BX8_MAIZE
ID BX8_MAIZE Reviewed; 459 AA.
AC Q8W2B7;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=DIMBOA UDP-glucosyltransferase BX8;
DE EC=2.4.1.202 {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
DE AltName: Full=2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase BX8;
DE AltName: Full=Protein BENZOXAZINLESS 8;
GN Name=Bx8;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION,
RP CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, AND
RP TISSUE SPECIFICITY.
RC STRAIN=cv. B73;
RX PubMed=11851909; DOI=10.1046/j.1365-313x.2001.01161.x;
RA von Rad U., Huttl R., Lottspeich F., Gierl A., Frey M.;
RT "Two glucosyltransferases are involved in detoxification of benzoxazinoids
RT in maize.";
RL Plant J. 28:633-642(2001).
RN [2]
RP CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND COFACTOR.
RC STRAIN=cv. CI31A;
RX PubMed=16666853; DOI=10.1104/pp.90.3.1071;
RA Bailey B.A., Larson R.L.;
RT "Hydroxamic acid glucosyltransferases from maize seedlings.";
RL Plant Physiol. 90:1071-1076(1989).
CC -!- FUNCTION: Glucosyltransferase involved in the last step of
CC benzoxazinoid glucoside biosynthesis. Catalyzes the glucosylation of
CC hydroxamic acids utilizing UDP-glucose as glucose doner, reducing the
CC toxicity of these natural insecticides for storage. Can use DIMBOA and
CC DIBOA as substrates, HMBOA (2-hydroxy-7-methoxy-2H-1,4-benzoxazin-
CC 3(4H)-one) and HBOA (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one) with a
CC lower efficiency, but not indole acetic acid or quercitin.
CC {ECO:0000269|PubMed:11851909}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=DIMBOA + UDP-alpha-D-glucose = DIMBOA beta-D-glucoside + H(+)
CC + UDP; Xref=Rhea:RHEA:15541, ChEBI:CHEBI:15378, ChEBI:CHEBI:18048,
CC ChEBI:CHEBI:37573, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885;
CC EC=2.4.1.202; Evidence={ECO:0000269|PubMed:11851909,
CC ECO:0000269|PubMed:16666853};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=DIBOA + UDP-alpha-D-glucose = DIBOA beta-D-glucoside + H(+) +
CC UDP; Xref=Rhea:RHEA:33955, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:63558, ChEBI:CHEBI:63670;
CC EC=2.4.1.202; Evidence={ECO:0000269|PubMed:11851909,
CC ECO:0000269|PubMed:16666853};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:16666853};
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000269|PubMed:16666853};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=81 uM for 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
CC (DIMBOA) {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
CC KM=61 uM for 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA)
CC {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
CC KM=81 uM for uridine 5'-diphosphoglucose (UDPG) with DIMBOA as
CC {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
CC Note=kcat is 22.7 sec(-1) for DIMBOA. kcat is 12.5 sec(-1) for DIBOA.
CC kcat is 22.6 sec(-1) for UDPG.;
CC pH dependence:
CC Optimum pH is 8.5. {ECO:0000269|PubMed:11851909,
CC ECO:0000269|PubMed:16666853};
CC Temperature dependence:
CC Optimum temperature is 45 degrees Celsius.
CC {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
CC -!- TISSUE SPECIFICITY: Expressed at the same levels in roots and shoots.
CC {ECO:0000269|PubMed:11851909}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in young seedlings up to 4 days
CC after imbibition. {ECO:0000269|PubMed:11851909}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AF331854; AAL57037.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8W2B7; -.
DR SMR; Q8W2B7; -.
DR STRING; 4577.GRMZM2G085054_P01; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; Q8W2B7; -.
DR PRIDE; Q8W2B7; -.
DR MaizeGDB; 9021865; -.
DR eggNOG; KOG1192; Eukaryota.
DR BioCyc; MetaCyc:MON-10602; -.
DR SABIO-RK; Q8W2B7; -.
DR Proteomes; UP000007305; Unplaced.
DR ExpressionAtlas; Q8W2B7; baseline and differential.
DR GO; GO:0047254; F:2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0080044; F:quercetin 7-O-glucosyltransferase activity; IBA:GO_Central.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycosyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..459
FT /note="DIMBOA UDP-glucosyltransferase BX8"
FT /id="PRO_0000415306"
FT BINDING 282
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 340..342
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 357..365
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 379..382
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 459 AA; 49470 MW; 0F5AE7AAA56AD3E7 CRC64;
MAASCGGRVV VFPFPFQGHF NPVMRLARAL HARGVGITVF HTAGARAPDP ADYPADYRFV
PVPVEVAPEL MASEDIAAIV TALNAACEAP FRDRLSALLS AADGEAGEAG GRVRCVLTDV
SWDAVLSAAR GLGVPALGVM TASAATFRVY MAYRTLVDKG YLPVREERKD DAVAELPPYR
VKDLLRHETC DLEEFADLLG RVIAAARLSS GLIFHTFPFI EAGTLGEIRD DMSVPVYAVA
PLNKLVPAAT ASLHGEVQAD RGCLRWLDAQ RARSVLYVSF GSMAAMDPHE FVELAWGLAD
AGRPFVWVVR PNLIRGFESG ALPDGVEDRV RGRGVVVSWA PQEEVLAHPA VGGFFTHCGW
NSTVEAVSEG VPMICHPRHG DQYGNARYVC HVWKVGTEVA GDQLERGEIK AAIDRLMGGS
EEGEGIRKRM NELKIAADKG IDESAGSDLT NLVHLINSY
