ID   TROPF_TALSN             Reviewed;         306 AA.
AC   B8M9K3;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Esterase tropF {ECO:0000303|PubMed:22508998};
DE            EC=3.1.2.- {ECO:0000269|PubMed:24863423};
DE   AltName: Full=Tropolone synthesis protein F {ECO:0000303|PubMed:24863423};
GN   Name=tropF {ECO:0000303|PubMed:24863423};
GN   Synonyms=tsR3 {ECO:0000303|PubMed:22508998}; ORFNames=TSTA_117780;
OS   Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL
OS   1006) (Penicillium stipitatum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces;
OC   Talaromyces sect. Talaromyces.
OX   NCBI_TaxID=441959;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006;
RX   PubMed=22508998; DOI=10.1073/pnas.1201469109;
RA   Davison J., al Fahad A., Cai M., Song Z., Yehia S.Y., Lazarus C.M.,
RA   Bailey A.M., Simpson T.J., Cox R.J.;
RT   "Genetic, molecular, and biochemical basis of fungal tropolone
RT   biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:7642-7647(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006;
RX   PubMed=24863423; DOI=10.1002/anie.201403450;
RA   al Fahad A., Abood A., Simpson T.J., Cox R.J.;
RT   "The biosynthesis and catabolism of the maleic anhydride moiety of
RT   stipitatonic acid.";
RL   Angew. Chem. Int. Ed. 53:7519-7523(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006;
RX   PubMed=25676766; DOI=10.1128/genomea.01559-14;
RA   Nierman W.C., Fedorova-Abrams N.D., Andrianopoulos A.;
RT   "Genome sequence of the AIDS-associated pathogen Penicillium marneffei
RT   (ATCC18224) and its near taxonomic relative Talaromyces stipitatus
RT   (ATCC10500).";
RL   Genome Announc. 3:E0155914-E0155914(2015).
CC   -!- FUNCTION: Esterase; part of the gene cluster that mediates the
CC       biosynthesis of the tropolone class of fungal maleic anhydrides
CC       (PubMed:22508998, PubMed:24863423). The pathway begins with the
CC       synthesis of 3-methylorcinaldehyde by the non-reducing polyketide
CC       synthase (PKS) tropA (PubMed:22508998). 3-methylorcinaldehyde is the
CC       substrate for the FAD-dependent monooxygenase tropB to yield a
CC       dearomatized hydroxycyclohexadione (PubMed:22508998, PubMed:24863423).
CC       The 2-oxoglutarate-dependent dioxygenase tropC then performs the
CC       oxidative ring expansion to provide the first tropolone metabolite
CC       stipitaldehyde (PubMed:22508998, PubMed:24863423). Trop D converts
CC       stipitaldehyde into stipitacetal which is in turn converted to
CC       stipitalide by the short-chain dehydrogenase/reductase tropE
CC       (PubMed:24863423). The next steps involve tropF, tropG, tropH, tropI
CC       and tropJ to form successive tropolone maleic anhydrides including
CC       stipitaldehydic, stipitatonic and stipitatic acids (PubMed:24863423).
CC       {ECO:0000269|PubMed:22508998, ECO:0000269|PubMed:24863423}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:24863423}.
CC   -!- SIMILARITY: Belongs to the LovG family. {ECO:0000305}.
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DR   EMBL; BK008910; DAA64707.1; -; Genomic_DNA.
DR   EMBL; EQ962655; EED18005.1; -; Genomic_DNA.
DR   RefSeq; XP_002481997.1; XM_002481952.1.
DR   AlphaFoldDB; B8M9K3; -.
DR   SMR; B8M9K3; -.
DR   EnsemblFungi; EED18005; EED18005; TSTA_117780.
DR   GeneID; 8105836; -.
DR   VEuPathDB; FungiDB:TSTA_117780; -.
DR   eggNOG; KOG2551; Eukaryota.
DR   HOGENOM; CLU_051938_0_0_1; -.
DR   InParanoid; B8M9K3; -.
DR   OMA; VWLRDHR; -.
DR   OrthoDB; 1190789at2759; -.
DR   PhylomeDB; B8M9K3; -.
DR   Proteomes; UP000001745; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR005645; FSH_dom.
DR   Pfam; PF03959; FSH1; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..306
FT                   /note="Esterase tropF"
FT                   /id="PRO_0000437133"
FT   ACT_SITE        147
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
FT   ACT_SITE        248
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
FT   ACT_SITE        276
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
SQ   SEQUENCE   306 AA;  34110 MW;  BA0DB02C3BA691FC CRC64;
     MLHGMSPECA LSKLLSSLEF HTLYRYTMTQ KHNFPSNNKD TLHLPRILCL HGGGTNARIF
     RAQCRALEKA LRTSFRLCYA EALFPSQPGP DVTAVYRDFG PFRAWIDSPD ANPVTMTEAL
     QISICKAIME DDQRGATGPV VGLLGFSQGA KVCASLILEQ QLLGRTFGDH SHLPFRLPQW
     RFAVLLAGRG PLVTLSHLSV GADIMQKMAH LCENDDSSGL DSQENLGNVI LTRELIQVPT
     IHVHGRKDPA LDLHRKLYYD DFDPRYSKVM EWDGAHRVPL KSKDVATLVK EINVLWASVS
     HSNVQG