ID   TRPA_METTM              Reviewed;         265 AA.
AC   P26920; D9PUF0;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Tryptophan synthase alpha chain {ECO:0000255|HAMAP-Rule:MF_00131};
DE            EC=4.2.1.20 {ECO:0000255|HAMAP-Rule:MF_00131};
GN   Name=trpA {ECO:0000255|HAMAP-Rule:MF_00131};
GN   OrderedLocusNames=MTBMA_c02390;
OS   Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM
OS   14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium
OS   thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=79929;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 /
RC   Marburg;
RX   PubMed=1860817; DOI=10.1128/jb.173.16.5017-5023.1991;
RA   Meile L., Stettler R., Banholzer R., Kotik M., Leisinger T.;
RT   "Tryptophan gene cluster of Methanobacterium thermoautotrophicum Marburg:
RT   molecular cloning and nucleotide sequence of a putative trpEGCFBAD
RT   operon.";
RL   J. Bacteriol. 173:5017-5023(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 /
RC   Marburg;
RX   PubMed=20802048; DOI=10.1128/jb.00844-10;
RA   Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H.,
RA   Gottschalk G., Thauer R.K.;
RT   "Complete genome sequence of Methanothermobacter marburgensis, a
RT   methanoarchaeon model organism.";
RL   J. Bacteriol. 192:5850-5851(2010).
CC   -!- FUNCTION: The alpha subunit is responsible for the aldol cleavage of
CC       indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_00131}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-
CC         glyceraldehyde 3-phosphate + H2O + L-tryptophan;
CC         Xref=Rhea:RHEA:10532, ChEBI:CHEBI:15377, ChEBI:CHEBI:33384,
CC         ChEBI:CHEBI:57912, ChEBI:CHEBI:58866, ChEBI:CHEBI:59776; EC=4.2.1.20;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00131};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC       tryptophan from chorismate: step 5/5. {ECO:0000255|HAMAP-
CC       Rule:MF_00131}.
CC   -!- SUBUNIT: Tetramer of two alpha and two beta chains. {ECO:0000255|HAMAP-
CC       Rule:MF_00131}.
CC   -!- SIMILARITY: Belongs to the TrpA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00131}.
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DR   EMBL; M65060; AAA73033.1; -; Genomic_DNA.
DR   EMBL; CP001710; ADL57848.1; -; Genomic_DNA.
DR   RefSeq; WP_013295075.1; NC_014408.1.
DR   AlphaFoldDB; P26920; -.
DR   SMR; P26920; -.
DR   STRING; 79929.MTBMA_c02390; -.
DR   EnsemblBacteria; ADL57848; ADL57848; MTBMA_c02390.
DR   GeneID; 9703945; -.
DR   KEGG; mmg:MTBMA_c02390; -.
DR   PATRIC; fig|79929.8.peg.235; -.
DR   HOGENOM; CLU_016734_0_2_2; -.
DR   OMA; LVMTYWN; -.
DR   OrthoDB; 65368at2157; -.
DR   UniPathway; UPA00035; UER00044.
DR   Proteomes; UP000000345; Chromosome.
DR   GO; GO:0004834; F:tryptophan synthase activity; IEA:UniProtKB-UniRule.
DR   CDD; cd04724; Tryptophan_synthase_alpha; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00131; Trp_synth_alpha; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   InterPro; IPR018204; Trp_synthase_alpha_AS.
DR   InterPro; IPR002028; Trp_synthase_suA.
DR   PANTHER; PTHR43406; PTHR43406; 1.
DR   Pfam; PF00290; Trp_syntA; 1.
DR   SUPFAM; SSF51366; SSF51366; 1.
DR   TIGRFAMs; TIGR00262; trpA; 1.
DR   PROSITE; PS00167; TRP_SYNTHASE_ALPHA; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Lyase;
KW   Tryptophan biosynthesis.
FT   CHAIN           1..265
FT                   /note="Tryptophan synthase alpha chain"
FT                   /id="PRO_0000098894"
FT   ACT_SITE        45
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00131"
FT   ACT_SITE        56
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00131"
SQ   SEQUENCE   265 AA;  28803 MW;  13C81F7286484DA3 CRC64;
     MNYAEMFSRV EGCAFVPFVV AGDPDMETSV EIIRTLVDAG ADALEIGFPF SDPIADGTSV
     QEADLRALNS GMTTDDCFRL IERIREFTSV PVGLLVYYNL IYRMGADEFY RRAAEAGVTG
     ILAADLPPEE AGDALRAAER YGIDQIFIVA PTTDNQRLKM ISEVSSGFHY LVSVMGVTGA
     RSRVEESTLE LIERVKGAGS LPVMVGFGVS QPEHVRMLAD AGADGVIVGS AIIDLISKNL
     DDRERMLQEI YEMVRKMKTA AGGSR