BZP50_ORYSJ
ID BZP50_ORYSJ Reviewed; 304 AA.
AC Q69XV0;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=bZIP transcription factor 50 {ECO:0000303|PubMed:18065552};
DE Short=OsbZIP50 {ECO:0000303|PubMed:18065552};
DE AltName: Full=OsBZIP74 {ECO:0000303|PubMed:22199238};
GN Name=BZIP50 {ECO:0000303|PubMed:18065552};
GN Synonyms=BZIP60 {ECO:0000303|PubMed:21223397},
GN BZIP74 {ECO:0000303|PubMed:22199238};
GN OrderedLocusNames=Os06g0622700 {ECO:0000312|EMBL:BAF20014.1},
GN LOC_Os06g41770 {ECO:0000305};
GN ORFNames=OsJ_22012 {ECO:0000312|EMBL:EEE66035.1},
GN P0040H10.41 {ECO:0000312|EMBL:BAD35377.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18065552; DOI=10.1104/pp.107.112821;
RA Nijhawan A., Jain M., Tyagi A.K., Khurana J.P.;
RT "Genomic survey and gene expression analysis of the basic leucine zipper
RT transcription factor family in rice.";
RL Plant Physiol. 146:333-350(2008).
RN [6]
RP FUNCTION, AND INDUCTION BY DITHIOTHREITOL.
RX PubMed=21223397; DOI=10.1111/j.1365-313x.2010.04453.x;
RA Wakasa Y., Yasuda H., Oono Y., Kawakatsu T., Hirose S., Takahashi H.,
RA Hayashi S., Yang L., Takaiwa F.;
RT "Expression of ER quality control-related genes in response to changes in
RT BiP1 levels in developing rice endosperm.";
RL Plant J. 65:675-689(2011).
RN [7]
RP FUNCTION, ACTIVITY REGULATION, SUBCELLULAR LOCATION, AND ALTERNATIVE
RP SPLICING.
RX PubMed=22199238; DOI=10.1093/mp/ssr115;
RA Lu S.J., Yang Z.T., Sun L., Sun L., Song Z.T., Liu J.X.;
RT "Conservation of IRE1-regulated bZIP74 mRNA unconventional splicing in rice
RT (Oryza sativa L.) involved in ER stress responses.";
RL Mol. Plant 5:504-514(2012).
RN [8]
RP FUNCTION, ACTIVITY REGULATION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING,
RP AND INDUCTION.
RX PubMed=22050533; DOI=10.1111/j.1365-313x.2011.04844.x;
RA Hayashi S., Wakasa Y., Takahashi H., Kawakatsu T., Takaiwa F.;
RT "Signal transduction by IRE1-mediated splicing of bZIP50 and other stress
RT sensors in the endoplasmic reticulum stress response of rice.";
RL Plant J. 69:946-956(2012).
CC -!- FUNCTION: Transcription factor involved in endoplasmic reticulum (ER)
CC stress response (PubMed:21223397, PubMed:22050533, PubMed:22199238).
CC Acts downstream of the ER stress sensors IRE1, BZIP39 and BZIP60 to
CC activate BiP chaperone genes (PubMed:22050533, PubMed:22199238).
CC {ECO:0000269|PubMed:21223397, ECO:0000269|PubMed:22050533,
CC ECO:0000269|PubMed:22199238}.
CC -!- ACTIVITY REGULATION: Transcriptionally activated by IRE1 in response to
CC endoplasmic reticulum (ER) stress. IRE1 cleaves a 20-bp fragment
CC causing a frameshift of the mRNA transcript, leading to a nuclear
CC isoform of the BZIP50 activator. {ECO:0000269|PubMed:22050533,
CC ECO:0000269|PubMed:22199238}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:22050533, ECO:0000269|PubMed:22199238}; Single-pass
CC membrane protein {ECO:0000255}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Nucleus
CC {ECO:0000269|PubMed:22050533, ECO:0000269|PubMed:22199238}. Note=The
CC bZIP domain is translocated into the nucleus in response to ER stress.
