ID   TRMH_BORBU              Reviewed;         218 AA.
AC   O51081;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=tRNA (guanosine(18)-2'-O)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_02060};
DE            EC=2.1.1.34 {ECO:0000255|HAMAP-Rule:MF_02060};
DE   AltName: Full=tRNA [Gm18] methyltransferase {ECO:0000255|HAMAP-Rule:MF_02060};
GN   Name=trmH {ECO:0000255|HAMAP-Rule:MF_02060}; OrderedLocusNames=BB_0052;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
CC   -!- FUNCTION: Catalyzes the 2'-O methylation of guanosine at position 18 in
CC       tRNA. {ECO:0000255|HAMAP-Rule:MF_02060}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(18) in tRNA + S-adenosyl-L-methionine = 2'-O-
CC         methylguanosine(18) in tRNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:20077, Rhea:RHEA-COMP:10190, Rhea:RHEA-COMP:10192,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74445; EC=2.1.1.34;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02060};
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. RNA methyltransferase TrmH family. {ECO:0000255|HAMAP-
CC       Rule:MF_02060}.
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DR   EMBL; AE000783; AAC66431.1; -; Genomic_DNA.
DR   PIR; D70106; D70106.
DR   RefSeq; NP_212186.1; NC_001318.1.
DR   RefSeq; WP_002658321.1; NC_001318.1.
DR   AlphaFoldDB; O51081; -.
DR   SMR; O51081; -.
DR   STRING; 224326.BB_0052; -.
DR   EnsemblBacteria; AAC66431; AAC66431; BB_0052.
DR   GeneID; 56568165; -.
DR   KEGG; bbu:BB_0052; -.
DR   PATRIC; fig|224326.49.peg.450; -.
DR   HOGENOM; CLU_021322_4_1_12; -.
DR   OMA; KIPMVGF; -.
DR   Proteomes; UP000001807; Chromosome.
DR   GO; GO:0009020; F:tRNA (guanosine-2'-O-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002938; P:tRNA guanine ribose methylation; IEA:UniProtKB-UniRule.
DR   CDD; cd18092; SpoU-like_TrmH; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   HAMAP; MF_02060; tRNA_methyltr_TrmH; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR001537; SpoU_MeTrfase.
DR   InterPro; IPR033671; TrmH.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR43453; PTHR43453; 1.
DR   Pfam; PF00588; SpoU_methylase; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; RNA-binding;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing; tRNA-binding.
FT   CHAIN           1..218
FT                   /note="tRNA (guanosine(18)-2'-O)-methyltransferase"
FT                   /id="PRO_0000159822"
FT   BINDING         111
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02060"
FT   BINDING         154
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02060"
SQ   SEQUENCE   218 AA;  24773 MW;  14BFF3D84151EB6E CRC64;
     MDDDVLKRIG VLSKFITDEK KARIEKVLNL RTNYLTFVLE DIFQSQNASA TIRTGEILGL
     SDVHVIEKKN KHTLNPDVTL GSSQWINLNK YKNAKFAIDR LKSDGYSIVA TSLNPKSINL
     ENLTINNKMA IFFGTELTGL SAEVLGAADL YVKIPMYGFT QSYNISVAVA IVMYSLLMRL
     RKSSIDYLLN EAEKSNLRLK YYRQVVKNYQ FIENLINS