TRPB_HELPY
ID TRPB_HELPY Reviewed; 393 AA.
AC P56142;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Tryptophan synthase beta chain;
DE EC=4.2.1.20;
GN Name=trpB; OrderedLocusNames=HP_1278;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
CC -!- FUNCTION: The beta subunit is responsible for the synthesis of L-
CC tryptophan from indole and L-serine. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-
CC glyceraldehyde 3-phosphate + H2O + L-tryptophan;
CC Xref=Rhea:RHEA:10532, ChEBI:CHEBI:15377, ChEBI:CHEBI:33384,
CC ChEBI:CHEBI:57912, ChEBI:CHEBI:58866, ChEBI:CHEBI:59776; EC=4.2.1.20;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000250};
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 5/5.
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TrpB family. {ECO:0000305}.
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DR EMBL; AE000511; AAD08323.1; -; Genomic_DNA.
DR PIR; F64679; F64679.
DR RefSeq; NP_208070.1; NC_000915.1.
DR RefSeq; WP_001034280.1; NC_018939.1.
DR AlphaFoldDB; P56142; -.
DR SMR; P56142; -.
DR DIP; DIP-3612N; -.
DR IntAct; P56142; 3.
DR MINT; P56142; -.
DR STRING; 85962.C694_06605; -.
DR PaxDb; P56142; -.
DR EnsemblBacteria; AAD08323; AAD08323; HP_1278.
DR KEGG; hpy:HP_1278; -.
DR PATRIC; fig|85962.47.peg.1371; -.
DR eggNOG; COG0133; Bacteria.
DR OMA; HGMKSYF; -.
DR PhylomeDB; P56142; -.
DR UniPathway; UPA00035; UER00044.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004834; F:tryptophan synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000162; P:tryptophan biosynthetic process; IBA:GO_Central.
DR CDD; cd06446; Trp-synth_B; 1.
DR Gene3D; 3.40.50.1100; -; 2.
DR HAMAP; MF_00133; Trp_synth_beta; 1.
DR InterPro; IPR001926; PLP-dep.
DR InterPro; IPR006653; Trp_synth_b_CS.
DR InterPro; IPR006654; Trp_synth_beta.
DR InterPro; IPR023026; Trp_synth_beta/beta-like.
DR InterPro; IPR036052; Trypto_synt_PLP_dependent.
DR Pfam; PF00291; PALP; 1.
DR PIRSF; PIRSF001413; Trp_syn_beta; 1.
DR SUPFAM; SSF53686; SSF53686; 1.
DR TIGRFAMs; TIGR00263; trpB; 1.
DR PROSITE; PS00168; TRP_SYNTHASE_BETA; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Lyase;
KW Pyridoxal phosphate; Reference proteome; Tryptophan biosynthesis.
FT CHAIN 1..393
FT /note="Tryptophan synthase beta chain"
FT /id="PRO_0000098955"
FT MOD_RES 85
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 393 AA; 42684 MW; 3370A1BCD5B89371 CRC64;
MNKKAYFGEF GGSFVSELLV PALRELEQAF DACLKDEKFQ KEYFRLLKDF VGRPSPLTLC
QNIVSNPKVK LYLKREDLIH GGAHKTNQAL GQALLAKKMG KTRIIAETGA GQHGVATAIA
CALLNLKCVV FMGSKDIKRQ EMNVFRMHLL GAEVREVNSG SATLKDAVNE ALRDWASSYK
DTHYLLGTAA GPHPYPTMVK TFQKMIGDEV KSQILEKENR LPDYVIACVG GGSNAIGIFS
AFLNDKEVKL IGVEPAGLGL ETNKHGATLN KGRVGILHGN KTYLLQDDEG QIAESHSISA
GLDYPGVGPE HSYLKESGRA VYESASDAEA LEAFKLLCQK EGIIPALESS HALAYALKLA
QKCEEESIIV VNLSGRGDKD LSTVYNALKG GLK