CC {ECO:0000269|PubMed:22050533, ECO:0000269|PubMed:22199238}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=bZIP50u {ECO:0000303|PubMed:22050533};
CC IsoId=Q69XV0-1; Sequence=Displayed;
CC Name=2; Synonyms=bZIP50s {ECO:0000303|PubMed:22050533};
CC IsoId=Q69XV0-2; Sequence=VSP_058655;
CC -!- INDUCTION: By dithiothreitol-induced endoplasmic reticulum (ER) stress
CC response (PubMed:22050533, PubMed:21223397). Induced by salt stress
CC (PubMed:22050533). {ECO:0000269|PubMed:21223397,
CC ECO:0000269|PubMed:22050533}.
CC -!- MISCELLANEOUS: [Isoform 2]: Potent transcriptional activator. Induced
CC by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 20-
CC bp fragment causing a frameshift of the mRNA transcript.
CC {ECO:0000269|PubMed:22050533, ECO:0000269|PubMed:22199238}.
CC -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
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DR EMBL; AP003539; BAD35377.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF20014.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS98675.1; -; Genomic_DNA.
DR EMBL; CM000143; EEE66035.1; -; Genomic_DNA.
DR RefSeq; XP_015641141.1; XM_015785655.1. [Q69XV0-1]
DR AlphaFoldDB; Q69XV0; -.
DR SMR; Q69XV0; -.
DR STRING; 4530.OS06T0622700-01; -.
DR PaxDb; Q69XV0; -.
DR PRIDE; Q69XV0; -.
DR EnsemblPlants; Os06t0622700-01; Os06t0622700-01; Os06g0622700. [Q69XV0-1]
DR GeneID; 4341554; -.
DR Gramene; Os06t0622700-01; Os06t0622700-01; Os06g0622700. [Q69XV0-1]
DR KEGG; osa:4341554; -.
DR eggNOG; KOG0017; Eukaryota.
DR HOGENOM; CLU_054669_0_0_1; -.
DR InParanoid; Q69XV0; -.
DR OMA; CKASRTK; -.
DR OrthoDB; 1315234at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IMP:UniProtKB.
DR GO; GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR Pfam; PF00170; bZIP_1; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Endoplasmic reticulum; Membrane;
KW Nucleus; Reference proteome; Transcription; Transcription regulation;
KW Transmembrane; Transmembrane helix; Unfolded protein response.
FT CHAIN 1..304
FT /note="bZIP transcription factor 50"
FT /id="PRO_0000438369"
FT TOPO_DOM 1..222
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..304
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT DOMAIN 141..203
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 26..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 94..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 143..167
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 169..183
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT VAR_SEQ 218..304
FT /note="VLTETLPLVSLLWLVSIVCLLPVPGLPNRNPVARSSAGRDLATVTGKKTSSE
FT QQLEETLLLHGRRCKGSRARIKLDTGPFRLAAAAC -> AGFPALAGEHRLPVPDARSA
FT QPKPGGSKQRRKRSRDGNRKEDKQ (in isoform 2)"
FT /id="VSP_058655"
SQ SEQUENCE 304 AA; 32613 MW; 0DAE376C6256F2D8 CRC64;
MDVEFFADLD LDALLASFSS SAAAAGSGVS GLFAPSPPHD AEAGSPESVS SRRPSPSREA
ALSEIERFLM EEGPAAEEGV GAEDFFDALL VDGGEEEEEE EGKGSEAGGS TDGDSGKENE
VATPDAEKED VEAEVDGDDP MSKKKRRQMR NRDSAMKSRE RKKMYVKDLE TKSKYLEAEC
RRLSYALQCC AAENMALRQS LLKDRPVGAA TAMQESAVLT ETLPLVSLLW LVSIVCLLPV
PGLPNRNPVA RSSAGRDLAT VTGKKTSSEQ QLEETLLLHG RRCKGSRARI KLDTGPFRLA
AAAC
