TRPC2_RAT
ID TRPC2_RAT Reviewed; 1170 AA.
AC Q9R283; B5DFD7; O70485;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Short transient receptor potential channel 2;
DE Short=TrpC2;
DE AltName: Full=Transient receptor protein 2;
DE Short=TRP-2;
DE Short=rTrp2;
GN Name=Trpc2; Synonyms=Trp2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=10318963; DOI=10.1073/pnas.96.10.5791;
RA Liman E.R., Corey D.P., Dulac C.;
RT "TRP2: a candidate transduction channel for mammalian pheromone sensory
RT signaling.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:5791-5796(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 867-930 (ISOFORM 1/2).
RC STRAIN=Wistar; TISSUE=Heart;
RA Koizumi S., Ripley S.J., Bootman M.D., Lipp P., Berridge M.J.,
RA Bobanovic L.K.;
RT "Expression patterns of mammalian trp homologues in rat excitable cells.";
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Thought to form a receptor-activated calcium permeant cation
CC channel. Probably is operated by a phosphatidylinositol second
CC messenger system activated by receptor tyrosine kinases or G-protein
CC coupled receptors. Is not activated by intracellular calcium store
CC depletion.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9R283-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9R283-2; Sequence=VSP_037236, VSP_037237;
CC Name=3;
CC IsoId=Q9R283-3; Sequence=VSP_038807, VSP_037238, VSP_037239;
CC -!- TISSUE SPECIFICITY: Expressed exclusively in vomeronasal organ neurons
CC (sensory microvilli).
CC -!- MISCELLANEOUS: [Isoform 1]: Inferred from mouse sequence.
CC -!- SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. STrpC
CC subfamily. TRPC2 sub-subfamily. {ECO:0000305}.
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DR EMBL; AF136401; AAD31453.1; -; mRNA.
DR EMBL; AABR03002379; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03010502; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03010734; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03001253; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC169022; AAI69022.1; -; mRNA.
DR EMBL; AF061874; AAC16726.1; -; mRNA.
DR RefSeq; NP_001316819.1; NM_001329890.1. [Q9R283-3]
DR RefSeq; XP_006223495.1; XM_006223433.3. [Q9R283-3]
DR RefSeq; XP_006229966.1; XM_006229904.3. [Q9R283-3]
DR AlphaFoldDB; Q9R283; -.
DR SMR; Q9R283; -.
DR STRING; 10116.ENSRNOP00000047354; -.
DR PaxDb; Q9R283; -.
DR PRIDE; Q9R283; -.
DR Ensembl; ENSRNOT00000045726; ENSRNOP00000042378; ENSRNOG00000020188. [Q9R283-2]
DR Ensembl; ENSRNOT00000048172; ENSRNOP00000047354; ENSRNOG00000020188. [Q9R283-1]
DR GeneID; 102549471; -.
DR KEGG; rno:102549471; -.
DR UCSC; RGD:628819; rat. [Q9R283-1]
DR CTD; 102443350; -.
DR RGD; 628819; Trpc2.
DR eggNOG; KOG3609; Eukaryota.
DR GeneTree; ENSGT01050000244831; -.
DR HOGENOM; CLU_005716_2_0_1; -.
DR InParanoid; Q9R283; -.
DR OMA; MFMFILM; -.
DR PhylomeDB; Q9R283; -.
DR PRO; PR:Q9R283; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000020188; Expressed in testis and 16 other tissues.
DR ExpressionAtlas; Q9R283; baseline and differential.
DR Genevisible; Q9R283; RN.
DR GO; GO:0034703; C:cation channel complex; IBA:GO_Central.
DR GO; GO:0032590; C:dendrite membrane; ISO:RGD.
DR GO; GO:0012505; C:endomembrane system; ISO:RGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:RGD.
DR GO; GO:0000139; C:Golgi membrane; ISO:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0019992; F:diacylglycerol binding; ISO:RGD.
DR GO; GO:0070679; F:inositol 1,4,5 trisphosphate binding; ISO:RGD.
DR GO; GO:0015279; F:store-operated calcium channel activity; ISO:RGD.
DR GO; GO:0007340; P:acrosome reaction; ISO:RGD.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006816; P:calcium ion transport; ISO:RGD.
DR GO; GO:0008050; P:female courtship behavior; ISO:RGD.
DR GO; GO:0002121; P:inter-male aggressive behavior; ISO:RGD.
DR GO; GO:0006828; P:manganese ion transport; IBA:GO_Central.
DR GO; GO:0048047; P:mating behavior, sex discrimination; ISO:RGD.
DR GO; GO:1902436; P:negative regulation of male mating behavior; ISO:RGD.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:RGD.
DR GO; GO:0051480; P:regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; ISO:RGD.
DR GO; GO:0019236; P:response to pheromone; ISO:RGD.
DR GO; GO:0007338; P:single fertilization; IBA:GO_Central.
DR GO; GO:0000012; P:single strand break repair; IEA:InterPro.
DR GO; GO:0002124; P:territorial aggressive behavior; ISO:RGD.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR013555; TRP_dom.
DR InterPro; IPR005458; TRPC2_channel.
DR InterPro; IPR002153; TRPC_channel.
DR InterPro; IPR002706; Xrcc1_N.
DR PANTHER; PTHR10117; PTHR10117; 1.
