ID   BZLF1_EBVG              Reviewed;         245 AA.
AC   Q3KSS8; B0FNC8; B0FNC9; B0FND0; B0FND1;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Trans-activator protein BZLF1;
DE            Short=EB1;
DE   AltName: Full=Zebra;
GN   ORFNames=BZLF1;
OS   Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus.
OX   NCBI_TaxID=10376;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16306603; DOI=10.1128/jvi.79.24.15323-15330.2005;
RA   Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H.,
RA   Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.;
RT   "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a
RT   nasopharyngeal carcinoma patient.";
RL   J. Virol. 79:15323-15330(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=isolate Human/China/NPC1/2007, isolate Human/China/NPC2/2007,
RC   isolate Human/China/NPC3/2007, and isolate Human/China/NPC4/2007;
RX   PubMed=18807115; DOI=10.1007/s00705-008-0195-6;
RA   Ji K.-M., Li C.-L., Meng G., Han A.-D., Wu X.-L.;
RT   "New BZLF1 sequence variations in EBV-associated undifferentiated
RT   nasopharyngeal carcinoma in southern China.";
RL   Arch. Virol. 153:1949-1953(2008).
CC   -!- FUNCTION: Plays a key role in the switch from latent infection to lytic
CC       cycle producing new virions. Acts as a transcription factor, inducing
CC       early lytic cycle genes, and as a origin binding protein for genome
CC       replication. BZLF1 activates the promoter of another EBV gene
CC       (BSLF2+BMLF1). {ECO:0000250|UniProtKB:P03206}.
CC   -!- SUBUNIT: Homodimer. Interacts with human UBN1, CRTC2 and RACK1.
CC       Interacts (via N-terminus) with human PAX5 (via N-terminus); this
CC       interaction inhibits BZLF1-mediated lytic viral reactivation.
CC       {ECO:0000250|UniProtKB:P03206}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250|UniProtKB:P03206}.
CC   -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
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DR   EMBL; AY961628; ABA06392.1; -; Genomic_DNA.
DR   EMBL; EU340364; ABY56265.1; -; mRNA.
DR   EMBL; EU340365; ABY56266.1; -; mRNA.
DR   EMBL; EU340366; ABY56267.1; -; mRNA.
DR   EMBL; EU340367; ABY56268.1; -; mRNA.
DR   EMBL; EU340368; ABY56269.1; -; mRNA.
DR   PDB; 4JQV; X-ray; 1.50 A; B=173-180.
DR   PDB; 4JQX; X-ray; 1.90 A; B=169-180.
DR   PDB; 4JRX; X-ray; 2.30 A; C=52-64.
DR   PDB; 4JRY; X-ray; 2.80 A; C=52-64.
DR   PDBsum; 4JQV; -.
DR   PDBsum; 4JQX; -.
DR   PDBsum; 4JRX; -.
DR   PDBsum; 4JRY; -.
DR   SMR; Q3KSS8; -.
DR   Proteomes; UP000007641; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW.
DR   InterPro; IPR004827; bZIP.
DR   InterPro; IPR046347; bZIP_sf.
DR   InterPro; IPR017339; Trans-activator_BZLF1_HHV-4.
DR   PIRSF; PIRSF037966; BZLF1; 1.
DR   SUPFAM; SSF57959; SSF57959; 1.
DR   PROSITE; PS00036; BZIP_BASIC; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Early protein;
KW   G0/G1 host cell cycle checkpoint dysregulation by virus; Host nucleus;
KW   Host-virus interaction; Modulation of host cell cycle by virus;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..245
FT                   /note="Trans-activator protein BZLF1"
FT                   /id="PRO_0000408268"
FT   DOMAIN          178..228
FT                   /note="bZIP"
FT   REGION          145..167
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          178..195
FT                   /note="Basic motif"
FT                   /evidence="ECO:0000250"
FT   REGION          196..228
FT                   /note="Leucine-zipper"
FT                   /evidence="ECO:0000250"
FT   VARIANT         11
FT                   /note="V -> A (in strain: isolate Human/China/NPC2/2007,
FT                   isolate Human/China/NPC3/2007)"
FT   VARIANT         31
FT                   /note="R -> G (in strain: isolate Human/China/NPC3/2007)"
FT   VARIANT         83
FT                   /note="E -> G (in strain: isolate Human/China/NPC2/2007)"
FT   VARIANT         103
FT                   /note="N -> D (in strain: isolate Human/China/NPC3/2007)"
FT   VARIANT         108
FT                   /note="Q -> L (in strain: isolate Human/China/NPC2/2007)"
FT   VARIANT         123
FT                   /note="Q -> R (in strain: isolate Human/China/NPC2/2007)"
FT   VARIANT         127
FT                   /note="A -> V (in strain: isolate Human/China/NPC3/2007)"
FT   VARIANT         155
FT                   /note="P -> L (in strain: isolate Human/China/NPC3/2007)"
FT   VARIANT         181
FT                   /note="K -> R (in strain: isolate Human/China/NPC1/2007 and
FT                   isolate Human/China/NPC3/2007)"
FT   VARIANT         200
FT                   /note="Y -> C (in strain: isolate Human/China/NPC4/2007)"
FT   STRAND          57..59
FT                   /evidence="ECO:0007829|PDB:4JRX"
SQ   SEQUENCE   245 AA;  26813 MW;  A8D1EC1AC31DCFE7 CRC64;
     MMDPNSTSED VKFTPDPYQV PFVQAFDQAT RVYQDLGGPS QAPLPCVLWP VLPEPLPQGQ
     LTAYHVSAAP TGSWFPAPQP APENAYQAYA APQLFPVSDI TQNQLTNQAG GEAPQPGDNS
     TVQPAAAVVL ACPGANQEQQ LADIGAPQPA PAAAPARRTR KPLQPESLEE CDSELEIKRY
     KNRVASRKCR AKFKHLLQHY REVASAKSSE NDRLRLLLKQ MCPSLDVDSI IPRTPDVLHE
     DLLNF