BXL2_ARATH
ID BXL2_ARATH Reviewed; 768 AA.
AC Q94KD8; Q9FWY2;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Probable beta-D-xylosidase 2;
DE Short=AtBXL2;
DE EC=3.2.1.-;
DE Flags: Precursor;
GN Name=BXL2; OrderedLocusNames=At1g02640; ORFNames=T14P4.11, T14P4.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION.
RX PubMed=12609041; DOI=10.1046/j.1365-313x.2003.01654.x;
RA Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C.,
RA Joseleau J.-P., Jouanin L.;
RT "AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-
RT xylosidase gene, is involved in secondary cell wall metabolism and plant
RT development.";
RL Plant J. 33:677-690(2003).
RN [5]
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19458117; DOI=10.1104/pp.109.138388;
RA Arsovski A.A., Popma T.M., Haughn G.W., Carpita N.C., McCann M.C.,
RA Western T.L.;
RT "AtBXL1 encodes a bifunctional beta-D-xylosidase/alpha-L-
RT arabinofuranosidase required for pectic arabinan modification in
RT arabidopsis mucilage secretory cells.";
RL Plant Physiol. 150:1219-1234(2009).
CC -!- FUNCTION: May be involved in remodeling of xylans during vascular
CC development. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, stems, seedlings, roots,
CC inflorescences and siliques. {ECO:0000269|PubMed:19458117}.
CC -!- DISRUPTION PHENOTYPE: Partially redundant with BXL1. Bxl1 and bxl2
CC double mutants have shortened siliques and curled leaf edges.
CC {ECO:0000269|PubMed:19458117}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG10624.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC022521; AAG10624.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE27453.1; -; Genomic_DNA.
DR EMBL; AF367266; AAK56255.1; -; mRNA.
DR EMBL; AY143952; AAN28891.1; -; mRNA.
DR PIR; D86156; D86156.
DR RefSeq; NP_563659.1; NM_100144.3.
DR AlphaFoldDB; Q94KD8; -.
DR SMR; Q94KD8; -.
DR BioGRID; 23180; 1.
DR STRING; 3702.AT1G02640.1; -.
DR CAZy; GH3; Glycoside Hydrolase Family 3.
DR PaxDb; Q94KD8; -.
DR PRIDE; Q94KD8; -.
DR ProteomicsDB; 240294; -.
DR EnsemblPlants; AT1G02640.1; AT1G02640.1; AT1G02640.
DR GeneID; 837940; -.
DR Gramene; AT1G02640.1; AT1G02640.1; AT1G02640.
DR KEGG; ath:AT1G02640; -.
DR Araport; AT1G02640; -.
DR TAIR; locus:2196060; AT1G02640.
DR eggNOG; ENOG502QQ55; Eukaryota.
DR HOGENOM; CLU_004542_5_3_1; -.
DR InParanoid; Q94KD8; -.
DR OMA; VEPAYEF; -.
DR OrthoDB; 321444at2759; -.
DR PhylomeDB; Q94KD8; -.
DR BioCyc; ARA:AT1G02640-MON; -.
DR PRO; PR:Q94KD8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94KD8; baseline and differential.
DR Genevisible; Q94KD8; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0046556; F:alpha-L-arabinofuranosidase activity; IBA:GO_Central.
DR GO; GO:0009044; F:xylan 1,4-beta-xylosidase activity; IBA:GO_Central.
DR GO; GO:0031222; P:arabinan catabolic process; IBA:GO_Central.
DR GO; GO:0045493; P:xylan catabolic process; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.20.20.300; -; 1.
DR Gene3D; 3.40.50.1700; -; 1.
DR InterPro; IPR044993; BXL.
DR InterPro; IPR026891; Fn3-like.
DR InterPro; IPR002772; Glyco_hydro_3_C.
DR InterPro; IPR036881; Glyco_hydro_3_C_sf.
DR InterPro; IPR001764; Glyco_hydro_3_N.
DR InterPro; IPR036962; Glyco_hydro_3_N_sf.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR42721; PTHR42721; 1.
DR Pfam; PF14310; Fn3-like; 1.
DR Pfam; PF00933; Glyco_hydro_3; 1.
DR Pfam; PF01915; Glyco_hydro_3_C; 1.
DR PRINTS; PR00133; GLHYDRLASE3.
DR SMART; SM01217; Fn3_like; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF52279; SSF52279; 1.
PE 2: Evidence at transcript level;
KW Extracellular matrix; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..768
FT /note="Probable beta-D-xylosidase 2"
FT /id="PRO_0000384057"
FT ACT_SITE 290
FT /evidence="ECO:0000250"
FT CARBOHYD 126
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 648
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 768 AA; 82985 MW; 952BE8E9F04C74F7 CRC64;
MILHKMAFLA VILFFLISSS SVCVHSRETF ACDTKDAATA TLRFCQLSVP IPERVRDLIG
RLTLAEKVSL LGNTAAAIPR LGIKGYEWWS EALHGVSNVG PGTKFGGVYP AATSFPQVIT
TVASFNASLW ESIGRVVSNE ARAMYNGGVG GLTYWSPNVN ILRDPRWGRG QETPGEDPVV
AGKYAASYVR GLQGNDRSRL KVAACCKHFT AYDLDNWNGV DRFHFNAKVS KQDIEDTFDV
PFRMCVKEGN VASIMCSYNQ VNGVPTCADP NLLKKTIRNQ WGLNGYIVSD CDSVGVLYDT
QHYTGTPEEA AADSIKAGLD LDCGPFLGAH TIDAVKKNLL RESDVDNALI NTLTVQMRLG
MFDGDIAAQP YGHLGPAHVC TPVHKGLALE AAQQGIVLLK NHGSSLPLSS QRHRTVAVIG
PNSDATVTMI GNYAGVACGY TSPVQGITGY ARTIHQKGCV DVHCMDDRLF DAAVEAARGA
DATVLVMGLD QSIEAEFKDR NSLLLPGKQQ ELVSRVAKAA KGPVILVLMS GGPIDISFAE
KDRKIPAIVW AGYPGQEGGT AIADILFGSA NPGGKLPMTW YPQDYLTNLP MTEMSMRPVH
SKRIPGRTYR FYDGPVVYPF GHGLSYTRFT HNIADAPKVI PIAVRGRNGT VSGKSIRVTH
ARCDRLSLGV HVEVTNVGSR DGTHTMLVFS APPGGEWAPK KQLVAFERVH VAVGEKKRVQ
VNIHVCKYLS VVDRAGNRRI PIGDHGIHIG DESHTVSLQA STLGVIKS
