TRMYL_VIBCH
ID TRMYL_VIBCH Reviewed; 201 AA.
AC Q9KKP3;
DT 25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Putative pseudouridine methyltransferase {ECO:0000255|HAMAP-Rule:MF_00587};
DE EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_00587};
GN OrderedLocusNames=VC_A1059;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
RG Midwest center for structural genomics (MCSG);
RT "Crystal structure of unknown function protein VCA1059.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00587}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. TrmY family.
CC {ECO:0000255|HAMAP-Rule:MF_00587}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF96953.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE003853; AAF96953.1; ALT_INIT; Genomic_DNA.
DR PIR; A82384; A82384.
DR RefSeq; NP_233441.1; NC_002506.1.
DR RefSeq; WP_001256106.1; NZ_LT906615.1.
DR PDB; 2QWV; X-ray; 2.60 A; A/B=1-201.
DR PDBsum; 2QWV; -.
DR AlphaFoldDB; Q9KKP3; -.
DR SMR; Q9KKP3; -.
DR STRING; 243277.VC_A1059; -.
DR PRIDE; Q9KKP3; -.
DR DNASU; 2612057; -.
DR EnsemblBacteria; AAF96953; AAF96953; VC_A1059.
DR GeneID; 57742410; -.
DR KEGG; vch:VC_A1059; -.
DR PATRIC; fig|243277.26.peg.3665; -.
DR eggNOG; COG1901; Bacteria.
DR HOGENOM; CLU_107018_0_0_6; -.
DR OMA; HADHCII; -.
DR EvolutionaryTrace; Q9KKP3; -.
DR Proteomes; UP000000584; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IBA:GO_Central.
DR GO; GO:0008175; F:tRNA methyltransferase activity; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR CDD; cd18087; TrmY-like; 1.
DR Gene3D; 3.40.1280.10; -; 1.
DR HAMAP; MF_00587; tRNA_methyltr_TrmY; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR007158; TrmY.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR PANTHER; PTHR40703; PTHR40703; 1.
DR Pfam; PF04013; Methyltrn_RNA_2; 1.
DR SUPFAM; SSF75217; SSF75217; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..201
FT /note="Putative pseudouridine methyltransferase"
FT /id="PRO_0000157956"
FT BINDING 132
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00587"
FT BINDING 186
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00587"
FT STRAND 2..14
FT /evidence="ECO:0007829|PDB:2QWV"
FT HELIX 15..20
FT /evidence="ECO:0007829|PDB:2QWV"
FT TURN 21..23
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 24..26
FT /evidence="ECO:0007829|PDB:2QWV"
FT HELIX 29..39
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 47..57
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 63..68
FT /evidence="ECO:0007829|PDB:2QWV"
FT TURN 69..71
FT /evidence="ECO:0007829|PDB:2QWV"
FT HELIX 80..93
FT /evidence="ECO:0007829|PDB:2QWV"
FT TURN 94..96
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 102..106
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 109..112
FT /evidence="ECO:0007829|PDB:2QWV"
FT HELIX 116..124
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 127..132
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 136..138
FT /evidence="ECO:0007829|PDB:2QWV"
FT TURN 139..141
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 146..152
FT /evidence="ECO:0007829|PDB:2QWV"
FT TURN 166..170
FT /evidence="ECO:0007829|PDB:2QWV"
FT STRAND 172..175
FT /evidence="ECO:0007829|PDB:2QWV"
FT HELIX 183..199
FT /evidence="ECO:0007829|PDB:2QWV"
SQ SEQUENCE 201 AA; 22275 MW; 83AAB4EF25C7A632 CRC64;
MRSFILRARS APTDSQRLLD EIGGKCHTEI LAHCMMNSLF TAQSHREDVV IHLVLESTRD
YSRTITVEAN EISDVGGFHE AALIALLVKA LDASVGMGKE QTRVVQPGLT VRTISFEALL
GELAEHHSLY MMDKKGDSIR DIKIGPNPCF ILTDHIPMPK KSGNSMKRLG VEKISLGPKM
LFASQCVTLI HNEIDHQEAG W
