BZP60_ARATH
ID BZP60_ARATH Reviewed; 295 AA.
AC Q9C7S0;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=bZIP transcription factor 60 {ECO:0000303|PubMed:11906833};
DE Short=AtbZIP60 {ECO:0000303|PubMed:11906833};
GN Name=BZIP60 {ECO:0000303|PubMed:11906833}; OrderedLocusNames=At1g42990;
GN ORFNames=F13A11.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11906833; DOI=10.1016/s1360-1385(01)02223-3;
RA Jakoby M., Weisshaar B., Droege-Laser W., Vicente-Carbajosa J.,
RA Tiedemann J., Kroj T., Parcy F.;
RT "bZIP transcription factors in Arabidopsis.";
RL Trends Plant Sci. 7:106-111(2002).
RN [5]
RP FUNCTION, INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15781873; DOI=10.1073/pnas.0408941102;
RA Iwata Y., Koizumi N.;
RT "An Arabidopsis transcription factor, AtbZIP60, regulates the endoplasmic
RT reticulum stress response in a manner unique to plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:5280-5285(2005).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18574595; DOI=10.1007/s00438-008-0356-z;
RA Lu D.-P., Christopher D.A.;
RT "Endoplasmic reticulum stress activates the expression of a sub-group of
RT protein disulfide isomerase genes and AtbZIP60 modulates the response in
RT Arabidopsis thaliana.";
RL Mol. Genet. Genomics 280:199-210(2008).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=19017746; DOI=10.1105/tpc.108.061002;
RA Iwata Y., Fedoroff N.V., Koizumi N.;
RT "Arabidopsis bZIP60 is a proteolysis-activated transcription factor
RT involved in the endoplasmic reticulum stress response.";
RL Plant Cell 20:3107-3121(2008).
RN [8]
RP INTERACTION WITH BZIP28.
RX PubMed=20207753; DOI=10.1105/tpc.109.072173;
RA Liu J.X., Howell S.H.;
RT "bZIP28 and NF-Y transcription factors are activated by ER stress and
RT assemble into a transcriptional complex to regulate stress response genes
RT in Arabidopsis.";
RL Plant Cell 22:782-796(2010).
RN [9]
RP ALTERNATIVE SPLICING, FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION BY
RP HEAT.
RX PubMed=21482766; DOI=10.1073/pnas.1102117108;
RA Deng Y., Humbert S., Liu J.X., Srivastava R., Rothstein S.J., Howell S.H.;
RT "Heat induces the splicing by IRE1 of a mRNA encoding a transcription
RT factor involved in the unfolded protein response in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:7247-7252(2011).
RN [10]
RP ALTERNATIVE SPLICING.
RX PubMed=22355548; DOI=10.1038/srep00029;
RA Nagashima Y., Mishiba K., Suzuki E., Shimada Y., Iwata Y., Koizumi N.;
RT "Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to
RT produce the active transcription factor.";
RL Sci. Rep. 1:29-29(2011).
RN [11]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22359644; DOI=10.1371/journal.pone.0031944;
RA Moreno A.A., Mukhtar M.S., Blanco F., Boatwright J.L., Moreno I.,
RA Jordan M.R., Chen Y., Brandizzi F., Dong X., Orellana A.,
RA Pajerowska-Mukhtar K.M.;
RT "IRE1/bZIP60-mediated unfolded protein response plays distinct roles in
RT plant immunity and abiotic stress responses.";
RL PLoS ONE 7:E31944-E31944(2012).
RN [12]
RP REVIEW.
RX PubMed=22796463; DOI=10.1016/j.tplants.2012.06.014;
RA Iwata Y., Koizumi N.;
RT "Plant transducers of the endoplasmic reticulum unfolded protein
RT response.";
RL Trends Plant Sci. 17:720-727(2012).
CC -!- FUNCTION: Transcription factor involved in the unfolded protein
CC response (UPR). Acts during endoplasmic reticulum stress (ER) by
CC activating unfolded protein response (UPR) target genes via direct
CC binding to the UPR element (UPRE). Plays a role in plant immunity and
CC abiotic stress responses. {ECO:0000269|PubMed:15781873,
CC ECO:0000269|PubMed:18574595, ECO:0000269|PubMed:19017746,
CC ECO:0000269|PubMed:21482766, ECO:0000269|PubMed:22359644}.
CC -!- SUBUNIT: Interacts with BZIP28. {ECO:0000269|PubMed:20207753}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Endoplasmic reticulum membrane;
CC Single-pass membrane protein.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Nucleus. Note=The bZIP domain is
CC translocated into the nucleus in response to ER stress.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=bZIP60u;
CC IsoId=Q9C7S0-1; Sequence=Displayed;
CC Name=2; Synonyms=bZIP60s;
CC IsoId=Q9C7S0-2; Sequence=VSP_046442;
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, rosette and cauline leaves,
CC stems, buds, flowers, siliques, immature seeds, anthers and pollen
CC grains. {ECO:0000269|PubMed:19017746}.
