BX42_DROME
ID BX42_DROME Reviewed; 547 AA.
AC P39736; Q9W390;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Puff-specific protein Bx42;
GN Name=Bx42; ORFNames=CG8264;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Oregon-R; TISSUE=Embryo;
RX PubMed=1424996; DOI=10.1007/bf00352475;
RA Wieland C., Mann S., von Besser H., Saumweber H.;
RT "The Drosophila nuclear protein Bx42, which is found in many puffs on
RT polytene chromosomes, is highly charged.";
RL Chromosoma 101:517-525(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227 AND SER-235, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: May play a role in chromatin structure and function.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Two transcripts are detected of sizes 1.9 and 2.2
CC kb. Both are detected soon after fertilization and show relatively
CC constant expression during the first 2/3 of embryogenesis. In 0-3 hours
CC embryos, the smaller transcript is predominant and the levels of the
CC two transcripts are somewhat reduced at the later stages of
CC development, but they are found in approximately constant amounts
CC during larval, pupal and adult stages. The smaller transcript is
CC suspected to be a maternal transcript.
CC -!- SIMILARITY: Belongs to the SNW family. {ECO:0000305}.
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DR EMBL; X64536; CAA45834.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF46444.2; -; Genomic_DNA.
DR EMBL; AY113364; AAM29369.1; -; mRNA.
DR PIR; A56575; A56575.
DR RefSeq; NP_001259370.1; NM_001272441.2.
DR RefSeq; NP_511093.2; NM_078538.4.
DR AlphaFoldDB; P39736; -.
DR SMR; P39736; -.
DR BioGRID; 58295; 25.
DR DIP; DIP-17877N; -.
DR IntAct; P39736; 4.
DR MINT; P39736; -.
DR STRING; 7227.FBpp0305943; -.
DR iPTMnet; P39736; -.
DR PaxDb; P39736; -.
DR PRIDE; P39736; -.
DR DNASU; 31840; -.
DR EnsemblMetazoa; FBtr0071350; FBpp0071285; FBgn0004856.
DR EnsemblMetazoa; FBtr0333809; FBpp0305943; FBgn0004856.
DR GeneID; 31840; -.
DR KEGG; dme:Dmel_CG8264; -.
DR CTD; 31840; -.
DR FlyBase; FBgn0004856; Bx42.
DR VEuPathDB; VectorBase:FBgn0004856; -.
DR eggNOG; KOG2441; Eukaryota.
DR GeneTree; ENSGT00390000010423; -.
DR HOGENOM; CLU_006601_2_1_1; -.
DR InParanoid; P39736; -.
DR OMA; WRDSNTL; -.
DR OrthoDB; 1455730at2759; -.
DR PhylomeDB; P39736; -.
DR Reactome; R-DME-2173795; Downregulation of SMAD2/3:SMAD4 transcriptional activity.
DR Reactome; R-DME-350054; Notch-HLH transcription pathway.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR SignaLink; P39736; -.
DR BioGRID-ORCS; 31840; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31840; -.
DR PRO; PR:P39736; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0004856; Expressed in oviduct (Drosophila) and 25 other tissues.
DR ExpressionAtlas; P39736; baseline and differential.
DR Genevisible; P39736; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0000974; C:Prp19 complex; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001700; P:embryonic development via the syncytial blastoderm; IMP:FlyBase.
DR GO; GO:0035214; P:eye-antennal disc development; IMP:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IC:FlyBase.
DR InterPro; IPR017862; SKI-int_prot_SKIP.
DR InterPro; IPR004015; SKI-int_prot_SKIP_SNW-dom.
DR PANTHER; PTHR12096; PTHR12096; 1.
DR Pfam; PF02731; SKIP_SNW; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..547
FT /note="Puff-specific protein Bx42"
FT /id="PRO_0000084813"
FT REGION 177..343
FT /note="SNW"
FT REGION 333..398
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 486..547
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..398
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 522..541
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 227
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 235
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 547 AA; 61157 MW; 01399EA291C9D557 CRC64;
MSLSSLLPTP TNAIWDREDE RRLVARGAPK IGALVSAKIA APPYGQRKDW VPHTDADFGD
GGAFPEIHVA QYPLGLGAPG NVGKKSDALA VRLDDKGKVK YDAIARQGHG KDKIVYSSIS
QLLPAEVLAE DADELQRPDE ETVMETTEET RLALEKLTNQ KITSALPVRH AQKAGPAQYI
RYTPSQQGDT FNSGAKQRVI RMVEAQLDPM EPPKFRINKK IPRGPPSPPA PVLHSPSRKV
TVKEQKEWKI PPCISNWKNA KGYTIPLDKR LAADGRGLQQ VHINEKFAKM AEALYIADRK
AREAVEARSQ LEKKLAQKEK EKKEDMLRMM AQRAREERAG LRNPEAAEPS GSGATGSEVR
ERNDLRAERQ RERQRDRNLQ RAAPEKRSKL QKERERDISE QIALGLPAKS AGNGETLFDQ
RLFNTTKGMD SGYGDDEAYN VYDKPWRDSN TLGAHIYRPS KQADSDNYGG DLDAIVNTKR
FVPDKQFSGA SKEAAAGQRS GPVEFEKEED PFGLDQFLNM AKKAPKRAEE KNNERSSHSD
RKRSKRD
