ID   TRN2_HYONI              Reviewed;         260 AA.
AC   P50164;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Tropinone reductase 2;
DE            EC=1.1.1.236;
DE   AltName: Full=Tropinone reductase II;
DE            Short=TR-II;
GN   Name=TR2;
OS   Hyoscyamus niger (Black henbane).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Hyoscyameae;
OC   Hyoscyamus.
OX   NCBI_TaxID=4079;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Cultured root;
RX   PubMed=8290643; DOI=10.1104/pp.103.4.1465;
RA   Nakajima K., Hashimoto T., Yamada Y.;
RT   "cDNA encoding tropinone reductase-II from Hyoscyamus niger.";
RL   Plant Physiol. 103:1465-1466(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11855731; DOI=10.1023/a:1013315110746;
RA   Keiner R., Kaiser H., Nakajima K., Hashimoto T., Drager B.;
RT   "Molecular cloning, expression and characterization of tropinone reductase
RT   II, an enzyme of the SDR family in Solanum tuberosum (L.).";
RL   Plant Mol. Biol. 48:299-308(2002).
CC   -!- FUNCTION: Catalyzes the stereospecific reduction of tropinone to
CC       pseudotropine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NADP(+) + pseudotropine = H(+) + NADPH + tropinone;
CC         Xref=Rhea:RHEA:24244, ChEBI:CHEBI:15378, ChEBI:CHEBI:57493,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:57851, ChEBI:CHEBI:58349;
CC         EC=1.1.1.236;
CC   -!- PATHWAY: Alkaloid biosynthesis; tropane alkaloid biosynthesis.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; L20485; AAB09776.1; -; mRNA.
DR   EMBL; AB026545; BAA85845.1; -; Genomic_DNA.
DR   AlphaFoldDB; P50164; -.
DR   SMR; P50164; -.
DR   UniPathway; UPA00330; -.
DR   GO; GO:0050358; F:tropinone reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009710; P:tropane alkaloid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   InterPro; IPR045000; TR.
DR   PANTHER; PTHR42898; PTHR42898; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   2: Evidence at transcript level;
KW   NADP; Oxidoreductase.
FT   CHAIN           1..260
FT                   /note="Tropinone reductase 2"
FT                   /id="PRO_0000054787"
FT   ACT_SITE        159
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         13..37
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         146
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   260 AA;  28437 MW;  6CA7AF85CAA128FC CRC64;
     MAGRWNLEGC TALVTGGSRG IGYGIVEELA NLGASVYTCS RNQKELDECL TQWRSKGFNV
     EASVCDLSSR SEREEFMKTV SNHFHGKLNI LVNNAGIVIY KEAKDYTMED YSHIMSINFE
     AAYHLSVLAH PFLKASERGN VVFISSISGA SALPYEAVYG ATKGAMDQLT RCLAFEWAKD
     NIRVNGVGPG VIATSMVEMT IQDPEQKENL DKLIDRCALR RMGEPKELAA VVAFLCFPAA
     SYVTGQIIYV DGGFMANGGF