TRN2_ARATH
ID TRN2_ARATH Reviewed; 269 AA.
AC Q9FIQ5; Q8VYM8;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein TORNADO 2;
DE AltName: Full=Protein EKEKO;
DE AltName: Full=TETRASPANIN-1;
GN Name=TRN2; Synonyms=TET1; OrderedLocusNames=At5g46700; ORFNames=MZA15.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=10887093; DOI=10.1242/dev.127.15.3385;
RA Cnops G., Wang X., Linstead P., Van Montagu M., Van Lijsebettens M.,
RA Dolan L.;
RT "Tornado1 and tornado2 are required for the specification of radial and
RT circumferential pattern in the Arabidopsis root.";
RL Development 127:3385-3394(2000).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=14559222; DOI=10.1016/j.bbrc.2003.09.122;
RA Olmos E., Reiss B., Dekker K.;
RT "The ekeko mutant demonstrates a role for tetraspanin-like protein in plant
RT development.";
RL Biochem. Biophys. Res. Commun. 310:1054-1061(2003).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF PRO-164 AND GLY-177, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16531491; DOI=10.1105/tpc.105.040568;
RA Cnops G., Neyt P., Raes J., Petrarulo M., Nelissen H., Malenica N.,
RA Luschnig C., Tietz O., Ditengou F., Palme K., Azmi A., Prinsen E.,
RA Van Lijsebettens M.;
RT "The TORNADO1 and TORNADO2 genes function in several patterning processes
RT during early leaf development in Arabidopsis thaliana.";
RL Plant Cell 18:852-866(2006).
RN [9]
RP FUNCTION, MUTAGENESIS OF GLY-177, AND DEVELOPMENTAL STAGE.
RX PubMed=17351828; DOI=10.1007/s11103-006-9105-z;
RA Chiu W.-H., Chandler J., Cnops G., Van Lijsebettens M., Werr W.;
RT "Mutations in the TORNADO2 gene affect cellular decisions in the peripheral
RT zone of the shoot apical meristem of Arabidopsis thaliana.";
RL Plant Mol. Biol. 63:731-744(2007).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=21658947; DOI=10.1016/j.cub.2011.05.015;
RA Lieber D., Lora J., Schrempp S., Lenhard M., Laux T.;
RT "Arabidopsis WIH1 and WIH2 genes act in the transition from somatic to
RT reproductive cell fate.";
RL Curr. Biol. 21:1009-1017(2011).
CC -!- FUNCTION: Involved in the basipetal transport of auxin (IAA) that
CC modulates growth and organs organization, as well as cell
CC differentiation. Regulates shoot apical meristem (SAM) organization in
CC the peripheral zone. Required for initial meristematic divisions in the
CC epidermal/lateral root cap leading to the formation of epidermal cells
CC and a clone of lateral root cap cells, as well as for the maintenance
CC of the radial pattern of cell specification in the root, thus
CC regulating the distinction between the lateral root cap and epidermis.
CC Together with WIH peptides, promotes megasporogenesis.
CC {ECO:0000269|PubMed:10887093, ECO:0000269|PubMed:14559222,
CC ECO:0000269|PubMed:16531491, ECO:0000269|PubMed:17351828,
CC ECO:0000269|PubMed:21658947}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, roots, leaves, stems, apex,
CC siliques and flowers. Present in ovules, prominently in nucellus and
CC integuments. {ECO:0000269|PubMed:14559222, ECO:0000269|PubMed:16531491,
CC ECO:0000269|PubMed:21658947}.
CC -!- DEVELOPMENTAL STAGE: Strongly expressed in all types of meristems,
CC including the shoot apical meristem (SAM) and lateral organ primordia.
CC Also detected in the lamina of the cotyledons, especially in the
CC mesophyll and vascular bundles. In leaf primorida preferentially
CC present in vascular strands and at the distal tip of the leaflet. In
CC the floral meristem, first detected in flanking sepal primordia, and
CC later expressed in stamens and carpels in flowers.
CC {ECO:0000269|PubMed:16531491, ECO:0000269|PubMed:17351828}.
