TRPB2_PYRAB
ID TRPB2_PYRAB Reviewed; 440 AA.
AC Q9V150; G8ZJ44;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2012, sequence version 2.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Tryptophan synthase beta chain 2;
DE EC=4.2.1.20;
GN Name=trpB2; Synonyms=trpB-2; OrderedLocusNames=PYRAB05790;
GN ORFNames=PAB1970;
OS Pyrococcus abyssi (strain GE5 / Orsay).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=272844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic archaeon
RT Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=GE5 / Orsay;
RX PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA Gao J., Wang J.;
RT "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT Pyrococcus furiosus DSM 3638.";
RL Curr. Microbiol. 64:118-129(2012).
CC -!- FUNCTION: The beta subunit is responsible for the synthesis of L-
CC tryptophan from indole and L-serine. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-
CC glyceraldehyde 3-phosphate + H2O + L-tryptophan;
CC Xref=Rhea:RHEA:10532, ChEBI:CHEBI:15377, ChEBI:CHEBI:33384,
CC ChEBI:CHEBI:57912, ChEBI:CHEBI:58866, ChEBI:CHEBI:59776; EC=4.2.1.20;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000250};
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 5/5.
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TrpB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB49501.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ248284; CAB49501.1; ALT_INIT; Genomic_DNA.
DR EMBL; HE613800; CCE69971.1; -; Genomic_DNA.
DR PIR; F75177; F75177.
DR RefSeq; WP_048146581.1; NC_000868.1.
DR AlphaFoldDB; Q9V150; -.
DR SMR; Q9V150; -.
DR STRING; 272844.PAB1970; -.
DR EnsemblBacteria; CAB49501; CAB49501; PAB1970.
DR GeneID; 1495484; -.
DR KEGG; pab:PAB1970; -.
DR PATRIC; fig|272844.11.peg.617; -.
DR eggNOG; arCOG01432; Archaea.
DR HOGENOM; CLU_042858_1_0_2; -.
DR OrthoDB; 24741at2157; -.
DR UniPathway; UPA00035; UER00044.
DR Proteomes; UP000000810; Chromosome.
DR Proteomes; UP000009139; Chromosome.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0004834; F:tryptophan synthase activity; IEA:UniProtKB-UniRule.
DR CDD; cd06446; Trp-synth_B; 1.
DR Gene3D; 3.40.50.1100; -; 2.
DR HAMAP; MF_00133; Trp_synth_beta; 1.
DR InterPro; IPR001926; PLP-dep.
DR InterPro; IPR006316; Trp_synth_b-like.
DR InterPro; IPR006653; Trp_synth_b_CS.
DR InterPro; IPR006654; Trp_synth_beta.
DR InterPro; IPR023026; Trp_synth_beta/beta-like.
DR InterPro; IPR036052; Trypto_synt_PLP_dependent.
DR PANTHER; PTHR48077:SF6; PTHR48077:SF6; 1.
DR Pfam; PF00291; PALP; 1.
DR PIRSF; PIRSF001413; Trp_syn_beta; 1.
DR PIRSF; PIRSF500824; TrpB_prok; 1.
DR SUPFAM; SSF53686; SSF53686; 1.
DR TIGRFAMs; TIGR01415; trpB_rel; 1.
DR PROSITE; PS00168; TRP_SYNTHASE_BETA; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Lyase;
KW Pyridoxal phosphate; Tryptophan biosynthesis.
FT CHAIN 1..440
FT /note="Tryptophan synthase beta chain 2"
FT /id="PRO_0000099048"
FT MOD_RES 110
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 440 AA; 48943 MW; 580BC102EBAB36F5 CRC64;
MKVVLPDGRI PRRWYNILPD LPEPLDPPLD PETEEPIDIE KLKRIFAEEL VKQEISRERY
IEIPGELRKL YSKIGRPTPL FRATNLEKLL GTPARIYFKY EGATVTGSHK INTALAQAYY
AKKQGIERLV TETGAGQWGT ALSLAGALLG LKVRVYMARA SYQQKPYRKT LMRIYGAEVF
PSPSENTEVG KRFLKEDPNH PGSLGIAISE AIEDVLKDEK ARYSLGSVLN HVLMHQTVIG
LEAKEQMEEF EEPDVIIGCV GGGSNFAGLA YPFVKDVLDG KSEYEFIAVE PKAAPTMTRG
VYTYDYGDSA GLTPKLKMHT LGHRYYVPPI HAGGLRYHGL APTLSVLINH GIVKPIAYHQ
TEVFEAAVLF AKAEGIVPAP ESAHAVKAVI DKALEARREG KEMVILFNLS GHGLLDLKGY
EDYLDGKLED YEPRDLPVKS
