ID   BXEN_CLOBO              Reviewed;        1162 AA.
AC   P46082;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Non-toxic nonhemagglutinin type E;
DE            Short=NTNHA;
DE   AltName: Full=Botulinum neurotoxin type E non-toxic component;
GN   Name=ent-120;
OS   Clostridium botulinum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1491;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Mashike / Type E;
RX   PubMed=8450310; DOI=10.1099/00221287-139-1-79;
RA   Fujii N., Kimura K., Yokosawa N., Yashiki T., Tsuzuki K., Oguma K.;
RT   "The complete nucleotide sequence of the gene encoding the nontoxic
RT   component of Clostridium botulinum type E progenitor toxin.";
RL   J. Gen. Microbiol. 139:79-86(1993).
CC   -!- FUNCTION: Assembles with botulinum neurotoxin type E (BoNT/E) and
CC       protects it against pH-mediated inactivation or protease activity at pH
CC       2.6 (the pH of the animal gastrointestinal tract) but not at pH 6.0 (By
CC       similarity). The non-toxic component is necessary to maintain toxicity.
CC       {ECO:0000250|UniProtKB:Q45914}.
CC   -!- SUBUNIT: Forms a highly interlocked heterodimer with botulinum
CC       neurotoxin type E at pH 6.0 but not at pH 7.5 (By similarity).
CC       {ECO:0000250|UniProtKB:Q45914}.
CC   -!- SIMILARITY: Belongs to the botulism non-toxic nonhemagglutinin family.
CC       {ECO:0000305}.
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DR   EMBL; D12697; BAA02194.1; -; Genomic_DNA.
DR   PIR; A47708; A47708.
DR   AlphaFoldDB; P46082; -.
DR   SMR; P46082; -.
DR   PRIDE; P46082; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0046929; P:negative regulation of neurotransmitter secretion; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   Gene3D; 1.20.1120.10; -; 1.
DR   InterPro; IPR000395; Bot/tetX_LC.
DR   InterPro; IPR036248; Clostridium_toxin_transloc.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR013677; Nontoxic_nonhemagglutn_C.
DR   InterPro; IPR012928; Toxin_rcpt-bd_N.
DR   Pfam; PF08470; NTNH_C; 1.
DR   Pfam; PF01742; Peptidase_M27; 1.
DR   Pfam; PF07953; Toxin_R_bind_N; 1.
DR   PRINTS; PR00760; BONTOXILYSIN.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF58091; SSF58091; 1.
PE   3: Inferred from homology;
KW   Virulence.
FT   CHAIN           1..1162
FT                   /note="Non-toxic nonhemagglutinin type E"
FT                   /id="PRO_0000065029"
SQ   SEQUENCE   1162 AA;  136856 MW;  96468EDDDAE0F39D CRC64;
     MKINGNLNID SPVDNKNVAI VRSRNQMFFK AFQVAPNIWI VPERYYGESL KINEDQKFDG
     GIYDSNFLST NNEKDDFLQA TIKLLQRINN NVVGAKLLSL ISTAIPFPYE NNTEDYRQTN
     YLSSKNNEHY YTANLVIFGP GSNIIKNNVI YYKKEYAESG MGTMLEIWFQ PFLTHKYDEF
     YVDPALELIK CLIKSLYYLY GIKPNDNLNI PYRLRNEFNS LEYSELNMID FLISGGIDYK
     LLNTNPYWFI DKYFIDTSKN FEKYKNDYEI KIKNNNYIAN SIKLYLEQKF KINVKDIWEL
     NLSYFSKEFQ IMMPERYNNA LNHYYRKEFY VIDYFKNYNI NGFKNGQIKT KLPLSKYNKE
     IINKPELIVN LINQNNTVLM KSNIYGDGLK GTVDNFYSNY IIPYNLNYEH SINYFYLDNV
     NIEEIEKIPP INDEDIYPYR KNADTFIPVY NITKAKEINT TTPLPVNYLQ AQMIDSNDIN
     LSSDFLKVIS SKGSLVYSFL NNTMDYLEFI KYDKPIDTDK KYYKWLKAIF RNYSLDITET
     QEISNQFGDT KIIPWIGRAL NILNTNNSFV EEFKNLGPIS LINKKENITI PKIKIDEIPS
     SMLNFSFKDL SENLFNIYCK NNFYLKKIYY NFLDQWWTQY YSQYFDLICM ASKSVLAQEK
     LIKKLIQKQL RYLMENSNIS STNLILINLT TTNTLRDISN QSQIAINNID KFFNNAAMCV
     FENNIYPKFT SFMEQCIKNI NKSTKEFILK CTNINETEKS HLIMQNSFSN LDFDFLDIQN
     MKNLFNLYTE LLIKEQTSPY ELSLYAFQEQ DNNVIGDTSG KNTLVEYPKD IGLVYGINNN
     AIHLTGANQN IKFTNDYFEN GLTNNFSIYF WLRNLKQNTI KSKLIGSKED NCGWEIYFEN
     DGLVFNIIDS NGNEKNIYLS NISNKSWHYI VISINRLKDQ LLIFIDNILV ANEDIKEILN
     IYSSDIISLL SDNNNVYIEG LSVLNKTINS NEILTDYFSD LNNSYIRNFD EEILQYNRTY
     ELFNYVFPEI AINKIEQNNN IYLSINNENN LNFKPLKFKL LNTNPNKQYV QKWDEVIFSV
     LDGTEKYLDI STTNNRIQLV DNKNNAQIFI INNDIFISNC LTLTYNNVNV YLSIKNQDYN
     WVICDLNHDI PKKSYLWILK NI