ID   TRM82_LODEL             Reviewed;         389 AA.
AC   A5E654;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=TRM82 {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=LELG_05093;
OS   Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC
OS   1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade;
OC   Lodderomyces.
OX   NCBI_TaxID=379508;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11503 / BCRC 21390 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL
RC   YB-4239;
RX   PubMed=19465905; DOI=10.1038/nature08064;
RA   Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA   Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA   Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA   Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA   Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA   Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA   Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA   Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA   Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA   Birren B.W., Kellis M., Cuomo C.A.;
RT   "Evolution of pathogenicity and sexual reproduction in eight Candida
RT   genomes.";
RL   Nature 459:657-662(2009).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit TRM8.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CH981531; EDK46912.1; -; Genomic_DNA.
DR   RefSeq; XP_001523677.1; XM_001523627.1.
DR   AlphaFoldDB; A5E654; -.
DR   SMR; A5E654; -.
DR   STRING; 379508.A5E654; -.
DR   EnsemblFungi; EDK46912; EDK46912; LELG_05093.
DR   GeneID; 5230847; -.
DR   KEGG; lel:LELG_05093; -.
DR   VEuPathDB; FungiDB:LELG_05093; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_022082_0_0_1; -.
DR   InParanoid; A5E654; -.
DR   OMA; PPIYNYI; -.
DR   OrthoDB; 937275at2759; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000001996; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..389
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit TRM82"
FT                   /id="PRO_0000370522"
FT   REPEAT          44..86
FT                   /note="WD 1"
FT   REPEAT          134..179
FT                   /note="WD 2"
FT   REPEAT          184..222
FT                   /note="WD 3"
SQ   SEQUENCE   389 AA;  43900 MW;  F4F4818CDE2A7B0E CRC64;
     MKHPFQILTS STDGKLLIAS ASSPSKESSL LLLLPELGDV LCQQNVPQPI YINYLETGPD
     KVLITADVDK NITIYKLENN ELHQLKQQQM PKRLSGISTL NNDAIVCDSL GDVYQITIDT
     QPAVKKEDLK PLLGHTSPLT AVVAAQYKNP PKSFLITSDR DEHIRVSNYP KSYVIKGFLY
     GHTQFVSQIH LFEICKESRL VSGGGEGKLF FWDWFREVLI TEFDLMPYVE EYLHEFHIKK
     SSKTKEEQDV QKEQEVENIQ PKYEIGVQKI DSIEGEDGVF IVTLVENTSC LVVVHFTDEA
     KHAQTIQLAA PAVTFAIVGN KLFVSLDAEN DNILTIYKFV DGQFVNDDEA QSSIAERIIL
     ANPINVEDKS KFAPFYSINQ LRKRGNNYS