ID   TRM82_PICST             Reviewed;         445 AA.
AC   A3LPG0;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=TRM82 {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=PICST_56344;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit TRM8.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CP000496; ABN65038.2; -; Genomic_DNA.
DR   RefSeq; XP_001383067.2; XM_001383030.1.
DR   AlphaFoldDB; A3LPG0; -.
DR   SMR; A3LPG0; -.
DR   STRING; 4924.XP_001383067.2; -.
DR   EnsemblFungi; ABN65038; ABN65038; PICST_56344.
DR   GeneID; 4837062; -.
DR   KEGG; pic:PICST_56344; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_022082_0_0_1; -.
DR   InParanoid; A3LPG0; -.
DR   OMA; PPIYNYI; -.
DR   OrthoDB; 937275at2759; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000002258; Chromosome 2.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   SMART; SM00320; WD40; 2.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..445
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit TRM82"
FT                   /id="PRO_0000370525"
FT   REPEAT          109..149
FT                   /note="WD 1"
FT   REPEAT          205..243
FT                   /note="WD 2"
FT   REPEAT          247..289
FT                   /note="WD 3"
FT   REGION          60..103
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        60..93
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   445 AA;  50672 MW;  D0DC3E441113546F CRC64;
     MKHPFQIVLV NKAGTHLFAS VKNHLQVFRL SDGKVVGSWI DTVDMFTPLK KQQEEKIKVL
     QEQNKQTEES ENNEIKNESA EPAEKKSKGN DSKPIKIPKI PVPGPGAPPI YNYIRSLALS
     EDEKHLMGTT DSDKAAIIFK VDLSSEGNCL TLIKRQVFQK RPCAVSSDQE TLIVADKFGD
     VYSIGIDNEA PVDEKELSPI LGHVSMLSDV LVATYNDKKF ILTGDRDEHI RVSNYPKSYV
     IKNWLFGHHE FVSNLHIPEF DSSLLVSGGG DDYLCLWKWY DNQLLSKIEL RDLLLPFLEE
     FHLPPERFLT DESQKEISIA KILTFVSKKS QKKYIVVLAE NTKCLLTFEL KADNTVTHKQ
     TLTTDFPIID ICLISENSSI IAAVDRETSD SLLEFYTLGD EDVFEVQSHT DIIQTITSAN
     ECDVEKRREF YPLYYINTLR KRSEH