TRM82_PICGU
ID TRM82_PICGU Reviewed; 420 AA.
AC A5DNM7;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN Name=TRM82 {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=PGUG_04878;
OS Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539
OS / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Meyerozyma.
OX NCBI_TaxID=294746;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324;
RX PubMed=19465905; DOI=10.1038/nature08064;
RA Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA Birren B.W., Kellis M., Cuomo C.A.;
RT "Evolution of pathogenicity and sexual reproduction in eight Candida
RT genomes.";
RL Nature 459:657-662(2009).
CC -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC 46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC induce conformational changes of the catalytic subunit.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBUNIT: Forms a heterodimer with the catalytic subunit TRM8.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC Rule:MF_03056}.
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DR EMBL; CH408160; EDK40780.1; -; Genomic_DNA.
DR RefSeq; XP_001482923.1; XM_001482873.1.
DR AlphaFoldDB; A5DNM7; -.
DR SMR; A5DNM7; -.
DR STRING; 4929.XP_001482923.1; -.
DR EnsemblFungi; EDK40780; EDK40780; PGUG_04878.
DR GeneID; 5124856; -.
DR KEGG; pgu:PGUG_04878; -.
DR VEuPathDB; FungiDB:PGUG_04878; -.
DR eggNOG; KOG3914; Eukaryota.
DR HOGENOM; CLU_022082_0_0_1; -.
DR InParanoid; A5DNM7; -.
DR OMA; PPIYNYI; -.
DR OrthoDB; 937275at2759; -.
DR UniPathway; UPA00989; -.
DR Proteomes; UP000001997; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03056; TRM82; 1.
DR InterPro; IPR028884; Trm82.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR16288; PTHR16288; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT CHAIN 1..420
FT /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT subunit TRM82"
FT /id="PRO_0000370524"
FT REPEAT 87..127
FT /note="WD 1"
FT REPEAT 179..220
FT /note="WD 2"
FT REPEAT 224..266
FT /note="WD 3"
FT REPEAT 340..379
FT /note="WD 4"
FT REGION 59..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 420 AA; 47995 MW; 03E25D9744A9E0CF CRC64;
MKHPFQIIVA DTTGRYLFTT VKNVLLVIDL TSGKLVGQWK DELDNSDFLK KKYEEKFDKE
NDSKRQKSES GQAKVSKIPT PGPGAPPIYN YIRALRLSSD EKYLFATTDS DKAVVIFTID
HSKDNCLELK KRQPFSKRPC ALSVDEDRLV VADKFGDVYS IAIEDDQVVN EKELTPILGH
VSMLSDVAIV SNDKKKFIIT GDRDEHIRIS NYPKSFVIKN FLFGHHEFVS CLHVPNFDQS
LLISGGGDDY LCLWRWYEGQ LVAKIELREL IAPFLTDSHL PPERFLTETS PKEISVARIL
TFTNKKTGLN ILVVLCEQTK AVLLFEIDAK LSVRHLQTIS YEDNVLDMCY IEATNTLISA
HDSDKNQLFQ QQKFDEETNN FTPTTETMDA VAELNPIDVN SRNDFLPLYY INTLRKRSEH
