ID   TRM82_VANPO             Reviewed;         453 AA.
AC   A7TEN6;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=TRM82 {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=Kpol_1036p90;
OS   Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS
OS   2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma.
OX   NCBI_TaxID=436907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL
RC   Y-8283 / UCD 57-17;
RX   PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA   Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.;
RT   "Independent sorting-out of thousands of duplicated gene pairs in two yeast
RT   species descended from a whole-genome duplication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit TRM8.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; DS480380; EDO19343.1; -; Genomic_DNA.
DR   RefSeq; XP_001647201.1; XM_001647151.1.
DR   AlphaFoldDB; A7TEN6; -.
DR   SMR; A7TEN6; -.
DR   STRING; 436907.A7TEN6; -.
DR   EnsemblFungi; EDO19343; EDO19343; Kpol_1036p90.
DR   GeneID; 5547683; -.
DR   KEGG; vpo:Kpol_1036p90; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_022082_0_0_1; -.
DR   InParanoid; A7TEN6; -.
DR   OMA; PPIYNYI; -.
DR   OrthoDB; 937275at2759; -.
DR   PhylomeDB; A7TEN6; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000000267; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   SMART; SM00320; WD40; 2.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..453
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit TRM82"
FT                   /id="PRO_0000370527"
FT   REPEAT          103..143
FT                   /note="WD 1"
FT   REPEAT          244..286
FT                   /note="WD 2"
FT   REGION          69..99
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        69..94
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   453 AA;  51733 MW;  31800D0495DFD9A3 CRC64;
     MSLIHPIQAV VSNRSGELIF AVLKNVIIAY RYDQTNGKYL EMGKWEDQFS RDEMIKIAVV
     KEQARQLAEN EEKGIKKSKT NEGNTIEKKH DAKIPVPGPG APPIYSQIRN LMISNDETML
     LACADSDKSV LVFKIETLNN DNCLKLIKRQ PFPKRPNAIT ITEDDKTVII ADKFGDVYSM
     LIESPVIENI DEEFEPILGH VSMLTGVLST TNNGMKFVMT SDRDEHIKIS HFPQSYIVDK
     WLFGHKEFVS SICIPSWNSN ILVSAGGDHG IFLWDWIKGE KLDEFDFTDL VLPYINENHL
     APDRFQNEEN DLKEFAVSKL VSLPNNPYFA FFVEATKLLI ILEVDQSTYK MKLLQKIVLP
     YYITFISTFS DDKTMGFNIS LDNRESGDKD FVKFIALDTT SNTFSIKEEQ SDEFNTSIVN
     SFSKEDSIVK VELDEVYPLY NIISLKKHGE HYS