DR PANTHER; PTHR10117:SF6; PTHR10117:SF6; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR Pfam; PF08344; TRP_2; 1.
DR Pfam; PF01834; XRCC1_N; 1.
DR PRINTS; PR01097; TRNSRECEPTRP.
DR PRINTS; PR01643; TRPCHANNEL2.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ANK repeat; Calcium; Calcium channel;
KW Calcium transport; Coiled coil; Ion channel; Ion transport; Membrane;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1170
FT /note="Short transient receptor potential channel 2"
FT /id="PRO_0000215308"
FT TOPO_DOM 1..627
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 628..648
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 649..658
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 659..679
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 680..701
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 702..722
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 723..737
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 738..758
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 759..788
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 789..809
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 810..832
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 833..853
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 854..898
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 899..919
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 920..1170
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REPEAT 300..329
FT /note="ANK 1"
FT REPEAT 346..376
FT /note="ANK 2"
FT REPEAT 377..405
FT /note="ANK 3"
FT REPEAT 429..458
FT /note="ANK 4"
FT REGION 64..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 142..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 322..342
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1118..1170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1030..1068
FT /evidence="ECO:0000255"
FT COMPBIAS 66..113
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 160..180
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 194..231
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1130..1145
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1146..1170
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..283
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10318963"
FT /id="VSP_037236"
FT VAR_SEQ 1
FT /note="M -> MAPVKISHVVSFSSQDPKYPVENLLNPDSHKGPWLSCPRDKTGQLKV
FT EFQLERAVPISYIDVGNCGCAFLQIDVGRSSWPLDRPFVTLLPATM (in isoform
FT 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_038807"
FT VAR_SEQ 274..315
FT /note="RCLHVALTPVPLIPKPNWTEIVNKKLKFPPTLLRAIQEGQLG -> WVSSPE
FT SSPPPSSWVQCPICELQFSAREIEEHASVCGEVFPA (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_037238"
FT VAR_SEQ 284..288
FT /note="PLIPK -> MDPLS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10318963"
FT /id="VSP_037237"
FT VAR_SEQ 316..1170
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_037239"
FT CONFLICT 1010
FT /note="H -> R (in Ref. 1; AAD31453)"
FT /evidence="ECO:0000305"
FT CONFLICT 1069..1070
FT /note="Missing (in Ref. 1; AAD31453)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1170 AA; 130467 MW; BDF876A83B28CA5C CRC64;
MLMSLTDSKE GKNRSGVRMF KDDDFLIPAS GESWDRLRLT CSQPFTRHQS FGLAFLRVRS
SLDSLSDPVK DPSSPGSSGL NQNSSDKLES DPSPWLTNPS IRRTFFPDPQ TSTKEISALK
GMLKQLQPGP LGRAARMVLS AAHKAPPASV VSPNNSHGEP DSSHPERAEP RAEEPNRKNN
ASRGKRRKVQ EQRRPLSSSS SQPNRRATGR TKQRQQRPQA KSDGSGVQAT GQCPICTGSF
SIEALPRHAA TCGESSPPQP ASPTSLSSSE SVLRCLHVAL TPVPLIPKPN WTEIVNKKLK
FPPTLLRAIQ EGQLGLVQQL LESGSDPSGA GPGGPLRNVE ESEDRSWREA LNLAIRLGHE
VITDVLLANV KFDFRQIHEA LLVAVDTNQP AVVRRLLARL EREKGRKVDT KSFSLAFFDS
SIDGSRFAPG VTPLTLACQK DLYEIAQLLM DQGHTIARPH PVSCACLECS NARRYDLLKF
SLSRINTYRG IASRAHLSLA SEDAMLAAFQ LSRELRRLAR KEPEFKPQYI ALESLCQDYG
FELLGMCRNQ SEVTAVLNDL GEDSETEPEA EGLGQAFEEG IPNLARLRLA VNYNQKQFVA
HPICQQVLSS IWCGNLAGWR GSTTIWKLFV AFLIFLTMPF LCIGYWLAPK SRLGRLLKIP
VLKFLLHSAS YLWFLIFLLG ESLVMETQLS TFKGRSQSVW ETSLHMIWVT GFLWFECKEV
WIEGLRSYLL DWWNFLDVVI LSLYLASFAL RLLLAGLAYM HCRDASDSST CRYFTTAERS
EWRTEDPQFL AEVLFAVTSM LSFTRLAYIL PAHESLGTLQ ISIGKMIDDM IRFMFILMII
LTAFLCGLNN IYVPYQETEK LGNFNETFQF LFWTMFGMEE HSVVDMPQFL VPEFVGRAMY
GIFTIVMVIV LLNMLIAMIT NSFQKIEDDA DVEWKFARSK LYLSYFREGL TLPVPFNILP
SPKAAFYLLR RIFRFICCGS SCCKAKKSDY PPIPTFTNPG ARAGPGEGEH VSYRLRVIKA
LVQRYIETAR REFEETRRKD LGNRLTELTK TVSRLQSEVA SVQKTVAAGG ALRPPDGASI
LSRYITRVRN SFQNLGPPAP DTPAELTMPG IVETEVSLED SLDATGEAGT PASGESSSSS
SAHVLVHREQ EAEGAGDLPL GEDLETKGES
