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TRMD_HAEIN
ID   TRMD_HAEIN              Reviewed;         246 AA.
AC   P43912;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 142.
DE   RecName: Full=tRNA (guanine-N(1)-)-methyltransferase;
DE            EC=2.1.1.228;
DE   AltName: Full=M1G-methyltransferase;
DE   AltName: Full=tRNA [GM37] methyltransferase;
GN   Name=trmD; OrderedLocusNames=HI_0202;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, AND
RP   SUBUNIT.
RX   PubMed=12773376; DOI=10.1093/emboj/cdg269;
RA   Ahn H.J., Kim H.-W., Yoon H.-J., Lee B.I., Suh S.W., Yang J.K.;
RT   "Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA
RT   recognition.";
RL   EMBO J. 22:2593-2603(2003).
CC   -!- FUNCTION: Specifically methylates guanosine-37 in various tRNAs.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC         methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:36899, Rhea:RHEA-COMP:10145, Rhea:RHEA-COMP:10147,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.228;
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12773376}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase TrmD family.
CC       {ECO:0000305}.
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DR   EMBL; L42023; AAC21871.1; -; Genomic_DNA.
DR   PIR; C64054; C64054.
DR   RefSeq; NP_438371.1; NC_000907.1.
DR   RefSeq; WP_005661387.1; NC_000907.1.
DR   PDB; 1UAJ; X-ray; 1.85 A; A=1-246.
DR   PDB; 1UAK; X-ray; 2.05 A; A=1-246.
DR   PDB; 1UAL; X-ray; 1.80 A; A=1-246.
DR   PDB; 1UAM; X-ray; 2.20 A; A=1-246.
DR   PDB; 3AXZ; X-ray; 2.25 A; A=1-246.
DR   PDB; 4MCB; X-ray; 1.94 A; A/B=1-246.
DR   PDB; 4MCC; X-ray; 1.95 A; A/B=1-246.
DR   PDB; 4MCD; X-ray; 1.55 A; A=1-246.
DR   PDB; 4YPW; X-ray; 2.31 A; A=1-246.
DR   PDB; 4YPX; X-ray; 1.89 A; A=1-246.
DR   PDB; 4YPY; X-ray; 1.90 A; A=1-246.
DR   PDB; 4YPZ; X-ray; 1.84 A; A=1-246.
DR   PDB; 4YQ0; X-ray; 1.76 A; A=1-246.
DR   PDB; 4YQ1; X-ray; 2.00 A; A=1-246.
DR   PDB; 4YQ2; X-ray; 2.65 A; A=1-246.
DR   PDB; 4YQ3; X-ray; 2.49 A; A=1-246.
DR   PDB; 4YQ4; X-ray; 1.89 A; A=1-246.
DR   PDB; 4YQ5; X-ray; 1.76 A; A=1-246.
DR   PDB; 4YQ6; X-ray; 1.90 A; A=1-246.
DR   PDB; 4YQ7; X-ray; 1.80 A; A=1-246.
DR   PDB; 4YQ8; X-ray; 1.94 A; A=1-246.
DR   PDB; 4YQ9; X-ray; 1.64 A; A=1-246.
DR   PDB; 4YQA; X-ray; 1.55 A; A=1-246.
DR   PDB; 4YQB; X-ray; 2.10 A; A=1-246.
DR   PDB; 4YQC; X-ray; 1.89 A; A=1-246.
DR   PDB; 4YQD; X-ray; 1.45 A; A=1-246.
DR   PDB; 4YQG; X-ray; 1.86 A; A=1-246.
DR   PDB; 4YQI; X-ray; 1.92 A; A=1-246.
DR   PDB; 4YQJ; X-ray; 1.94 A; A=1-246.
DR   PDB; 4YQK; X-ray; 1.83 A; A=1-246.
DR   PDB; 4YQL; X-ray; 2.40 A; A=1-246.
DR   PDB; 4YQN; X-ray; 2.20 A; A=1-246.
DR   PDB; 4YQO; X-ray; 1.68 A; A=1-246.
DR   PDB; 4YQP; X-ray; 2.60 A; A=1-246.
DR   PDB; 4YQQ; X-ray; 1.78 A; A=1-246.
DR   PDB; 4YQR; X-ray; 1.70 A; A=1-246.
DR   PDB; 4YQS; X-ray; 1.90 A; A=1-246.
DR   PDB; 4YQT; X-ray; 1.60 A; A=1-246.
DR   PDB; 4YVG; X-ray; 1.55 A; A=1-246.
DR   PDB; 4YVH; X-ray; 1.60 A; A=1-246.
DR   PDB; 4YVI; X-ray; 3.01 A; A/B=1-246.
DR   PDB; 4YVJ; X-ray; 2.90 A; A/B=1-246.
DR   PDB; 4YVK; X-ray; 3.00 A; A/B=1-246.
DR   PDB; 5D9F; X-ray; 1.91 A; A=1-246.
DR   PDBsum; 1UAJ; -.
DR   PDBsum; 1UAK; -.
DR   PDBsum; 1UAL; -.
DR   PDBsum; 1UAM; -.
DR   PDBsum; 3AXZ; -.
DR   PDBsum; 4MCB; -.
