TRPC3_MOUSE
ID TRPC3_MOUSE Reviewed; 910 AA.
AC Q9QZC1; B1ATV3; Q61058;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 03-AUG-2022, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Short transient receptor potential channel 3;
DE Short=TrpC3;
DE AltName: Full=Receptor-activated cation channel TRP3;
DE AltName: Full=Transient receptor protein 3;
DE Short=TRP-3;
DE Short=mTrp3;
DE AltName: Full=Trp-related protein 3;
GN Name=Trpc3; Synonyms=Trp3, Trrp3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 75-910.
RC TISSUE=Brain;
RX PubMed=9512398;
RA Mori Y., Takada N., Okada T., Wakamori M., Imoto K., Wanifuchi H., Oka H.,
RA Oba A., Ikenaka K., Kurosaki T.;
RT "Differential distribution of TRP Ca2+ channel isoforms in mouse brain.";
RL NeuroReport 9:507-515(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 637-745.
RX PubMed=8646775; DOI=10.1016/s0092-8674(00)81233-7;
RA Zhu X., Jiang M., Peyton M., Boulay G., Hurst R., Stefani E.,
RA Birnbaumer L.;
RT "trp, a novel mammalian gene family essential for agonist-activated
RT capacitative Ca2+ entry.";
RL Cell 85:661-671(1996).
CC -!- FUNCTION: Thought to form a receptor-activated non-selective calcium
CC permeant cation channel. Probably is operated by a phosphatidylinositol
CC second messenger system activated by receptor tyrosine kinases or G-
CC protein coupled receptors. Activated by diacylglycerol (DAG) in a
CC membrane-delimited fashion, independently of protein kinase C, and by
CC inositol 1,4,5-triphosphate receptors (ITPR) with bound IP3 (By
CC similarity). May also be activated by internal calcium store depletion.
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ITPR1, ITPR3, MX1 and RNF24 (By similarity).
CC Interacts with JPH2; the interaction is involved in maintaining Ca(2+)
CC homeostasis in skeletal muscle and is mediated by JPH2 'Ser-165'
CC phosphorylation (By similarity). {ECO:0000250|UniProtKB:Q13507}.
CC -!- INTERACTION:
CC Q9QZC1; Q13563: PKD2; Xeno; NbExp=3; IntAct=EBI-10051366, EBI-7813714;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Abundantly expressed in brain. Concentrated in
CC cerebellar Purkinje cells and sparsely localized in cerebellar granule
CC lyer, pontine nuclei and thalamus. Lower levels detected in other
CC tissues.
CC -!- SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. STrpC
CC subfamily. TRPC3 sub-subfamily. {ECO:0000305}.
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DR EMBL; AL663106; CAM28054.1; -; Genomic_DNA.
DR EMBL; AF190645; AAF01468.1; -; mRNA.
DR EMBL; U40982; AAC52701.1; -; mRNA.
DR RefSeq; NP_062383.2; NM_019510.2.
DR AlphaFoldDB; Q9QZC1; -.
DR SMR; Q9QZC1; -.
DR DIP; DIP-61309N; -.
DR IntAct; Q9QZC1; 4.
DR STRING; 10090.ENSMUSP00000029271; -.
DR BindingDB; Q9QZC1; -.
DR ChEMBL; CHEMBL4739706; -.
DR GlyGen; Q9QZC1; 1 site.
DR iPTMnet; Q9QZC1; -.
DR PhosphoSitePlus; Q9QZC1; -.
DR MaxQB; Q9QZC1; -.
DR PaxDb; Q9QZC1; -.
DR PRIDE; Q9QZC1; -.
DR ProteomicsDB; 297521; -.
DR Antibodypedia; 15891; 287 antibodies from 34 providers.
DR DNASU; 22065; -.
DR Ensembl; ENSMUST00000029271.5; ENSMUSP00000029271.5; ENSMUSG00000027716.14.
DR GeneID; 22065; -.
DR KEGG; mmu:22065; -.
DR UCSC; uc008ozt.2; mouse.
DR CTD; 7222; -.
DR MGI; MGI:109526; Trpc3.
DR VEuPathDB; HostDB:ENSMUSG00000027716; -.
DR eggNOG; KOG3609; Eukaryota.
DR GeneTree; ENSGT01050000244831; -.
DR HOGENOM; CLU_005716_4_2_1; -.
DR InParanoid; Q9QZC1; -.
DR OMA; IDVWASK; -.
DR OrthoDB; 1018075at2759; -.
DR TreeFam; TF313147; -.
DR Reactome; R-MMU-114508; Effects of PIP2 hydrolysis.
DR Reactome; R-MMU-139853; Elevation of cytosolic Ca2+ levels.
DR Reactome; R-MMU-3295583; TRP channels.
DR BioGRID-ORCS; 22065; 0 hits in 63 CRISPR screens.
DR ChiTaRS; Trpc3; mouse.
