BXL4_ARATH
ID BXL4_ARATH Reviewed; 784 AA.
AC Q9FLG1; Q56WR7;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Beta-D-xylosidase 4;
DE Short=AtBXL4;
DE EC=3.2.1.37;
DE Flags: Precursor;
GN Name=BXL4; OrderedLocusNames=At5g64570; ORFNames=MUB3.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 262-784.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=12609041; DOI=10.1046/j.1365-313x.2003.01654.x;
RA Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C.,
RA Joseleau J.-P., Jouanin L.;
RT "AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-
RT xylosidase gene, is involved in secondary cell wall metabolism and plant
RT development.";
RL Plant J. 33:677-690(2003).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE
RP SPECIFICITY.
RX PubMed=15181203; DOI=10.1104/pp.104.041269;
RA Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L.;
RT "Purification and characterization of enzymes exhibiting beta-D-xylosidase
RT activities in stem tissues of Arabidopsis.";
RL Plant Physiol. 135:867-878(2004).
CC -!- FUNCTION: Beta-D-xylosidase showing an optimal efficiency with the
CC natural substrate xylobiose. {ECO:0000269|PubMed:15181203}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-
CC xylose residues from the non-reducing termini.; EC=3.2.1.37;
CC Evidence={ECO:0000269|PubMed:15181203};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.7 mM for p-nitrophenyl-beta-D-xylopyranoside
CC {ECO:0000269|PubMed:15181203};
CC pH dependence:
CC Optimum pH is 4.7. {ECO:0000269|PubMed:15181203};
CC Temperature dependence:
CC Optimum temperature is 60 degrees Celsius.
CC {ECO:0000269|PubMed:15181203};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers, siliques and the apical part
CC of the stems. {ECO:0000269|PubMed:12609041,
CC ECO:0000269|PubMed:15181203}.
CC -!- MISCELLANEOUS: Might be processed at the C-terminus.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 3 family. {ECO:0000305}.
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DR EMBL; AB010076; BAB11424.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97922.1; -; Genomic_DNA.
DR EMBL; AK221967; BAD94481.1; -; mRNA.
DR RefSeq; NP_201262.1; NM_125853.3.
DR AlphaFoldDB; Q9FLG1; -.
DR SMR; Q9FLG1; -.
DR STRING; 3702.AT5G64570.1; -.
DR CAZy; GH3; Glycoside Hydrolase Family 3.
DR PaxDb; Q9FLG1; -.
DR PRIDE; Q9FLG1; -.
DR EnsemblPlants; AT5G64570.1; AT5G64570.1; AT5G64570.
DR GeneID; 836578; -.
DR Gramene; AT5G64570.1; AT5G64570.1; AT5G64570.
DR KEGG; ath:AT5G64570; -.
DR Araport; AT5G64570; -.
DR TAIR; locus:2174809; AT5G64570.
DR eggNOG; ENOG502QQ55; Eukaryota.
DR HOGENOM; CLU_004542_5_3_1; -.
DR InParanoid; Q9FLG1; -.
DR OMA; QCARDSN; -.
DR OrthoDB; 321444at2759; -.
DR PhylomeDB; Q9FLG1; -.
DR BioCyc; ARA:AT5G64570-MON; -.
DR BRENDA; 3.2.1.37; 399.
DR SABIO-RK; Q9FLG1; -.
DR PRO; PR:Q9FLG1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLG1; baseline and differential.
DR Genevisible; Q9FLG1; AT.
DR GO; GO:0048046; C:apoplast; HDA:TAIR.
DR GO; GO:0016020; C:membrane; HDA:TAIR.
DR GO; GO:0046556; F:alpha-L-arabinofuranosidase activity; IBA:GO_Central.
DR GO; GO:0009044; F:xylan 1,4-beta-xylosidase activity; IDA:TAIR.
DR GO; GO:0031222; P:arabinan catabolic process; IBA:GO_Central.
DR GO; GO:0009627; P:systemic acquired resistance; IEP:TAIR.
DR GO; GO:0045493; P:xylan catabolic process; IDA:TAIR.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.20.20.300; -; 1.
DR Gene3D; 3.40.50.1700; -; 1.
DR InterPro; IPR044993; BXL.
DR InterPro; IPR026891; Fn3-like.
DR InterPro; IPR002772; Glyco_hydro_3_C.
DR InterPro; IPR036881; Glyco_hydro_3_C_sf.
DR InterPro; IPR001764; Glyco_hydro_3_N.
DR InterPro; IPR036962; Glyco_hydro_3_N_sf.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR42721; PTHR42721; 1.
DR Pfam; PF14310; Fn3-like; 1.
DR Pfam; PF00933; Glyco_hydro_3; 1.
DR Pfam; PF01915; Glyco_hydro_3_C; 1.
DR PRINTS; PR00133; GLHYDRLASE3.
DR SMART; SM01217; Fn3_like; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF52279; SSF52279; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..38
FT /evidence="ECO:0000255"
FT CHAIN 39..784
FT /note="Beta-D-xylosidase 4"
FT /id="PRO_0000384059"
FT ACT_SITE 308
FT /evidence="ECO:0000250"
FT CARBOHYD 141
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 441
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 263
FT /note="C -> Y (in Ref. 3; BAD94481)"
FT /evidence="ECO:0000305"
FT CONFLICT 520
FT /note="H -> R (in Ref. 3; BAD94481)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 784 AA; 84308 MW; 52D404092378771A CRC64;
MGSSSPLTRR NRAPPSSVSS VYLIFLCFFL YFLNFSNAQS SPVFACDVAA NPSLAAYGFC
NTVLKIEYRV ADLVARLTLQ EKIGFLVSKA NGVTRLGIPT YEWWSEALHG VSYIGPGTHF
SSQVPGATSF PQVILTAASF NVSLFQAIGK VVSTEARAMY NVGLAGLTYW SPNVNIFRDP
RWGRGQETPG EDPLLASKYA SGYVKGLQET DGGDSNRLKV AACCKHYTAY DVDNWKGVER
YSFNAVVTQQ DMDDTYQPPF KSCVVDGNVA SVMCSYNQVN GKPTCADPDL LSGVIRGEWK
LNGYIVSDCD SVDVLYKNQH YTKTPAEAAA ISILAGLDLN CGSFLGQHTE EAVKSGLVNE
AAIDKAISNN FLTLMRLGFF DGNPKNQIYG GLGPTDVCTS ANQELAADAA RQGIVLLKNT
GCLPLSPKSI KTLAVIGPNA NVTKTMIGNY EGTPCKYTTP LQGLAGTVST TYLPGCSNVA
CAVADVAGAT KLAATADVSV LVIGADQSIE AESRDRVDLH LPGQQQELVI QVAKAAKGPV
LLVIMSGGGF DITFAKNDPK IAGILWVGYP GEAGGIAIAD IIFGRYNPSG KLPMTWYPQS
YVEKVPMTIM NMRPDKASGY PGRTYRFYTG ETVYAFGDGL SYTKFSHTLV KAPSLVSLGL
EENHVCRSSE CQSLDAIGPH CENAVSGGGS AFEVHIKVRN GGDREGIHTV FLFTTPPAIH
GSPRKHLVGF EKIRLGKREE AVVRFKVEIC KDLSVVDEIG KRKIGLGKHL LHVGDLKHSL
SIRI
