ID   BURS_APIME              Reviewed;         153 AA.
AC   A2VB89; Q32TG6; Q566B0;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Bursicon;
DE   AltName: Full=Bursicon subunit alpha;
DE   AltName: Full=Single-chain bursicon;
DE   Flags: Precursor;
GN   Name=burs {ECO:0000312|EMBL:CAM06631.1};
GN   Synonyms=burs-alpha {ECO:0000303|PubMed:17275819},
GN   sc-burs {ECO:0000303|PubMed:15811337};
OS   Apis mellifera (Honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=7460;
RN   [1] {ECO:0000312|EMBL:CAM06631.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Larva {ECO:0000269|PubMed:17275819};
RX   PubMed=17275819; DOI=10.1016/j.ygcen.2006.12.004;
RA   Van Loy T., Van Hiel M.B., Vandersmissen H.P., Poels J., Mendive F.M.,
RA   Vassart G., Vanden Broeck J.J.M.;
RT   "Evolutionary conservation of bursicon in the animal kingdom.";
RL   Gen. Comp. Endocrinol. 153:59-63(2007).
RN   [2] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DH4;
RG   Honey bee genome project;
RA   Zhang L., Deng J., Wu Y.-Q., Kovar C., Aqrawi P., Bandaranaike D.,
RA   Blankenburg K., Chen D., Denson S., Dinh H., Firestine M., Gross S.,
RA   Han Y., Hernandez B., Holder M., Jackson L., Javaid M., Jing C., Jones J.,
RA   Joshi V., Kamau G., Korchina V., Lee S., Lorensuhewa L., Mata R.,
RA   Mathew T., Mims S., Ngo R., Nguyen L., Okwuonu G., Ongeri F., Osuji N.,
RA   Pham C., Puazo M., Qu C., Quiroz J., Raj R., Rio Deiros D., Santibanez J.,
RA   Scheel M., Scherer S., Vee V., Wang M., Xin Y., Richards S., Reid J.G.,
RA   Newsham I., Worley K.C., Muzny D.M., Gibbs R.;
RL   Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000305, ECO:0000312|EMBL:CAH89263.2}
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=15811337; DOI=10.1016/j.febslet.2005.03.006;
RA   Mendive F.M., Van Loy T., Claeysen S., Poels J., Williamson M., Hauser F.,
RA   Grimmelikhuijzen C.J.P., Vassart G., Vanden Broeck J.J.M.;
RT   "Drosophila molting neurohormone bursicon is a heterodimer and the natural
RT   agonist of the orphan receptor DLGR2.";
RL   FEBS Lett. 579:2171-2176(2005).
CC   -!- FUNCTION: Final heterodimeric neurohormone released at the end of the
CC       molting cycle, involved in the sclerotization (tanning) of the insect
CC       cuticle, melanization and wing spreading.
CC       {ECO:0000269|PubMed:15811337}.
CC   -!- SUBUNIT: Heterodimer of burs and pburs. {ECO:0000250|UniProtKB:Q9VD83}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VD83}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAH89263.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AM420631; CAM06631.1; -; mRNA.
DR   EMBL; AADG06006208; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BN000692; CAH89263.2; ALT_SEQ; mRNA.
DR   RefSeq; NP_001091704.1; NM_001098234.1.
DR   AlphaFoldDB; A2VB89; -.
DR   STRING; 7460.GB45446-PA; -.
DR   PaxDb; A2VB89; -.
DR   EnsemblMetazoa; NM_001098234; NP_001091704; GeneID_100049551.
DR   GeneID; 100049551; -.
DR   KEGG; ame:100049551; -.
DR   CTD; 42560; -.
DR   eggNOG; KOG1216; Eukaryota.
DR   HOGENOM; CLU_132265_0_0_1; -.
DR   InParanoid; A2VB89; -.
DR   OMA; FACTGRC; -.
DR   PhylomeDB; A2VB89; -.
DR   Proteomes; UP000005203; Unplaced.
DR   GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; IMP:UniProtKB.
DR   GO; GO:0007593; P:chitin-based cuticle sclerotization; IMP:UniProtKB.
DR   GO; GO:0048067; P:cuticle pigmentation; IMP:UniProtKB.
DR   Gene3D; 2.10.90.10; -; 1.
DR   InterPro; IPR006207; Cys_knot_C.
DR   InterPro; IPR029034; Cystine-knot_cytokine.
DR   InterPro; IPR004133; DAN.
DR   Pfam; PF03045; DAN; 1.
DR   PROSITE; PS01225; CTCK_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..153
FT                   /note="Bursicon"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5000223743"
FT   DOMAIN          29..119
FT                   /note="CTCK"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        29..78
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        43..92
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        53..113
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        57..115
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        75..118
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        77
FT                   /note="Interchain"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
SQ   SEQUENCE   153 AA;  16943 MW;  A268E866C000C153 CRC64;
     MLLYHIVGAS VLICLLNETA KAIIGVDECQ ATPVIHFLQY PGCVPKPIPS YACRGRCSSY
     LQVSGSKIWQ MERSCMCCQE SGEREASVSL FCPRAKPGEK KFRKVITKAP LECMCRPCTS
     VEEYAIIPQE IAGFADEGPF TTSAHFRRSS DLQ