DR PRO; PR:Q9QZC1; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q9QZC1; protein.
DR Bgee; ENSMUSG00000027716; Expressed in cerebellum lobe and 132 other tissues.
DR GO; GO:0034703; C:cation channel complex; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0005227; F:calcium activated cation channel activity; ISO:MGI.
DR GO; GO:0070679; F:inositol 1,4,5 trisphosphate binding; ISO:MGI.
DR GO; GO:0015279; F:store-operated calcium channel activity; IBA:GO_Central.
DR GO; GO:0070588; P:calcium ion transmembrane transport; ISO:MGI.
DR GO; GO:0006828; P:manganese ion transport; IBA:GO_Central.
DR GO; GO:0010524; P:positive regulation of calcium ion transport into cytosol; ISO:MGI.
DR GO; GO:1903244; P:positive regulation of cardiac muscle hypertrophy in response to stress; ISO:MGI.
DR GO; GO:0051480; P:regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0033198; P:response to ATP; ISO:MGI.
DR GO; GO:0051592; P:response to calcium ion; ISO:MGI.
DR GO; GO:0007338; P:single fertilization; IBA:GO_Central.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR013555; TRP_dom.
DR InterPro; IPR005459; TRPC3_channel.
DR InterPro; IPR002153; TRPC_channel.
DR PANTHER; PTHR10117; PTHR10117; 1.
DR PANTHER; PTHR10117:SF8; PTHR10117:SF8; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR Pfam; PF08344; TRP_2; 1.
DR PRINTS; PR01097; TRNSRECEPTRP.
DR PRINTS; PR01644; TRPCHANNEL3.
DR SMART; SM00248; ANK; 3.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Calcium; Calcium channel; Calcium transport; Cell membrane;
KW Glycoprotein; Ion channel; Ion transport; Membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..910
FT /note="Short transient receptor potential channel 3"
FT /id="PRO_0000215311"
FT TOPO_DOM 1..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 465..492
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 514..525
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 526..546
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 547..597
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 598..618
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 619..641
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 642..662
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 663..711
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 712..732
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 733..910
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REPEAT 100..129
FT /note="ANK 1"
FT /evidence="ECO:0000255"
FT REPEAT 135..164
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT REPEAT 166..192
FT /note="ANK 3"
FT /evidence="ECO:0000255"
FT REPEAT 221..250
FT /note="ANK 4"
FT /evidence="ECO:0000255"
FT REPEAT 623..652
FT /note="ANK 5"
FT /evidence="ECO:0000255"
FT CARBOHYD 465
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 478
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 251
FT /note="P -> A (in Ref. 2; AAF01468)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 910 AA; 103620 MW; 3773375324F0E67C CRC64;
MSTKVKKCRE PARVTLPAPE EEEDGEAEGG ESQRRRRGWR GVNGGLEPPC PRAPPSPGPD
ASSEGSPSRW RTAGMRDKGR RQAVRGPAFM FGARGPSLTA EEERFLDAAE YGNIPVVRKM
LEESRTLNVN CVDYMGQNAL QLAVGNEHLE VTELLLKKEN LARIGDALLL AISKGYVRIV
EAILGHPGFA ASRRLTLSPC EQELRDDDFY AYDEDGTRFS PDITPIILAA HCHKYEVVHL
LLLKGARIER PHDYFCRCSD CAEKQRLDAF SHSRSRINAY KGLASPAYLS LSSEDPVLTA
LELSNELAKL ANIEKEFKND YRKLSMQCKD FVVGVLDLCR DSEEVEAILN GDLESAEPLE
RHGHKASLSR VKLAIKYEVK KFVAHPNCQQ QLLTIWYENL SGLREQTIAI KCLVVLVVAL
GLPFLAIGYW IAPCSRLGKI LRSPFMKFVA HAASFIIFLG LLVFNASDRF EGITTLPNIT
VIDYPKQIFR VKTTQFTWTE MLIMVWVLGM MWSECKELWL EGPREYIVQL WNVLDFGMLS
IFIAAFTARF LAFLQATKAQ QYVDSHVQES DLSEVTLPPE VQYFTYARDK WLPSDPQIIS
EGLYAIAVVL SFSRIAYILP ANESFGPLQI SLGRTVKDIF KFMVLFIMVF LAFMIGMFIL
YSYYLGAKVN PAFTTVEESF KTLFWSIFGL SEVTSVVLKY DHKFIENIGY VLYGIYNVTM
VVVLLNMLIA MINSSYQEIE DDSDVEWKFA RSKLWLSYFD DGKTLPPPFS LVPSPKSFVY
FIMRITNFSK CRRRRLQKDL ELGMGNSKSR LNLFTQSNSR VFESHSFNSI LNQPTRYQQI
MKRLIKRYVL KAQVDKENDE VNEGELKEIK QDISSLRYEL LEDKSQATEE LAILIHKLSE
KLNPSVLRCE
