ID   TRM82_SCLS1             Reviewed;         517 AA.
AC   A7F9K1;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit trm82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=trm82; ORFNames=SS1G_14282;
OS   Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold)
OS   (Whetzelinia sclerotiorum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC   Helotiales; Sclerotiniaceae; Sclerotinia.
OX   NCBI_TaxID=665079;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 18683 / 1980 / Ss-1;
RX   PubMed=21876677; DOI=10.1371/journal.pgen.1002230;
RA   Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A.,
RA   Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E.,
RA   Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M.,
RA   Quevillon E., Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P.,
RA   Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I.,
RA   Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P.,
RA   Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C.,
RA   Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C.,
RA   Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E.,
RA   Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N.,
RA   Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E.,
RA   Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O.,
RA   Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.;
RT   "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia
RT   sclerotiorum and Botrytis cinerea.";
RL   PLoS Genet. 7:E1002230-E1002230(2011).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit trm8.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CH476651; EDO00412.1; -; Genomic_DNA.
DR   RefSeq; XP_001584827.1; XM_001584777.1.
DR   AlphaFoldDB; A7F9K1; -.
DR   SMR; A7F9K1; -.
DR   STRING; 665079.A7F9K1; -.
DR   EnsemblFungi; EDO00412; EDO00412; SS1G_14282.
DR   GeneID; 5480859; -.
DR   KEGG; ssl:SS1G_14282; -.
DR   VEuPathDB; FungiDB:sscle_04g039750; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_022082_0_0_1; -.
DR   InParanoid; A7F9K1; -.
DR   OMA; WVFEHDG; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000001312; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0043527; C:tRNA methyltransferase complex; IBA:GO_Central.
DR   GO; GO:0106004; P:tRNA (guanine-N7)-methylation; IEA:GOC.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..517
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit trm82"
FT                   /id="PRO_0000370526"
FT   REPEAT          116..158
FT                   /note="WD 1"
FT   REPEAT          260..301
FT                   /note="WD 2"
FT   REPEAT          306..346
FT                   /note="WD 3"
FT   REGION          40..117
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        93..117
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   517 AA;  56911 MW;  1888F0E25539DC58 CRC64;
     MSILRSPYQC LKQCGNYLVA ARGSSIDTFD IKNGSYLSTW KSPVPESMSR SKTTEEETQT
     KNEDQNSETA TPEFILESSA PPAKRRKLSI TKESGENTGV VQQSKKKTKN SSPKILEPSP
     ITALTITRDL QHVIAVTGED KTIRVLAWED TVEKGLRQIS DRTMPKRPCA LAITDDCNTI
     ISADKFGDVY SLPLIPSPLV PSATENASVQ KQAPKMFQPS ASALTVHSAR NLKALEAQKK
     QSNKVSEKTG PDFEHKLLLG HVSMLTDILV ATLSGRQYIL TADRDEHIRI SRGIPQAHII
     ENFCLGHIEY VSRLCIPPTR PEILISGGGD DDLYTWNWLN GSLLSKTNLK SQVEALDTEK
     QSAQEAESKK IAVTGVYHAR DEVSNQDIII ATSEGVPAAF IYFLTASNQL THTQTLALPG
     NALSCTFSNP DLSSPFSLII SINNIHEPSS ITTLKDANSS VANPLQFFKY ENEKFVSVQQ
     DGFAPQDSEG ILDDQQKNNL CGLLYNVGNL RKMEDEE