ID   TRM82_KLULA             Reviewed;         443 AA.
AC   Q6CQH2;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=TRM82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   OrderedLocusNames=KLLA0D17116g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit TRM8.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CR382124; CAH00913.1; -; Genomic_DNA.
DR   RefSeq; XP_453817.1; XM_453817.1.
DR   AlphaFoldDB; Q6CQH2; -.
DR   SMR; Q6CQH2; -.
DR   STRING; 28985.XP_453817.1; -.
DR   EnsemblFungi; CAH00913; CAH00913; KLLA0_D17116g.
DR   GeneID; 2893560; -.
DR   KEGG; kla:KLLA0_D17116g; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_022082_0_0_1; -.
DR   InParanoid; Q6CQH2; -.
DR   OMA; PPIYNYI; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0106143; C:tRNA (m7G46) methyltransferase complex; IEA:EnsemblFungi.
DR   GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:EnsemblFungi.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..443
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit TRM82"
FT                   /id="PRO_0000370521"
FT   REPEAT          97..137
FT                   /note="WD 1"
FT   REPEAT          193..235
FT                   /note="WD 2"
FT   REPEAT          239..279
FT                   /note="WD 3"
FT   REGION          67..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   443 AA;  50058 MW;  87BF09F73BCDE758 CRC64;
     MLHPFQSVLL NRDGSLLFCV VKNEIKAFKV EGNGYVLRGE WVDDLDNTPL IKEKVLKEQA
     RQLIENASKK LKTNDGEPVA QPKKQAKVPK PGPGAPPVYQ YIRNLALSRD GKLLLACTDS
     DKAAVIFNID LDDKDNIFKL IKRQPYPKRP NAITTSVDDK DLILADKFGD VYSMPIQNDV
     ITSINAEKAP ILGHVSMLTD VNMLTDSEGK QYIVTADRDE HIRISHYPQS FIVDKWLFGH
     EEFVSTICIP EWSDKLLFSA GGDKFVFSWN WKTGALLFKF DYTDLIQKYL TSDHLAPERF
     QNEKGDVIEY SVAKIVTLKD VPYIAFFVEA TKVLFVLKVD EKSGALSLHQ TLEFDEKIVS
     LTSALDVNTL CISLDNRDNQ DCDLVKLLLL EGDVFIEQKD TNSQLMNTIR STLKSDLIAN
     VEAGDVYPLY HNASLRKHGE HFS