ID   BWNIN_DANRE             Reviewed;          65 AA.
AC   E7EXZ6;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   08-MAR-2011, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Protein brawnin {ECO:0000303|PubMed:32161263};
GN   Name=brawnin {ECO:0000303|PubMed:32161263};
GN   Synonyms=br {ECO:0000303|PubMed:32161263}, bsi:ch211-68a17.7;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=32161263; DOI=10.1038/s41467-020-14999-2;
RA   Zhang S., Reljic B., Liang C., Kerouanton B., Francisco J.C., Peh J.H.,
RA   Mary C., Jagannathan N.S., Olexiouk V., Tang C., Fidelito G., Nama S.,
RA   Cheng R.K., Wee C.L., Wang L.C., Duek Roggli P., Sampath P., Lane L.,
RA   Petretto E., Sobota R.M., Jesuthasan S., Tucker-Kellogg L., Reversade B.,
RA   Menschaert G., Sun L., Stroud D.A., Ho L.;
RT   "Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory
RT   complex III assembly.";
RL   Nat. Commun. 11:1312-1312(2020).
CC   -!- FUNCTION: Essential for mitochondrial respiratory chain complex III
CC       (CIII) assembly and stability (PubMed:32161263). Also required for
CC       cytochrome c oxidase complex (complex IV) assembly (By similarity).
CC       {ECO:0000250|UniProtKB:Q69YU5, ECO:0000269|PubMed:32161263}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000305|PubMed:32161263}; Single-pass type II membrane protein
CC       {ECO:0000250|UniProtKB:Q69YU5}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in skeletal and cardiac muscle (at
CC       protein level). {ECO:0000269|PubMed:32161263}.
CC   -!- DISRUPTION PHENOTYPE: A complete loss of mitochondrial respiratory
CC       chain complex III resulting in severe growth retardation, lactic
CC       acidosis and early death. {ECO:0000269|PubMed:32161263}.
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DR   EMBL; CR391963; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001129045.1; NM_001135573.2.
DR   AlphaFoldDB; E7EXZ6; -.
DR   STRING; 7955.ENSDARP00000129487; -.
DR   PaxDb; E7EXZ6; -.
DR   Ensembl; ENSDART00000155927; ENSDARP00000129487; ENSDARG00000045797.
DR   GeneID; 100190888; -.
DR   KEGG; dre:100190888; -.
DR   ZFIN; ZDB-GENE-100427-3; si:ch211-68a17.7.
DR   eggNOG; ENOG502S9EP; Eukaryota.
DR   GeneTree; ENSGT00390000007685; -.
DR   HOGENOM; CLU_202878_0_0_1; -.
DR   InParanoid; E7EXZ6; -.
DR   OMA; PRYLKMF; -.
DR   OrthoDB; 1635247at2759; -.
DR   PhylomeDB; E7EXZ6; -.
DR   PRO; PR:E7EXZ6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 25.
DR   Bgee; ENSDARG00000045797; Expressed in heart and 23 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0034551; P:mitochondrial respiratory chain complex III assembly; IMP:UniProtKB.
DR   InterPro; IPR027858; BRAWNIN.
DR   PANTHER; PTHR28492; PTHR28492; 1.
DR   Pfam; PF14990; DUF4516; 1.
PE   1: Evidence at protein level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Signal-anchor; Transmembrane; Transmembrane helix.
FT   CHAIN           1..65
FT                   /note="Protein brawnin"
FT                   /id="PRO_0000450548"
FT   TOPO_DOM        1..9
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000250|UniProtKB:Q69YU5"
FT   TRANSMEM        10..32
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        33..65
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000250|UniProtKB:Q69YU5"
SQ   SEQUENCE   65 AA;  7427 MW;  F7BD2ADD04153822 CRC64;
     MPAGVSWPRY LRMFAASVLS MFAGAQVVHH YYRPDLSIPE IPPKPGELRT ELLGLKERQM
     DSQKQ