C12A5_DROME
ID C12A5_DROME Reviewed; 536 AA.
AC Q9VE01; Q6SEI2; Q8MQI5;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Probable cytochrome P450 12a5, mitochondrial;
DE EC=1.14.-.-;
DE AltName: Full=CYPXIIA5;
DE Flags: Precursor;
GN Name=Cyp12a5; ORFNames=CG11821;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=15663776; DOI=10.1111/j.1365-2583.2005.00532.x;
RA Festucci-Buselli R.A., Carvalho-Dias A.S., de Oliveira-Andrade M.,
RA Caixeta-Nunes C., Li H.-M., Stuart J.J., Muir W., Scharf M.E.,
RA Pittendrigh B.R.;
RT "Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains
RT of Drosophila melanogaster.";
RL Insect Mol. Biol. 14:69-77(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY459354; AAR88135.1; -; mRNA.
DR EMBL; AE014297; AAF55635.1; -; Genomic_DNA.
DR EMBL; AY129460; AAM76202.1; -; mRNA.
DR RefSeq; NP_650782.1; NM_142525.4.
DR AlphaFoldDB; Q9VE01; -.
DR SMR; Q9VE01; -.
DR BioGRID; 67291; 2.
DR DIP; DIP-20196N; -.
DR IntAct; Q9VE01; 3.
DR STRING; 7227.FBpp0083144; -.
DR PaxDb; Q9VE01; -.
DR PRIDE; Q9VE01; -.
DR EnsemblMetazoa; FBtr0083730; FBpp0083144; FBgn0038680.
DR GeneID; 42293; -.
DR KEGG; dme:Dmel_CG11821; -.
DR UCSC; CG11821-RA; d. melanogaster.
DR CTD; 42293; -.
DR FlyBase; FBgn0038680; Cyp12a5.
DR VEuPathDB; VectorBase:FBgn0038680; -.
DR eggNOG; KOG0159; Eukaryota.
DR GeneTree; ENSGT00940000165868; -.
DR HOGENOM; CLU_001570_28_0_1; -.
DR InParanoid; Q9VE01; -.
DR OMA; VSMIPIN; -.
DR OrthoDB; 574756at2759; -.
DR PhylomeDB; Q9VE01; -.
DR BioGRID-ORCS; 42293; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 42293; -.
DR PRO; PR:Q9VE01; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038680; Expressed in adult midgut (Drosophila) and 21 other tissues.
DR Genevisible; Q9VE01; DM.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Mitochondrion; Monooxygenase;
KW Oxidoreductase; Reference proteome; Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..536
FT /note="Probable cytochrome P450 12a5, mitochondrial"
FT /id="PRO_0000003609"
FT BINDING 482
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 66
FT /note="G -> S (in Ref. 4; AAM76202)"
FT /evidence="ECO:0000305"
FT CONFLICT 72
FT /note="L -> M (in Ref. 1; AAR88135)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 536 AA; 61355 MW; 231BFD7E6284A1F6 CRC64;
MLKGRIALNI LQSQKPIVFS ASQQRWQTNV PTAEIRNDPE WLQAKPFEEI PKANILSLFA
KSALPGGKYK NLEMMEMIDA LRQDYGNIIF LPGMMGRDGL VMTHNPKDFE VVFRNEGVWP
FRPGSDILRY HRTVYRKDFF DGVQGIIPSQ GKSWGDFRSI VNPVLMQPKN VRLYFKKMSQ
VNQEFVELIK EIRDASTQEV PGNFLETINR WTLESVSVVA LDKQLGLLRE SGKNSEATKL
FKYLDEFFLH SADLEMKPSL WRYFKTPLLK KMLRTMDSVQ EVTLKYVDEA IERLEKEAKE
GVVRPEHEQS VLEKLLKVDK KVATVMAMDM LMAGVDTTSS TFTALLLCLA KNPEKQARLR
EEVMKVLPNK DSEFTEASMK NVPYLRACIK ESQRVYPLVI GNARGLTRDS VISGYRVPAG
TIVSMIPINS LYSEEYFPKP TEFLPERWLR NASDSAGKCP ANDLKTKNPF VFLPFGFGPR
MCVGKRIVEM ELELGTARLI RNFNVEFNHS TKNAFRSALI NLPNIPLKFK FTDVPN
