ID   TROPI_TALSN             Reviewed;         254 AA.
AC   B8M9K0;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Hydrolase tropI {ECO:0000303|PubMed:22508998};
DE            EC=3.1.1.- {ECO:0000269|PubMed:24863423};
DE   AltName: Full=Tropolone synthesis protein I {ECO:0000303|PubMed:24863423};
GN   Name=tropI {ECO:0000303|PubMed:24863423};
GN   Synonyms=tsR1 {ECO:0000303|PubMed:22508998}; ORFNames=TSTA_117760;
OS   Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL
OS   1006) (Penicillium stipitatum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces;
OC   Talaromyces sect. Talaromyces.
OX   NCBI_TaxID=441959;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006;
RX   PubMed=22508998; DOI=10.1073/pnas.1201469109;
RA   Davison J., al Fahad A., Cai M., Song Z., Yehia S.Y., Lazarus C.M.,
RA   Bailey A.M., Simpson T.J., Cox R.J.;
RT   "Genetic, molecular, and biochemical basis of fungal tropolone
RT   biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:7642-7647(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006;
RX   PubMed=24863423; DOI=10.1002/anie.201403450;
RA   al Fahad A., Abood A., Simpson T.J., Cox R.J.;
RT   "The biosynthesis and catabolism of the maleic anhydride moiety of
RT   stipitatonic acid.";
RL   Angew. Chem. Int. Ed. 53:7519-7523(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006;
RX   PubMed=25676766; DOI=10.1128/genomea.01559-14;
RA   Nierman W.C., Fedorova-Abrams N.D., Andrianopoulos A.;
RT   "Genome sequence of the AIDS-associated pathogen Penicillium marneffei
RT   (ATCC18224) and its near taxonomic relative Talaromyces stipitatus
RT   (ATCC10500).";
RL   Genome Announc. 3:E0155914-E0155914(2015).
CC   -!- FUNCTION: Hydrolase; part of the gene cluster that mediates the
CC       biosynthesis of the tropolone class of fungal maleic anhydrides
CC       (PubMed:22508998, PubMed:24863423). The pathway begins with the
CC       synthesis of 3-methylorcinaldehyde by the non-reducing polyketide
CC       synthase (PKS) tropA (PubMed:22508998). 3-methylorcinaldehyde is the
CC       substrate for the FAD-dependent monooxygenase tropB to yield a
CC       dearomatized hydroxycyclohexadione (PubMed:22508998, PubMed:24863423).
CC       The 2-oxoglutarate-dependent dioxygenase tropC then performs the
CC       oxidative ring expansion to provide the first tropolone metabolite
CC       stipitaldehyde (PubMed:22508998, PubMed:24863423). Trop D converts
CC       stipitaldehyde into stipitacetal which is in turn converted to
CC       stipitalide by the short-chain dehydrogenase/reductase tropE
CC       (PubMed:24863423). The next steps involve tropF, tropG, tropH, tropI
CC       and tropJ to form successive tropolone maleic anhydrides including
CC       stipitaldehydic, stipitatonic and stipitatic acids (PubMed:24863423).
CC       {ECO:0000269|PubMed:22508998, ECO:0000269|PubMed:24863423}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:24863423}.
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BK008910; DAA64704.1; -; Genomic_DNA.
DR   EMBL; EQ962655; EED18002.1; -; Genomic_DNA.
DR   RefSeq; XP_002481994.1; XM_002481949.1.
DR   AlphaFoldDB; B8M9K0; -.
DR   SMR; B8M9K0; -.
DR   EnsemblFungi; EED18002; EED18002; TSTA_117760.
DR   GeneID; 8105834; -.
DR   VEuPathDB; FungiDB:TSTA_117760; -.
DR   eggNOG; KOG3043; Eukaryota.
DR   InParanoid; B8M9K0; -.
DR   OMA; CYGGRYV; -.
DR   OrthoDB; 1275897at2759; -.
DR   PhylomeDB; B8M9K0; -.
DR   Proteomes; UP000001745; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..254
FT                   /note="Hydrolase tropI"
FT                   /id="PRO_0000437135"
FT   ACT_SITE        141
FT                   /evidence="ECO:0000250|UniProtKB:P0A114"
FT   ACT_SITE        187
FT                   /evidence="ECO:0000250|UniProtKB:P0A114"
FT   ACT_SITE        219
FT                   /evidence="ECO:0000250|UniProtKB:P0A114"
SQ   SEQUENCE   254 AA;  28201 MW;  67753F51FEA48805 CRC64;
     MASLPSQSCC YQGFRHSGAP KGTLSMVKDI EVYTSYPPDK STEYGVLILT DIVGHRFSNA
     QIIADQFAEN GYFVMMPDLF LGDAVPLNKP GEFDMGKWRS GAYHPQGKNH LPETVDPIVE
     VCLSEMRSKY QCKKIGAVGY CFGGKYVVRH LIPGKMDVGY TAHPSHIDES ELKGIKGPLA
     IAAAAKDNIF PAEKRHVSEE ILQEVGFPYQ INLYSGVSHG FGVRGDMNAG EVRYAMRSAF
     VQAVEWFNEY MKEK