CC -!- INDUCTION: By tunicamycin, DTT and azetidine-2-carboxylate. Isoform 2
CC is induced by heat. {ECO:0000269|PubMed:15781873,
CC ECO:0000269|PubMed:21482766}.
CC -!- DOMAIN: The C-terminal transmembrane domain is cleaved in response to
CC ER stress and the N-terminal fragment containing the bZIP domain is
CC sufficient for transcription activation.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, but reduced induction of ER
CC stress-responsive genes. Shows enhanced susceptibility to a bacterial
CC pathogen. {ECO:0000269|PubMed:18574595, ECO:0000269|PubMed:19017746,
CC ECO:0000269|PubMed:22359644}.
CC -!- MISCELLANEOUS: Expression and cleavage of bZIP60 in anthers observed in
CC the absence of stress treatment, suggesting that the ER stress response
CC functions in the normal development of active secretory cells.
CC -!- MISCELLANEOUS: [Isoform 2]: Potent transcriptional activator. Induced
CC by IRE1-1 in response to endoplasmic reticulum stress. IRE1-1 cleaves a
CC 23-bp fragment causing a frameshift of the mRNA transcript.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
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DR EMBL; AC068324; AAG51519.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31935.1; -; Genomic_DNA.
DR EMBL; AY045964; AAK76638.1; -; mRNA.
DR EMBL; AY133805; AAM91739.1; -; mRNA.
DR PIR; E96497; E96497.
DR RefSeq; NP_174998.1; NM_103458.3. [Q9C7S0-1]
DR AlphaFoldDB; Q9C7S0; -.
DR SMR; Q9C7S0; -.
DR BioGRID; 26122; 9.
DR IntAct; Q9C7S0; 5.
DR STRING; 3702.AT1G42990.1; -.
DR PaxDb; Q9C7S0; -.
DR PRIDE; Q9C7S0; -.
DR EnsemblPlants; AT1G42990.1; AT1G42990.1; AT1G42990. [Q9C7S0-1]
DR GeneID; 840897; -.
DR Gramene; AT1G42990.1; AT1G42990.1; AT1G42990. [Q9C7S0-1]
DR KEGG; ath:AT1G42990; -.
DR Araport; AT1G42990; -.
DR TAIR; locus:2009874; AT1G42990.
DR eggNOG; KOG0017; Eukaryota.
DR HOGENOM; CLU_054669_1_0_1; -.
DR InParanoid; Q9C7S0; -.
DR OMA; FMFLEFQ; -.
DR OrthoDB; 1315234at2759; -.
DR PhylomeDB; Q9C7S0; -.
DR PRO; PR:Q9C7S0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C7S0; baseline and differential.
DR Genevisible; Q9C7S0; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IPI:TAIR.
DR GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; IMP:TAIR.
DR GO; GO:0002376; P:immune system process; IEA:UniProtKB-KW.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR Pfam; PF07716; bZIP_2; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Endoplasmic reticulum; Immunity;
KW Membrane; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Transmembrane; Transmembrane helix.
FT CHAIN 1..295
FT /note="bZIP transcription factor 60"
FT /id="PRO_0000376620"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 140..203
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 101..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 142..162
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 168..182
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 110..126
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 219..295
FT /note="LLLESLLLGSLLWLLGVNFICLFPYMSHTKCCLLRPEPEKLVLNGLGSSSKP
FT SYTGVSRRCKGSRPRMKYQILTLAA -> GFPALASGSKLHLPIPLYVPHKVLPPTSRT
FT RKAGSKRARE (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_046442"
SQ SEQUENCE 295 AA; 33174 MW; C3614CAD4CF76DC6 CRC64;
MAEEFGSIDL LGDEDFFFDF DPSIVIDSLP AEDFLQSSPD SWIGEIENQL MNDENHQEES
FVELDQQSVS DFIADLLVDY PTSDSGSVDL AADKVLTVDS PAAADDSGKE NSDLVVEKKS
NDSGSEIHDD DDEEGDDDAV AKKRRRRVRN RDAAVRSRER KKEYVQDLEK KSKYLERECL
RLGRMLECFV AENQSLRYCL QKGNGNNTTM MSKQESAVLL LESLLLGSLL WLLGVNFICL
FPYMSHTKCC LLRPEPEKLV LNGLGSSSKP SYTGVSRRCK GSRPRMKYQI LTLAA
