TRM82_YEAS7
ID TRM82_YEAS7 Reviewed; 444 AA.
AC A6ZYC3;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 {ECO:0000255|HAMAP-Rule:MF_03056};
DE AltName: Full=Transfer RNA methyltransferase 82 {ECO:0000255|HAMAP-Rule:MF_03056};
GN Name=TRM82 {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=SCY_1061;
OS Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=307796;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YJM789;
RX PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA Steinmetz L.M.;
RT "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT strain YJM789.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC 46 (m7G46) in tRNA, a modification required to maintain stability of
CC tRNAs; its absence resulting in tRNA decay. In the complex, it is
CC required to stabilize and induce conformational changes of the
CC catalytic subunit. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBUNIT: Forms a heterodimer with the catalytic subunit TRM8.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC Rule:MF_03056}.
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DR EMBL; AAFW02000145; EDN60503.1; -; Genomic_DNA.
DR AlphaFoldDB; A6ZYC3; -.
DR SMR; A6ZYC3; -.
DR PRIDE; A6ZYC3; -.
DR EnsemblFungi; EDN60503; EDN60503; SCY_1061.
DR HOGENOM; CLU_022082_0_0_1; -.
DR UniPathway; UPA00989; -.
DR Proteomes; UP000007060; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03056; TRM82; 1.
DR InterPro; IPR028884; Trm82.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR16288; PTHR16288; 1.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Nucleus; Phosphoprotein; Repeat; tRNA processing; WD repeat.
FT CHAIN 1..444
FT /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT subunit TRM82"
FT /id="PRO_0000370531"
FT REPEAT 1..47
FT /note="WD 1"
FT REPEAT 48..99
FT /note="WD 2"
FT REPEAT 100..147
FT /note="WD 3"
FT REPEAT 148..192
FT /note="WD 4"
FT REPEAT 193..237
FT /note="WD 5"
FT REPEAT 238..279
FT /note="WD 6"
FT REPEAT 308..354
FT /note="WD 7"
FT REGION 55..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..84
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 93
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q03774"
SQ SEQUENCE 444 AA; 50429 MW; 5E8F73874E50DC76 CRC64;
MSVIHPLQNL LTSRDGSLVF AIIKNCILSF KYQSPNHWEF AGKWSDDFDK IQESRNTTAK
EQQGQSSENE NENKKLKSNK GDSIKRTAAK VPSPGIGAPP IYSYIRNLRL TSDESRLIAC
ADSDKSLLVF DVDKTSKNVL KLRKRFCFSK RPNAISIAED DTTVIIADKF GDVYSIDINS
IPEEKFTQEP ILGHVSMLTD VHLIKDSDGH QFIITSDRDE HIKISHYPQC FIVDKWLFGH
KHFVSSICCG KDYLLLSAGG DDKIFAWDWK TGKNLSTFDY SSLIKPYLND QHLAPPRFQN
ENNDIIEFAV SKIIKSKNLP FVAFFVEATK CIIILEISEK QKGDLALKQI ISFPYNVISL
SAHNDEFQVT LDNKESSGVQ KNFAKFIEYN LNENSFVVNN EKSNEFDSAI IQSVQGDSNL
VTKKEEIYPL YNVSSLRKHG EHYS