CC -!- DISRUPTION PHENOTYPE: Severe dwarfism combined with twisted and
CC malformed organs, and sterility. Loss of initial meristematic divisions
CC in the epidermal/lateral root cap. Defection in basipetal transport of
CC auxin (IAA) leading to several development aberrations.
CC {ECO:0000269|PubMed:10887093, ECO:0000269|PubMed:14559222,
CC ECO:0000269|PubMed:16531491, ECO:0000269|PubMed:21658947}.
CC -!- MISCELLANEOUS: 'Ekeko' is derived from Inca language (quechua) for
CC small and hunchbacked fortune god. {ECO:0000305|PubMed:14559222}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR EMBL; AB016882; BAB08914.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95415.1; -; Genomic_DNA.
DR EMBL; AY070423; AAL49918.1; -; mRNA.
DR EMBL; BT020321; AAV85676.1; -; mRNA.
DR EMBL; AK226278; BAE98437.1; -; mRNA.
DR RefSeq; NP_199482.1; NM_124040.5.
DR AlphaFoldDB; Q9FIQ5; -.
DR STRING; 3702.AT5G46700.1; -.
DR PaxDb; Q9FIQ5; -.
DR PRIDE; Q9FIQ5; -.
DR ProteomicsDB; 234589; -.
DR EnsemblPlants; AT5G46700.1; AT5G46700.1; AT5G46700.
DR GeneID; 834713; -.
DR Gramene; AT5G46700.1; AT5G46700.1; AT5G46700.
DR KEGG; ath:AT5G46700; -.
DR Araport; AT5G46700; -.
DR TAIR; locus:2178570; AT5G46700.
DR eggNOG; ENOG502QQRT; Eukaryota.
DR HOGENOM; CLU_066970_0_0_1; -.
DR InParanoid; Q9FIQ5; -.
DR OMA; ADMDCLN; -.
DR OrthoDB; 1166716at2759; -.
DR PRO; PR:Q9FIQ5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FIQ5; baseline and differential.
DR Genevisible; Q9FIQ5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010305; P:leaf vascular tissue pattern formation; IMP:TAIR.
DR GO; GO:0009554; P:megasporogenesis; IMP:UniProtKB.
DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR.
DR GO; GO:0009956; P:radial pattern formation; IMP:TAIR.
DR GO; GO:0009934; P:regulation of meristem structural organization; IMP:UniProtKB.
DR GO; GO:0010015; P:root morphogenesis; IMP:TAIR.
DR InterPro; IPR044991; TET_plant.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR PANTHER; PTHR32191; PTHR32191; 1.
DR Pfam; PF00335; Tetraspanin; 1.
PE 1: Evidence at protein level;
KW Auxin signaling pathway; Developmental protein; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..269
FT /note="Protein TORNADO 2"
FT /id="PRO_0000421041"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..44
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..71
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..231
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..269
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 200
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 164
FT /note="P->L: In trn2-3; twisted organs and impaired auxin
FT flux."
FT /evidence="ECO:0000269|PubMed:16531491"
FT MUTAGEN 177
FT /note="G->E: In trn2-2; twisted organs and impaired auxin
FT flux, with an enlarged peripheral zone in shoot apical
FT meristem (SAM)."
FT /evidence="ECO:0000269|PubMed:16531491,
FT ECO:0000269|PubMed:17351828"
FT CONFLICT 187
FT /note="I -> V (in Ref. 3; AAL49918)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 269 AA; 30287 MW; 7D43A8223F3D0962 CRC64;
MPLSNNVIGC INFITVLLSI PVIGAGIWLA IGTVNSCVKL LQWPVIILGV LILLVGLAGF
IGGFWRITWL LVVYLIAMLI LIVLLGCLVG FIYMVTIRGS GHPEPSRAYL EYSLQDFSGW
LRRRVQRSYK WERIRTCLST TTICPELNQR YTLAQDFFNA HLDPIQSGCC KPPTKCGFTF
VNPTYWISPI DMSADMDCLN WSNDQNTLCY TCDSCKAGLL ANIKVDWLKA DIFLLLALIG
LIIVYIIGCC AFRNAETEDI FRKYKQGYT
