TRPC_BACSU
ID TRPC_BACSU Reviewed; 249 AA.
AC P03964;
DT 23-OCT-1986, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 3.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Indole-3-glycerol phosphate synthase;
DE Short=IGPS;
DE EC=4.1.1.48;
GN Name=trpC; OrderedLocusNames=BSU22660;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3924737; DOI=10.1016/0378-1119(85)90125-8;
RA Henner D.J., Band L., Shimotsu H.;
RT "Nucleotide sequence of the Bacillus subtilis tryptophan operon.";
RL Gene 34:169-177(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION.
RX PubMed=10568751; DOI=10.1101/gr.9.11.1116;
RA Medigue C., Rose M., Viari A., Danchin A.;
RT "Detecting and analyzing DNA sequencing errors: toward a higher quality of
RT the Bacillus subtilis genome sequence.";
RL Genome Res. 9:1116-1127(1999).
RN [4]
RP SEQUENCE REVISION TO 33 AND 110.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-245.
RX PubMed=6425119; DOI=10.1016/0378-1119(84)90238-5;
RA Band L., Shimotsu H., Henner D.J.;
RT "Nucleotide sequence of the Bacillus subtilis trpE and trpD genes.";
RL Gene 27:55-65(1984).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+)
CC = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O;
CC Xref=Rhea:RHEA:23476, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:58613, ChEBI:CHEBI:58866; EC=4.1.1.48;
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 4/5.
CC -!- SIMILARITY: Belongs to the TrpC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; K01391; AAA22867.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14182.3; -; Genomic_DNA.
DR PIR; A01134; GWBS.
DR RefSeq; NP_390147.3; NC_000964.3.
DR RefSeq; WP_010886553.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P03964; -.
DR SMR; P03964; -.
DR PRIDE; P03964; -.
DR KEGG; bsu:BSU22660; -.
DR InParanoid; P03964; -.
DR OMA; RGPHDLI; -.
DR PhylomeDB; P03964; -.
DR BioCyc; BSUB:BSU22660-MON; -.
DR SABIO-RK; P03964; -.
DR UniPathway; UPA00035; UER00043.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0004425; F:indole-3-glycerol-phosphate synthase activity; IBA:GO_Central.
DR GO; GO:0004640; F:phosphoribosylanthranilate isomerase activity; IBA:GO_Central.
DR GO; GO:0000162; P:tryptophan biosynthetic process; IBA:GO_Central.
DR CDD; cd00331; IGPS; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00134_B; IGPS_B; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR045186; Indole-3-glycerol_P_synth.
DR InterPro; IPR013798; Indole-3-glycerol_P_synth_dom.
DR InterPro; IPR001468; Indole-3-GlycerolPSynthase_CS.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR PANTHER; PTHR22854; PTHR22854; 1.
DR Pfam; PF00218; IGPS; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR PROSITE; PS00614; IGPS; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Decarboxylase;
KW Lyase; Reference proteome; Tryptophan biosynthesis.
FT CHAIN 1..249
FT /note="Indole-3-glycerol phosphate synthase"
FT /id="PRO_0000154213"
FT CONFLICT 4
FT /note="K -> N (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 20
FT /note="V -> E (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 36
FT /note="P -> L (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="D -> N (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 64
FT /note="Q -> R (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="A -> T (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 74..78
FT /note="ADAIS -> RMRFP (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 74
FT /note="A -> P (in Ref. 1; AAA22867)"
FT /evidence="ECO:0000305"
FT CONFLICT 77
FT /note="I -> L (in Ref. 1; AAA22867)"
FT /evidence="ECO:0000305"
FT CONFLICT 110
FT /note="I -> II (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 149
FT /note="G -> E (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 160
FT /note="S -> T (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 176
FT /note="V -> I (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 198
FT /note="V -> A (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 220
FT /note="N -> K (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="R -> Q (in Ref. 1; AAA22867 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 248
FT /note="R -> G (in Ref. 1; AAA22867)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 249 AA; 27741 MW; B9F9FC8EB1C62F7B CRC64;
MLEKIIKQKK EEVKTLVLPV EQPFEKRSFK EALASPNRFI GLIAEVKKAS PSKGLIKEDF
VPVQIAKDYE AAKADAISVL TDTPFFQGEN SYLSDVKRAV SIPVLRKDFI DSLQVEESRR
IGADAILLIG EVLDPLHLHE LYLEAGEKGM DVLVEVHDAS TLEQILKVFT PDILGVNNRN
LKTFETSVKQ TEQIASLVPK ESLLVSESGI GSLEHLTFVN EHGARAVLIG ESLMRQTSQR
KAIHALFRE
