BWNIN_MOUSE
ID BWNIN_MOUSE Reviewed; 67 AA.
AC Q8BTC1;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Protein BRAWNIN {ECO:0000303|PubMed:32161263};
GN Name=Brawnin {ECO:0000303|PubMed:32161263};
GN Synonyms=Br {ECO:0000303|PubMed:32161263};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INTERACTION WITH
RP UQCRC1 AND UQCRQ.
RX PubMed=32161263; DOI=10.1038/s41467-020-14999-2;
RA Zhang S., Reljic B., Liang C., Kerouanton B., Francisco J.C., Peh J.H.,
RA Mary C., Jagannathan N.S., Olexiouk V., Tang C., Fidelito G., Nama S.,
RA Cheng R.K., Wee C.L., Wang L.C., Duek Roggli P., Sampath P., Lane L.,
RA Petretto E., Sobota R.M., Jesuthasan S., Tucker-Kellogg L., Reversade B.,
RA Menschaert G., Sun L., Stroud D.A., Ho L.;
RT "Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory
RT complex III assembly.";
RL Nat. Commun. 11:1312-1312(2020).
CC -!- FUNCTION: Essential for mitochondrial respiratory chain complex III
CC (CIII) assembly and stability (PubMed:32161263). Also required for
CC cytochrome c oxidase complex (complex IV) assembly (By similarity).
CC {ECO:0000250|UniProtKB:Q69YU5, ECO:0000269|PubMed:32161263}.
CC -!- SUBUNIT: Interacts with UQCRC1 (PubMed:32161263). Interacts with UQCRQ
CC (PubMed:32161263). Interacts with SMIM4 (By similarity).
CC {ECO:0000250|UniProtKB:Q69YU5, ECO:0000269|PubMed:32161263}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:32161263}; Single-pass type II membrane protein
CC {ECO:0000250|UniProtKB:Q69YU5}.
CC -!- TISSUE SPECIFICITY: Highly expressed in brown adipose, cardiac and
CC skeletal muscle (at protein level). {ECO:0000269|PubMed:32161263}.
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DR EMBL; AK004515; BAC25085.1; -; mRNA.
DR CCDS; CCDS48653.1; -.
DR RefSeq; NP_001129039.1; NM_001135567.1.
DR RefSeq; NP_001129040.1; NM_001135568.1.
DR RefSeq; NP_001129041.1; NM_001135569.1.
DR AlphaFoldDB; Q8BTC1; -.
DR SMR; Q8BTC1; -.
DR STRING; 10090.ENSMUSP00000078593; -.
DR iPTMnet; Q8BTC1; -.
DR PhosphoSitePlus; Q8BTC1; -.
DR MaxQB; Q8BTC1; -.
DR PaxDb; Q8BTC1; -.
DR PeptideAtlas; Q8BTC1; -.
DR PRIDE; Q8BTC1; -.
DR Ensembl; ENSMUST00000079648; ENSMUSP00000078593; ENSMUSG00000063320.
DR Ensembl; ENSMUST00000176200; ENSMUSP00000134868; ENSMUSG00000063320.
DR Ensembl; ENSMUST00000183363; ENSMUSP00000139234; ENSMUSG00000063320.
DR Ensembl; ENSMUST00000185168; ENSMUSP00000139244; ENSMUSG00000063320.
DR GeneID; 544717; -.
DR KEGG; mmu:544717; -.
DR UCSC; uc007gjp.2; mouse.
DR MGI; MGI:3698433; 1190007I07Rik.
DR VEuPathDB; HostDB:ENSMUSG00000063320; -.
DR eggNOG; ENOG502S9EP; Eukaryota.
DR GeneTree; ENSGT00940000173873; -.
DR HOGENOM; CLU_202878_0_0_1; -.
DR InParanoid; Q8BTC1; -.
DR OMA; PRYLKMF; -.
DR OrthoDB; 1635247at2759; -.
DR PhylomeDB; Q8BTC1; -.
DR TreeFam; TF333420; -.
DR BioGRID-ORCS; 544717; 3 hits in 76 CRISPR screens.
DR PRO; PR:Q8BTC1; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q8BTC1; protein.
DR Bgee; ENSMUSG00000063320; Expressed in quadriceps femoris and 64 other tissues.
DR ExpressionAtlas; Q8BTC1; baseline and differential.
DR Genevisible; Q8BTC1; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0034551; P:mitochondrial respiratory chain complex III assembly; IMP:UniProtKB.
DR InterPro; IPR027858; BRAWNIN.
DR PANTHER; PTHR28492; PTHR28492; 1.
DR Pfam; PF14990; DUF4516; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..67
FT /note="Protein BRAWNIN"
FT /id="PRO_0000340274"
FT TOPO_DOM 1..8
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:Q69YU5"
FT TRANSMEM 9..25
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 26..67
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:Q69YU5"
SQ SEQUENCE 67 AA; 7540 MW; B970855318C5C92D CRC64;
MPGGVPWSAY LKMLSSSLLA MCAGAQVVHW YYRPDLTIPE IPPKPGELKT ELLGLKERRH
EPHVSQQ
