TRMN6_ECOLI
ID TRMN6_ECOLI Reviewed; 245 AA.
AC P31825; P76591; Q2MAF5;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=tRNA1(Val) (adenine(37)-N6)-methyltransferase;
DE EC=2.1.1.223;
DE AltName: Full=tRNA m6A37 methyltransferase;
GN Name=yfiC; OrderedLocusNames=b2575, JW2559;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Nashimoto H.;
RT "Non-ribosomal proteins affecting the assembly of ribosomes in Escherichia
RT coli.";
RL (In) Nierhaus K.H. (eds.);
RL The translational apparatus, pp.185-195, Plenum Press, New York (1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Nashimoto H., Saito N.;
RL Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SIMILARITY TO METHYLTRANSFERASES.
RA Baum B., Koonin E.V.;
RL Unpublished observations (MAY-1993).
RN [6]
RP FUNCTION AS A METHYLTRANSFERASE, CATALYTIC ACTIVITY, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=BW25141;
RX PubMed=19383770; DOI=10.1261/rna.1494409;
RA Golovina A.Y., Sergiev P.V., Golovin A.V., Serebryakova M.V., Demina I.,
RA Govorun V.M., Dontsova O.A.;
RT "The yfiC gene of E. coli encodes an adenine-N6 methyltransferase that
RT specifically modifies A37 of tRNA1Val(cmo5UAC).";
RL RNA 15:1134-1141(2009).
CC -!- FUNCTION: Specifically methylates the adenine in position 37 of
CC tRNA(1)(Val) (anticodon cmo5UAC). {ECO:0000269|PubMed:19383770}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenosine(37) in tRNA1(Val) + S-adenosyl-L-methionine = H(+) +
CC N(6)-methyladenosine(37) in tRNA1(Val) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43160, Rhea:RHEA-COMP:10369, Rhea:RHEA-COMP:10370,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.223;
CC Evidence={ECO:0000269|PubMed:19383770};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene show hypersensitivity to
CC hyperosmotic and, to a lesser extent, oxidative stress.
CC {ECO:0000269|PubMed:19383770}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. tRNA
CC (adenine-N(6)-)-methyltransferase family. {ECO:0000305}.
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DR EMBL; D13169; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; D64044; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00096; AAC75628.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE76751.1; -; Genomic_DNA.
DR PIR; F65035; F65035.
DR RefSeq; NP_417070.2; NC_000913.3.
DR RefSeq; WP_001295363.1; NZ_SSZK01000005.1.
DR AlphaFoldDB; P31825; -.
DR SMR; P31825; -.
DR BioGRID; 4260913; 33.
DR IntAct; P31825; 4.
DR STRING; 511145.b2575; -.
DR jPOST; P31825; -.
DR PaxDb; P31825; -.
DR PRIDE; P31825; -.
DR EnsemblBacteria; AAC75628; AAC75628; b2575.
DR EnsemblBacteria; BAE76751; BAE76751; BAE76751.
DR GeneID; 947047; -.
DR KEGG; ecj:JW2559; -.
DR KEGG; eco:b2575; -.
DR PATRIC; fig|511145.12.peg.2677; -.
DR EchoBASE; EB1500; -.
DR eggNOG; COG4123; Bacteria.
DR HOGENOM; CLU_061983_0_0_6; -.
DR InParanoid; P31825; -.
DR OMA; NQYTEAF; -.
DR PhylomeDB; P31825; -.
DR BioCyc; EcoCyc:EG11538-MON; -.
DR BioCyc; MetaCyc:EG11538-MON; -.
DR BRENDA; 2.1.1.223; 2026.
DR PRO; PR:P31825; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0016430; F:tRNA (adenine-N6-)-methyltransferase activity; IDA:EcoCyc.
DR GO; GO:0030488; P:tRNA methylation; IMP:EcoCyc.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_01872; tRNA_methyltr_YfiC; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR007848; Small_mtfrase_dom.
DR InterPro; IPR022882; tRNA_adenine-N6_MeTrfase.
DR Pfam; PF05175; MTS; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase; tRNA processing.
FT CHAIN 1..245
FT /note="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
FT /id="PRO_0000169266"
FT CONFLICT 83
FT /note="A -> R (in Ref. 1; D13169 and 2; D64044)"
FT /evidence="ECO:0000305"
FT CONFLICT 211..245
FT /note="PQAGECFSDRLVIRGPDQNYSEAYTALTQAFYLFM -> RRRENALAIA
FT (in Ref. 1; D13169 and 2; D64044)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 245 AA; 27270 MW; CEB4F1E3A849B7E3 CRC64;
MSQSTSVLRR NGFTFKQFFV AHDRCAMKVG TDGILLGAWA PVAGVKRCLD IGAGSGLLAL
MLAQRTDDSV MIDAVELESE AAAQAQENIN QSPWAERINV HTADIQQWIT QQTVRFDLII
SNPPYYQQGV ECSTPQREQA RYTTTLDHPS LLTCAAECIT EEGFFCVVLP EQIGNGFTEL
ALSMGWHLRL RTDVAENEAR LPHRVLLAFS PQAGECFSDR LVIRGPDQNY SEAYTALTQA
FYLFM
