ID   BURS_PERAM              Reviewed;          81 AA.
AC   P84118;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   29-SEP-2021, entry version 60.
DE   RecName: Full=Bursicon;
DE   AltName: Full=Bursicon subunit alpha;
DE   AltName: Full=Cuticle-tanning hormone;
DE   Flags: Fragments;
GN   Name=burs;
OS   Periplaneta americana (American cockroach) (Blatta americana).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Polyneoptera; Dictyoptera; Blattodea; Blattoidea; Blattidae;
OC   Blattinae; Periplaneta.
OX   NCBI_TaxID=6978;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 1-41 AND 66-81.
RC   TISSUE=Ventral nerve cord {ECO:0000269|PubMed:10528407};
RX   PubMed=10528407; DOI=10.1016/s0965-1748(99)00060-0;
RA   Kostron B., Market D., Kellermann J., Carter C.E., Honegger H.W.;
RT   "Antisera against Periplaneta americana Cu,Zn-superoxide dismutase (SOD):
RT   separation of the neurohormone bursicon from SOD, and immunodetection of
RT   SOD in the central nervous system.";
RL   Insect Biochem. Mol. Biol. 29:861-871(1999).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 42-65, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND
RP   TISSUE SPECIFICITY.
RC   TISSUE=Ventral nerve cord {ECO:0000269|PubMed:12271490};
RX   PubMed=12271490; DOI=10.1002/cne.10357;
RA   Honegger H.W., Market D., Pierce L.A., Dewey E.M., Kostron B., Wilson M.,
RA   Choi D., Klukas K.A., Mesce K.A.;
RT   "Cellular localization of bursicon using antisera against partial peptide
RT   sequences of this insect cuticle-sclerotizing neurohormone.";
RL   J. Comp. Neurol. 452:163-177(2002).
RN   [3]
RP   TISSUE SPECIFICITY.
RX   PubMed=15703293; DOI=10.1073/pnas.0409916102;
RA   Luo C.-W., Dewey E.M., Sudo S., Ewer J., Hsu S.Y., Honegger H.-W.,
RA   Hsueh A.J.W.;
RT   "Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine
RT   knot protein that activates G protein-coupled receptor LGR2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:2820-2825(2005).
CC   -!- FUNCTION: Final heterodimeric neurohormone released at the end of the
CC       molting cycle, involved in the sclerotization (tanning) of the insect
CC       cuticle, melanization and wing spreading.
CC       {ECO:0000269|PubMed:12271490}.
CC   -!- SUBUNIT: Heterodimer of burs and pburs. {ECO:0000269|PubMed:12271490}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12271490}.
CC   -!- TISSUE SPECIFICITY: Central nervous system. Coexpressed with CCAP in
CC       most CCAP-specific neurons. Coexpressed with pburs in the large
CC       bilateral lateral neurosecretory neurons of the first three unfused
CC       abdominal ganglia and in all anterior bilateral cell pairs in the
CC       thoracic ganglia. {ECO:0000269|PubMed:12271490,
CC       ECO:0000269|PubMed:15703293}.
CC   -!- CAUTION: The order of the 5th peptide within the sequence is unknown.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; NAS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0007593; P:chitin-based cuticle sclerotization; NAS:UniProtKB.
DR   GO; GO:0048067; P:cuticle pigmentation; NAS:UniProtKB.
DR   GO; GO:0006801; P:superoxide metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.40.200; -; 1.
DR   InterPro; IPR036423; SOD-like_Cu/Zn_dom_sf.
DR   InterPro; IPR018152; SOD_Cu/Zn_BS.
DR   InterPro; IPR001424; SOD_Cu_Zn_dom.
DR   Pfam; PF00080; Sod_Cu; 1.
DR   SUPFAM; SSF49329; SSF49329; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hormone; Secreted.
FT   CHAIN           <1..>81
FT                   /note="Bursicon"
FT                   /id="PRO_0000065024"
FT   NON_CONS        25..26
FT                   /evidence="ECO:0000303|PubMed:10528407"
FT   NON_CONS        38..39
FT                   /evidence="ECO:0000303|PubMed:10528407"
FT   NON_CONS        41..42
FT                   /evidence="ECO:0000303|PubMed:10528407,
FT                   ECO:0000303|PubMed:12271490"
FT   NON_CONS        53..54
FT                   /evidence="ECO:0000303|PubMed:10528407,
FT                   ECO:0000303|PubMed:12271490"
FT   NON_CONS        65..66
FT                   /evidence="ECO:0000303|PubMed:10528407,
FT                   ECO:0000303|PubMed:12271490"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|PubMed:10528407"
FT   NON_TER         81
FT                   /evidence="ECO:0000303|PubMed:10528407"
SQ   SEQUENCE   81 AA;  8392 MW;  1E60D18899354265 CRC64;
     GTVFFDQDSP DSPVKVTGEV TGLQKHGFHI HEFGDNTNMS ADGSSYLQVS GSKLTQEGQA
     SMEVKGNAGA RVAXGVVGIA K