BX9_MAIZE
ID BX9_MAIZE Reviewed; 462 AA.
AC B4G072; Q8W2B6;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=DIMBOA UDP-glucosyltransferase BX9;
DE EC=2.4.1.202 {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
DE AltName: Full=2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase BX9;
DE AltName: Full=Protein BENZOXAZINLESS 9;
GN Name=BX9;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION,
RP CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, AND
RP TISSUE SPECIFICITY.
RC STRAIN=cv. CI31A;
RX PubMed=11851909; DOI=10.1046/j.1365-313x.2001.01161.x;
RA von Rad U., Huttl R., Lottspeich F., Gierl A., Frey M.;
RT "Two glucosyltransferases are involved in detoxification of benzoxazinoids
RT in maize.";
RL Plant J. 28:633-642(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. B73;
RA Yu Y., Currie J., Lomeli R., Angelova A., Collura K., Wissotski M.,
RA Campos D., Kudrna D., Golser W., Ashely E., Haller K., Descour A.,
RA Fernandes J., Zuccolo A., Soderlund C., Walbot V.;
RT "Maize full-length cDNA project.";
RL Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND COFACTOR.
RC STRAIN=cv. CI31A;
RX PubMed=16666853; DOI=10.1104/pp.90.3.1071;
RA Bailey B.A., Larson R.L.;
RT "Hydroxamic acid glucosyltransferases from maize seedlings.";
RL Plant Physiol. 90:1071-1076(1989).
CC -!- FUNCTION: Glucosyltransferase involved in the last step of
CC benzoxazinoid glucoside biosynthesis. Catalyzes the glucosylation of
CC hydroxamic acids utilizing UDP-glucose as glucose doner, reducing the
CC toxicity of these natural insecticides for storage. Can use DIMBOA and
CC DIBOA as substrates, HMBOA (2-hydroxy-7-methoxy-2H-1,4-benzoxazin-
CC 3(4H)-one) and HBOA (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one) with a
CC lower efficiency, but not indole acetic acid or quercitin.
CC {ECO:0000269|PubMed:11851909}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=DIMBOA + UDP-alpha-D-glucose = DIMBOA beta-D-glucoside + H(+)
CC + UDP; Xref=Rhea:RHEA:15541, ChEBI:CHEBI:15378, ChEBI:CHEBI:18048,
CC ChEBI:CHEBI:37573, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885;
CC EC=2.4.1.202; Evidence={ECO:0000269|PubMed:11851909,
CC ECO:0000269|PubMed:16666853};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=DIBOA + UDP-alpha-D-glucose = DIBOA beta-D-glucoside + H(+) +
CC UDP; Xref=Rhea:RHEA:33955, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:63558, ChEBI:CHEBI:63670;
CC EC=2.4.1.202; Evidence={ECO:0000269|PubMed:11851909,
CC ECO:0000269|PubMed:16666853};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:16666853};
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000269|PubMed:16666853};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=71 uM for 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
CC (DIMBOA) {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
CC KM=1300 uM for 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA)
CC {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
CC KM=96 uM for uridine 5'-diphosphoglucose (UDPG)
CC {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
CC Note=kcat is 11.6 sec(-1) for DIMBOA. kcat is 12.5 sec(-1) for DIBOA.
CC kcat is 22.6 sec(-1) for UDPG.;
CC pH dependence:
CC Optimum pH is 8.5. {ECO:0000269|PubMed:11851909,
CC ECO:0000269|PubMed:16666853};
CC Temperature dependence:
CC Optimum temperature is 45 degrees Celsius.
CC {ECO:0000269|PubMed:11851909, ECO:0000269|PubMed:16666853};
CC -!- TISSUE SPECIFICITY: Expressed at the same levels in roots and shoots.
CC {ECO:0000269|PubMed:11851909}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in young seedlings up to 4 days
CC after imbibition. {ECO:0000269|PubMed:11851909}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AF331855; AAL57038.1; -; Genomic_DNA.
DR EMBL; BT042760; ACF87765.1; -; mRNA.
DR RefSeq; NP_001142152.1; NM_001148680.1.
DR AlphaFoldDB; B4G072; -.
DR SMR; B4G072; -.
DR STRING; 4577.GRMZM2G161335_P01; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; B4G072; -.
DR PRIDE; B4G072; -.
DR EnsemblPlants; Zm00001eb033030_T001; Zm00001eb033030_P001; Zm00001eb033030.
DR GeneID; 100274317; -.
DR Gramene; Zm00001eb033030_T001; Zm00001eb033030_P001; Zm00001eb033030.
DR KEGG; zma:100274317; -.
DR MaizeGDB; 9021865; -.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_0_0_1; -.
DR OMA; IVVAWAP; -.
DR OrthoDB; 508327at2759; -.
DR BioCyc; MetaCyc:MON-10603; -.
DR SABIO-RK; B4G072; -.
DR Proteomes; UP000007305; Chromosome 1.
DR ExpressionAtlas; B4G072; baseline and differential.
DR Genevisible; B4G072; ZM.
DR GO; GO:0047254; F:2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0080044; F:quercetin 7-O-glucosyltransferase activity; IBA:GO_Central.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR Pfam; PF00201; UDPGT; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycosyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..462
FT /note="DIMBOA UDP-glucosyltransferase BX9"
FT /id="PRO_0000415307"
FT BINDING 278
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 336..338
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 353..361
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 375..378
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT CONFLICT 6
FT /note="T -> TGA (in Ref. 1; AAL57038)"
FT /evidence="ECO:0000305"
FT CONFLICT 176
FT /note="L -> R (in Ref. 1; AAL57038)"
FT /evidence="ECO:0000305"
FT CONFLICT 334
FT /note="A -> T (in Ref. 1; AAL57038)"
FT /evidence="ECO:0000305"
FT CONFLICT 446
FT /note="A -> T (in Ref. 1; AAL57038)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 462 AA; 50017 MW; 2314E86FA7AF9A6F CRC64;
MASSRTGAGA GGRVVVFPFP FQGHFNPVMR LARALHARGL AITVFHSGAL DPADYPADYR
FVPVTVEADP KLLASEDIAA IVTTLNASCD APFRARLSAL LAAEGRDSVR CVFTDVSWNA
VLTASSDLGV PALGMMTASA ASLRDYMAYR TLIDKGYLPV KEERKEDPVP ELPPYLVKDL
LRVDTSDLEE FAELLARTVT AARRASGLIF NTFPLIETDT LAEIHKALSV PVFAVAPLNK
LVPTATASLH GVVQADRGCL QWLDTQQPGS VLYVSFGSMA AMDPHEFVEL AWGLADSKRP
FVWVVRPNLI RGFESGALPD GVEDEVRGRG IVVAWAPQEE VLAHPAVGGF LTHNGWNSTV
EAISEGVPMV CCPRHGDQFG NMRYVCDVWK VGTELVGEQL ERGQVKAAID RLFGTKEGEE
IKERMKEFKI AAAKGIGIGV DVDETASPRT DLTDLVDLIK SF
