ID   BXE1_BOMMO              Reviewed;          98 AA.
AC   P21808; O18329;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 4.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Bombyxin E-1;
DE            Short=BBX-E1;
DE   AltName: Full=4K-prothoracicotropic hormone IV;
DE            Short=4K-PTTH-IV;
DE   AltName: Full=Bombyxin IV;
DE   Contains:
DE     RecName: Full=Bombyxin E-1 B chain;
DE   Contains:
DE     RecName: Full=Bombyxin E-1 A chain;
DE   Flags: Precursor;
GN   Name=BBXE1;
OS   Bombyx mori (Silk moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Bombycidae; Bombycinae; Bombyx.
OX   NCBI_TaxID=7091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Kinshu X Showa;
RX   PubMed=9253178; DOI=10.1016/s0305-0491(96)00332-x;
RA   Tsuzuki S., Masuta T., Furuno M., Sakurai S., Iwami M.;
RT   "Structure and expression of bombyxin E1 gene: a novel family gene that
RT   encodes bombyxin-IV, an insect insulin-related neurosecretory peptide.";
RL   Comp. Biochem. Physiol. 117B:409-416(1997).
RN   [2]
RP   PROTEIN SEQUENCE OF 48-67 AND 79-98, AND PYROGLUTAMATE FORMATION AT GLN-20.
RA   Maruyama K., Hietter H., Nagasawa H., Isogai A., Tamura S., Suzuki A.,
RA   Ishizaki H.;
RT   "Isolation and primary structure of bombyxin-IV, a novel molecular species
RT   of bombyxin from the silkworm, Bombyx mori.";
RL   Agric. Biol. Chem. 52:3035-3041(1988).
RN   [3]
RP   DISULFIDE BONDS.
RX   PubMed=1515030; DOI=10.1007/bf01025087;
RA   Maruyama K., Nagasawa H., Isogai A., Ishizaki H., Suzuki A.;
RT   "Determination of disulfide bond arrangement in bombyxin-IV, an insulin
RT   superfamily peptide from the silkworm, Bombyx mori, by combination of
RT   thermolysin digestion of natural peptide and selective synthesis of
RT   disulfide bond isomers.";
RL   J. Protein Chem. 11:13-20(1992).
CC   -!- FUNCTION: PTTH is a brain peptide responsible for activation of
CC       prothoracic glands to produce ecdysone in insects.
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MISCELLANEOUS: Silk worm has two kinds of PTTH: 4K-PTTH and 22K-PTTH;
CC       there are four forms of 4K-PTTH.
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR   EMBL; D78138; BAA22159.1; -; Genomic_DNA.
DR   PIR; JT0410; JT0410.
DR   PIR; JT0412; JT0412.
DR   RefSeq; NP_001119733.1; NM_001126261.1.
DR   AlphaFoldDB; P21808; -.
DR   STRING; 7091.BGIBMGA012294-TA; -.
DR   GeneID; 100146109; -.
DR   KEGG; bmor:100146109; -.
DR   CTD; 100146109; -.
DR   eggNOG; ENOG502SESX; Eukaryota.
DR   HOGENOM; CLU_125164_2_0_1; -.
DR   InParanoid; P21808; -.
DR   OrthoDB; 1644517at2759; -.
DR   Proteomes; UP000005204; Unassembled WGS sequence.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0008083; F:growth factor activity; IEA:InterPro.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   InterPro; IPR017097; Bombyxin.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR022353; Insulin_CS.
DR   InterPro; IPR022352; Insulin_family.
DR   Pfam; PF00049; Insulin; 1.
DR   PIRSF; PIRSF037038; Bombyxin; 1.
DR   PRINTS; PR00276; INSULINFAMLY.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; SSF56994; 1.
DR   PROSITE; PS00262; INSULIN; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Hormone; Pyrrolidone carboxylic acid; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..19
FT   PEPTIDE         20..47
FT                   /note="Bombyxin E-1 B chain"
FT                   /evidence="ECO:0000269|Ref.2"
FT                   /id="PRO_0000016034"
FT   PROPEP          50..75
FT                   /note="C peptide like"
FT                   /id="PRO_0000016035"
FT   PEPTIDE         79..98
FT                   /note="Bombyxin E-1 A chain"
FT                   /id="PRO_0000016036"
FT   MOD_RES         20
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|Ref.2"
FT   DISULFID        29..85
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000269|PubMed:1515030"
FT   DISULFID        41..98
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000269|PubMed:1515030"
FT   DISULFID        84..89
FT                   /evidence="ECO:0000269|PubMed:1515030"
SQ   SEQUENCE   98 AA;  10987 MW;  8890301EF39AB093 CRC64;
     MNRPVFLVLL LTGFLCIAAQ EANVAHHYCG RHLANTLADL CWDTSVEKRS ESSLASYSSR
     GWPWLPTPNF NKRAIKKRGV VDECCIQPCT LDVLATYC