ID   BXEN_CLOBU              Reviewed;        1162 AA.
AC   Q06366;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Non-toxic nonhemagglutinin type E;
DE            Short=NTNHA;
DE   AltName: Full=Botulinum neurotoxin type E non-toxic component;
OS   Clostridium butyricum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1492;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BL6340;
RX   PubMed=8355622; DOI=10.1111/j.1348-0421.1993.tb03227.x;
RA   Fujii N., Kimura K., Yokosawa N., Oguma K., Yashiki T., Takeshi K.,
RA   Ohyama T., Isogai E., Isogai H.;
RT   "Similarity in nucleotide sequence of the gene encoding nontoxic component
RT   of botulinum toxin produced by toxigenic Clostridium butyricum strain
RT   BL6340 and Clostridium botulinum type E strain Mashike.";
RL   Microbiol. Immunol. 37:395-398(1993).
CC   -!- FUNCTION: Assembles with botulinum neurotoxin type E (BoNT/E) and
CC       protects it against pH-mediated inactivation or protease activity at pH
CC       2.6 (the pH of the animal gastrointestinal tract) but not at pH 6.0 (By
CC       similarity). The non-toxic component is necessary to maintain toxicity.
CC       {ECO:0000250|UniProtKB:Q45914}.
CC   -!- SUBUNIT: Forms a highly interlocked heterodimer with botulinum
CC       neurotoxin type E at pH 6.0 but not at pH 7.5 (By similarity).
CC       {ECO:0000250|UniProtKB:Q45914}.
CC   -!- SIMILARITY: Belongs to the botulism non-toxic nonhemagglutinin family.
CC       {ECO:0000305}.
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DR   EMBL; D12739; BAA02231.1; -; Genomic_DNA.
DR   PIR; I40817; I40817.
DR   AlphaFoldDB; Q06366; -.
DR   SMR; Q06366; -.
DR   PRIDE; Q06366; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0046929; P:negative regulation of neurotransmitter secretion; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   Gene3D; 1.20.1120.10; -; 1.
DR   InterPro; IPR000395; Bot/tetX_LC.
DR   InterPro; IPR036248; Clostridium_toxin_transloc.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR013677; Nontoxic_nonhemagglutn_C.
DR   InterPro; IPR012928; Toxin_rcpt-bd_N.
DR   Pfam; PF08470; NTNH_C; 1.
DR   Pfam; PF01742; Peptidase_M27; 1.
DR   Pfam; PF07953; Toxin_R_bind_N; 1.
DR   PRINTS; PR00760; BONTOXILYSIN.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF58091; SSF58091; 1.
PE   3: Inferred from homology;
KW   Virulence.
FT   CHAIN           1..1162
FT                   /note="Non-toxic nonhemagglutinin type E"
FT                   /id="PRO_0000065030"
SQ   SEQUENCE   1162 AA;  136830 MW;  C86E9BE932DA78E4 CRC64;
     MKINGNLNID SPVDNKNVAI VRSRNQMFFK AFQVAPNIWI APERYYGESL KINEDQKSDG
     GIYDSNFLST NNEKDEFLQA TIKLLQRINN NVVGAKLLSL ISTAIPFPYE NNTEDYRQTN
     YLSSKNNEHY YTANLVIFGP GSNIIKNNVI YYKKEYAENG MGTMLEIWFQ PFLTHKYDEF
     YVDPALELIK CLIKSLYYLY GIKPNDNLNI PYRLRNEFNS LEYSELDMID FLISGGIDYK
     LLNTNPYWFI DKYFIDTSKN FEKYKNDYEI KIKNNNYIAN SIKLYLEQKF KINVKDIWEL
     NLSYFSKEFQ IMMPERYNNA LNHYYRKEFY VIDYFKNYNI NGFKNGQIKT KLPLSKYNKE
     IINKPELIVN LINQNNTVLM KSNFYGDGLK GNVDNFYSNY IIPYNLNYEH SINYSYLDNV
     NIEEIEKIPP INDEDIYPYR KNADTFIPVY NITKAKEINT TTPLPVNYLQ AQMIDSNDIN
     LSSDFLKVIS SKGSLVYSFL NNTMDYLEFI KYDKPIDTDK KYYKWLKAIF RNYSLDITET
     QEISNQFGDT KIIPWIGRAL NILNTNNSFV EEFKNLGPIF LINKKENITI PKIKIDEIPS
     SMLNFSFKDL SENLFNIYCK NNFYLKKIYY NFLDQWWTQY YSQYFDLICM ASKSVLAQEK
     LIKKLIQKQL RYLMENSNIS STNLILINLT TTNTLRDISN QSQIAINNID KFFNNAAMCV
     FENNIYPKFT SFMEQCIKNI NKSTKEFILK CTNINETEKS HLIMQNSFSN LDFDFLDIQN
     MKNLFNLYTE LLIKEQTSPY ELSLYAFQEQ DNNVIGDTSG KNTLVEYPKD IGLVYGINNN
     AIHLTGANQN IKFTNDYFEN GLTNNFSIYF WLRNLNQNTI KSKLIGSKED NCGWEIYFEN
     NGLVFNIIDS NGNEKNIYLS NISNKSWHYI VISINRLKDQ LLIFIDNILV ANEDIKEILN
     IYSSDIISLL SDNNNVYIEG LSVLNKTINS NEILTDYFSD LNNSYIRNFD EEILQYNRTY
     ELFNYVFPEI AINKIEQNNN IYLSNNNENS LNFKPLKFKL LNTNPNKQYV QKWDEVIFSV
     LDGTEKYLDI SIDNNRIQLV DNKNNAKTFI INNDIFISNC LTLTYNNVNV YLSIKNQDYN
     WVICDLNHDI PKKSYLWILK NI