BUT82_GIBZE
ID BUT82_GIBZE Reviewed; 177 AA.
AC I1RV22;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Putative acetyltransferase FG08082 {ECO:0000303|PubMed:17175185};
DE EC=2.3.1.- {ECO:0000255|PROSITE-ProRule:PRU00532};
DE AltName: Full=Butenolide biosynthesis cluster protein FG08082 {ECO:0000303|PubMed:17175185};
GN ORFNames=FG08082, FGRAMPH1_01T09073;
OS Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=229533;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=17823352; DOI=10.1126/science.1143708;
RA Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT "The Fusarium graminearum genome reveals a link between localized
RT polymorphism and pathogen specialization.";
RL Science 317:1400-1402(2007).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA Hammond-Kosack K.E.;
RT "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT graminearum.";
RL BMC Genomics 16:544-544(2015).
RN [4]
RP FUNCTION, INDUCTION, AND PATHWAY.
RX PubMed=17175185; DOI=10.1016/j.fgb.2006.11.001;
RA Harris L.J., Alexander N.J., Saparno A., Blackwell B., McCormick S.P.,
RA Desjardins A.E., Robert L.S., Tinker N., Hattori J., Piche C.,
RA Schernthaner J.P., Watson R., Ouellet T.;
RT "A novel gene cluster in Fusarium graminearum contains a gene that
RT contributes to butenolide synthesis.";
RL Fungal Genet. Biol. 44:293-306(2007).
CC -!- FUNCTION: Putative acetyltransferase; part of the gene cluster that
CC mediates the biosynthesis of butenolide, a mycotoxin that shows
CC antibiotic activity but does not seem to play a major role in the
CC spread of head blight in wheat (PubMed:17175185). Butenolide is derived
CC from glutamic acid via a 4-acetamido-2-butenoic acid intermediate
CC (Probable). The predicted function of the NADH:flavin oxidoreductase
CC FG08077, the cytochrome P450 monooxygenase FG08079, the decarboxylase
CC FG08083, and the putative acetyltransferase FG08082 are consistent with
CC this pathway, however, the respective activities of the butelonide
CC biosynthesis cluster enzymes have still to be experimentally determined
CC (Probable). {ECO:0000269|PubMed:17175185, ECO:0000305|PubMed:17175185}.
CC -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000305|PubMed:17175185}.
CC -!- INDUCTION: Highly expressed under trichothecene-producing conditions.
CC {ECO:0000269|PubMed:17175185}.
CC -!- SIMILARITY: Belongs to the acetyltransferase family. {ECO:0000305}.
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DR EMBL; HG970333; CEF76339.1; -; Genomic_DNA.
DR RefSeq; XP_011320740.1; XM_011322438.1.
DR AlphaFoldDB; I1RV22; -.
DR SMR; I1RV22; -.
DR GeneID; 23555116; -.
DR KEGG; fgr:FGSG_08082; -.
DR VEuPathDB; FungiDB:FGRAMPH1_01G09073; -.
DR eggNOG; ENOG502SYW6; Eukaryota.
DR HOGENOM; CLU_086503_2_0_1; -.
DR InParanoid; I1RV22; -.
DR Proteomes; UP000070720; Chromosome 2.
DR GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR000182; GNAT_dom.
DR Pfam; PF00583; Acetyltransf_1; 1.
DR SUPFAM; SSF55729; SSF55729; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Reference proteome; Transferase.
FT CHAIN 1..177
FT /note="Putative acetyltransferase FG08082"
FT /id="PRO_0000450727"
FT DOMAIN 81..174
FT /note="N-acetyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
SQ SEQUENCE 177 AA; 19949 MW; 5894868E6A1B5706 CRC64;
MINITKPQNW TRGDYLVSTD PALLQVDAIN AALSSDMVWW AGDLPADALW DALRSSICFG
LYRKRSSEMN GTGPTVEYKI EEWEQVGLVR MITDGVTFGY LTDVYILPEH QGGGRGRWML
QILNEALQGW PHLRRVMLLT TDKMHLFGKN LGMKDYREFD GMKGVSIAMV EGPGAQH
