BYST_ARATH
ID BYST_ARATH Reviewed; 444 AA.
AC Q8RWS4; Q9C6V6;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Bystin {ECO:0000305};
DE AltName: Full=Essential nuclear protein 1 {ECO:0000303|PubMed:23382868};
DE Short=AtEnp1 {ECO:0000303|PubMed:23382868};
GN Name=ENP1 {ECO:0000303|PubMed:23382868};
GN OrderedLocusNames=At1g31660 {ECO:0000312|Araport:AT1G31660};
GN ORFNames=F27M3.14 {ECO:0000312|EMBL:AAG60158.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND SUBUNIT.
RC STRAIN=cv. Columbia;
RX PubMed=23382868; DOI=10.1371/journal.pone.0054084;
RA Missbach S., Weis B.L., Martin R., Simm S., Bohnsack M.T., Schleiff E.;
RT "40S ribosome biogenesis co-factors are essential for gametophyte and
RT embryo development.";
RL PLoS ONE 8:E54084-E54084(2013).
CC -!- FUNCTION: Essential protein required during embryogenesis and pollen
CC development (PubMed:23382868). Required for processing of 20S pre-rRNA
CC precursor and biogenesis of 40S ribosomal subunits (PubMed:23382868).
CC {ECO:0000269|PubMed:23382868}.
CC -!- SUBUNIT: Component of the 40S pre-ribosome.
CC {ECO:0000269|PubMed:23382868}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:23382868}.
CC Nucleus, nucleoplasm {ECO:0000269|PubMed:23382868}.
CC -!- TISSUE SPECIFICITY: Highly expressed in flowers and at lower levels in
CC roots, hypocotyls, stems, leaves, siliques and seeds.
CC {ECO:0000269|PubMed:23382868}.
CC -!- DISRUPTION PHENOTYPE: Delayed pollen development with reduced nuclei
CC content leading to reduced pollen germination capacity, and pale seeds
CC with arrested embryo development at the globular stage in homozygous
CC plants (PubMed:23382868). In heterozygous plants, slight accumulation
CC of the 23S-like precursor P-A3 (PubMed:23382868). Reduced siliques size
CC with small non-developed and early aborted seeds (PubMed:23382868).
CC {ECO:0000269|PubMed:23382868}.
CC -!- SIMILARITY: Belongs to the bystin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG60158.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC074360; AAG60158.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31381.1; -; Genomic_DNA.
DR EMBL; AY091144; AAM14093.1; -; mRNA.
DR EMBL; BT003812; AAO41865.1; -; mRNA.
DR RefSeq; NP_174447.2; NM_102901.4.
DR AlphaFoldDB; Q8RWS4; -.
DR SMR; Q8RWS4; -.
DR STRING; 3702.AT1G31660.1; -.
DR PaxDb; Q8RWS4; -.
DR PRIDE; Q8RWS4; -.
DR ProteomicsDB; 179361; -.
DR EnsemblPlants; AT1G31660.1; AT1G31660.1; AT1G31660.
DR GeneID; 840053; -.
DR Gramene; AT1G31660.1; AT1G31660.1; AT1G31660.
DR KEGG; ath:AT1G31660; -.
DR Araport; AT1G31660; -.
DR TAIR; locus:2028686; AT1G31660.
DR eggNOG; KOG3871; Eukaryota.
DR HOGENOM; CLU_029727_0_1_1; -.
DR InParanoid; Q8RWS4; -.
DR OMA; AGGQEKH; -.
DR OrthoDB; 1193442at2759; -.
DR PhylomeDB; Q8RWS4; -.
DR PRO; PR:Q8RWS4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RWS4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IBA:GO_Central.
DR GO; GO:0030515; F:snoRNA binding; IBA:GO_Central.
DR GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0006364; P:rRNA processing; IMP:TAIR.
DR InterPro; IPR007955; Bystin.
DR PANTHER; PTHR12821; PTHR12821; 1.
DR Pfam; PF05291; Bystin; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ribosome biogenesis; rRNA processing.
FT CHAIN 1..444
FT /note="Bystin"
FT /id="PRO_0000448722"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 53..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 444 AA; 51473 MW; 18646DF14AF97F57 CRC64;
MAKKRDRIVN TQPFISDDAS VASSRKRSKV PKTHQKQEKL IEAGMSEKIM KQALAQQKEV
ADEENAERNP SSAAFAVAGA ATAGEEQKIL EEEEDDIDDF DGTFENQSQF DKQEEINEDD
EKLFESFLNK NAPPQRTLTD IIIKKLKDKD ADLAEEERPD PKMDPAITKL YKGVGKFMSE
YTVGKLPKAF KLVTSMEHWE DVLYLTEPEK WSPNALYQAT RIFASNLKDR QVQRFYNYVL
LPRVREDIRK HKKLHFALYQ ALKKSLYKPS AFNQGILFPL CKSGTCNLRE AVIIGSILEK
CSIPMLHSCV ALNRLAEMDY CGTTSYFIKV LLEKKYCMPY RVLDALVAHF MRFVDDIRVM
PVIWHQSLLT FVQRYKYEIL KEDKEHLQTL LQRQKHHLVT PEILRELKDS RNRGEKEDPM
VDNFAPVPAK EDRFDIPEVP MEED