DR   PDBsum; 4MCC; -.
DR   PDBsum; 4MCD; -.
DR   PDBsum; 4YPW; -.
DR   PDBsum; 4YPX; -.
DR   PDBsum; 4YPY; -.
DR   PDBsum; 4YPZ; -.
DR   PDBsum; 4YQ0; -.
DR   PDBsum; 4YQ1; -.
DR   PDBsum; 4YQ2; -.
DR   PDBsum; 4YQ3; -.
DR   PDBsum; 4YQ4; -.
DR   PDBsum; 4YQ5; -.
DR   PDBsum; 4YQ6; -.
DR   PDBsum; 4YQ7; -.
DR   PDBsum; 4YQ8; -.
DR   PDBsum; 4YQ9; -.
DR   PDBsum; 4YQA; -.
DR   PDBsum; 4YQB; -.
DR   PDBsum; 4YQC; -.
DR   PDBsum; 4YQD; -.
DR   PDBsum; 4YQG; -.
DR   PDBsum; 4YQI; -.
DR   PDBsum; 4YQJ; -.
DR   PDBsum; 4YQK; -.
DR   PDBsum; 4YQL; -.
DR   PDBsum; 4YQN; -.
DR   PDBsum; 4YQO; -.
DR   PDBsum; 4YQP; -.
DR   PDBsum; 4YQQ; -.
DR   PDBsum; 4YQR; -.
DR   PDBsum; 4YQS; -.
DR   PDBsum; 4YQT; -.
DR   PDBsum; 4YVG; -.
DR   PDBsum; 4YVH; -.
DR   PDBsum; 4YVI; -.
DR   PDBsum; 4YVJ; -.
DR   PDBsum; 4YVK; -.
DR   PDBsum; 5D9F; -.
DR   AlphaFoldDB; P43912; -.
DR   SMR; P43912; -.
DR   STRING; 71421.HI_0202; -.
DR   BindingDB; P43912; -.
DR   DrugBank; DB01752; S-adenosyl-L-homocysteine.
DR   EnsemblBacteria; AAC21871; AAC21871; HI_0202.
DR   KEGG; hin:HI_0202; -.
DR   PATRIC; fig|71421.8.peg.207; -.
DR   eggNOG; COG0336; Bacteria.
DR   HOGENOM; CLU_047363_0_1_6; -.
DR   OMA; ILCGHYK; -.
DR   PhylomeDB; P43912; -.
DR   BioCyc; HINF71421:G1GJ1-213-MON; -.
DR   BRENDA; 2.1.1.228; 2529.
DR   EvolutionaryTrace; P43912; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0052906; F:tRNA (guanine(37)-N(1))-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0002939; P:tRNA N1-guanine methylation; IBA:GO_Central.
DR   CDD; cd18080; TrmD-like; 1.
DR   Gene3D; 1.10.1270.20; -; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   HAMAP; MF_00605; TrmD; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR002649; tRNA_m1G_MeTrfase_bac.
DR   InterPro; IPR023148; tRNA_m1G_MeTrfase_C_sf.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR   PANTHER; PTHR46417; PTHR46417; 1.
DR   Pfam; PF01746; tRNA_m1G_MT; 1.
DR   PIRSF; PIRSF000386; tRNA_mtase; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00088; trmD; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing.
FT   CHAIN           1..246
FT                   /note="tRNA (guanine-N(1)-)-methyltransferase"
FT                   /id="PRO_0000060385"
FT   ACT_SITE        169
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   BINDING         86
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   BINDING         113
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   BINDING         133..138
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   STRAND          1..6
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           10..13
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           14..17
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           20..27
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   STRAND          30..36
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           38..41
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           64..78
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   STRAND          83..88
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   STRAND          91..93
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           96..102
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   STRAND          106..111
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           120..126
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   STRAND          128..136
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   STRAND          139..141
FT                   /evidence="ECO:0007829|PDB:4YVH"
FT   HELIX           142..153
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   TURN            157..159
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           164..166
FT                   /evidence="ECO:0007829|PDB:4YVJ"
FT   TURN            170..172
FT                   /evidence="ECO:0007829|PDB:4YVG"
FT   STRAND          173..175
FT                   /evidence="ECO:0007829|PDB:4YVG"
FT   STRAND          185..187
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           194..197
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           201..219
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           221..225
FT                   /evidence="ECO:0007829|PDB:4YQD"
FT   HELIX           231..245
FT                   /evidence="ECO:0007829|PDB:4YQD"
SQ   SEQUENCE   246 AA;  27543 MW;  DEEF238159B1003D CRC64;
     MWIGVISLFP EMFKAITEFG VTGRAVKHNL LKVECWNPRD FTFDKHKTVD DRPYGGGPGM
     LMMVQPLRDA IHTAKAAAGE GAKVIYLSPQ GRKLDQGGVT ELAQNQKLIL VCGRYEGIDE
     RLIQTEIDEE WSIGDYVLTG GELPAMTLID AVARFIPGVL GKQASAEEDS FADGLLDCPH
     YTRPEVLEGL TVPPVLMSGH HEEIRKWRLK QSLQRTWLRR PELLEGLALT DEQRKLLKEA
     QAEHNS
 
 
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