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC induce conformational changes of the catalytic subunit.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBUNIT: Forms a heterodimer with the catalytic subunit TRM8.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC Rule:MF_03056}.
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DR EMBL; AE017347; AAW44655.1; -; Genomic_DNA.
DR RefSeq; XP_571962.1; XM_571962.1.
DR AlphaFoldDB; P0CS52; -.
DR SMR; P0CS52; -.
DR STRING; 5207.AAW44655; -.
DR PaxDb; P0CS52; -.
DR EnsemblFungi; AAW44655; AAW44655; CNG02370.
DR GeneID; 3258810; -.
DR KEGG; cne:CNG02370; -.
DR VEuPathDB; FungiDB:CNG02370; -.
DR eggNOG; KOG3914; Eukaryota.
DR HOGENOM; CLU_023695_0_0_1; -.
DR InParanoid; P0CS52; -.
DR OMA; THATVNT; -.
DR OrthoDB; 937275at2759; -.
DR UniPathway; UPA00989; -.
DR Proteomes; UP000002149; Chromosome 7.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043527; C:tRNA methyltransferase complex; IBA:GO_Central.
DR GO; GO:0106004; P:tRNA (guanine-N7)-methylation; IEA:GOC.
DR Gene3D; 2.130.10.10; -; 2.
DR HAMAP; MF_03056; TRM82; 1.
DR InterPro; IPR028884; Trm82.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR16288; PTHR16288; 1.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT CHAIN 1..569
FT /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT subunit TRM82"
FT /id="PRO_0000370519"
FT REPEAT 42..81
FT /note="WD 1"
FT REPEAT 147..188
FT /note="WD 2"
FT REPEAT 190..230
FT /note="WD 3"
FT REGION 522..569
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 569 AA; 61680 MW; C4A371A324D5FCB6 CRC64;
MALHCPPTAL ATSSTSLFIA AGPALHVFNP STSTFASCAA DGTGGLIRLL AVREDGEVAA
TLGEDKQLAV WDIQHDRPQF RHTRTVVKKG SCLSFAKDGS IILTDKVGDV YSYPLDPVPI
DPSAERPPMY TLVADPSQNP DATYLLGHVS MLNAHVMTPD GRRIITADRD EHIRVSRYPK
AYVIDKYLFG HDGFVSALHI PPSNPSLLLS AGGDPSLLIW NPSTGKLLTS VPVWPAVLSH
RRVRSHLRRH KPGSRKLKMD PVPEDMDEEN QTFYTAPEGY MLPSGQGVCI KKIESVKIGG
ETVVLFFSEG ASAIHSFILP TDPEGVKPTV HTLPLPHPLI DFTPIPSTSS ADEKEVLLSL
DTAWDVLKKN PGPGIEGRQD TIQRDDLSDE EKRNISKIFS VARISADGHL SLGDASVYDS
LVSNLPSTDI KTLSNVNLYP LLCVLPRWPG FEEGDEELTV NTGTPDTPGT PGPADAVSLA
PTSGAFLQKS YTQEELEKMN TKQLGRLKSA GIDVGSILLA RQKKAKEEHK ARKAAAKAKA
EAQKNQKEGG RPAKKKKKVE MSEEELANA