ID   C12AA_BACTU             Reviewed;        1257 AA.
AC   Q45754;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Pesticidal crystal protein Cry12Aa;
DE   AltName: Full=142 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryXIIA(a);
GN   Name=cry12Aa; Synonyms=cryVB, cryXIIA(a);
OS   Bacillus thuringiensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-11.
RC   STRAIN=NRRL B-18244 / PS33F2;
RA   Narva K.E., Payne J.M., Schwab G.E., Hickle L.A., Galasan T., Sick A.J.;
RT   "Novel Bacillus thuringiensis microbes active against nematodes, and genes
RT   encoding novel nematode-active toxins cloned from Bacillus thuringi.";
RL   Patent number EP0462721, 27-DEC-1991.
CC   -!- FUNCTION: Endotoxin with nematicidal activity.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; L07027; AAA22355.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q45754; -.
DR   SMR; Q45754; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Sporulation; Toxin; Virulence.
FT   CHAIN           1..1257
FT                   /note="Pesticidal crystal protein Cry12Aa"
FT                   /id="PRO_0000174085"
SQ   SEQUENCE   1257 AA;  142266 MW;  3D988FFC6C0E3981 CRC64;
     MATLNEVYPV NYNVLSSDAF QQLDTTGFKS KYDEMIKAFE KKWKKGAKGK DLLDVAWTYI
     TTGEIDPLNV IKGVLSVLTL IPEVGTVASA ASTIVSFIWP KIFGDKPNAK NIFEELKPQI
     EALIQQDITN YQDAINQKKF DSLQKTINLY TVAIDNNDYV TAKTQLENLN SILTSDISIF
     IPEGYETGGL PYYAMVANAH ILLLRDAIVN AEKLGFSDKE VDTHKKYIKM TIHNHTEAVI
     KAFLNGLDKF KSLDVNSYNK KANYIKGMTE MVLDLVALWP TFDPDHYQKE VEIEFTRTIS
     SPIYQPVPKN MQNTSSSIVP SDLFHYQGDL VKLEFSTRTD NDGLAKIFTG IRNTFYKSPN
     THETYHVDFS YNTQSSGNIS RGSSNPIPID LNNPIISTCI RNSFYKAIAG SSVLVNFKDG
     TQGYAFAQAP TGGAWDHSFI ESDGAPEGHK LNYIYTSPGD TLRDFINVYT LISTPTINEL
     STEKIKGFPA EKGYIKNQGI MKYYGKPEYI NGAQPVNLEN QQTLIFEFHA SKTAQYTIRI
     RYASTQGTKG YFRLDNQELQ TLNIPTSHNG YVTGNIGENY DLYTIGSYTI TEGNHTLQIQ
     HNDKNGMVLD RIEFVPKDSL QDSPQDSPPE VHESTIIFDK SSPTIWSSNK HSYSHIHLEG
     SYTSQGSYPH NLLINLFHPT DPNRNHTIHV NNGDMNVDYG KDSVADGLNF NKITATIPSD
     AWYSGTITSM HLFNDNNFKT ITPKFELSNE LENITTQVNA LFASSAQDTL ASNVSDYWIE
     QVVMKVDALS DEVFGKEKKA LRKLVNQAKR LSKIRNLLIG GNFDNLVAWY MGKDVVKESD
     HELFKSDHVL LPPPTFHPSY IFQKVEESKL KPNTRYTISG FIAHGEDVEL VVSRYGQEIQ
     KVMQVPYEEA LPLTSESNSS CCVPNLNINE TLADPHFFSY SIDVGSLEME ANPGIEFGLR
     IVKPTGMARV SNLEIREDRP LTAKEIRQVQ RAARDWKQNY EQERTEITAI IQPVLNQINA
     LYENEDWNGS IRSNVSYHDL EQIMLPTLLK TEEINCNYDH PAFLLKVYHW FMTDRIGEHG
     TILARFQEAL DRAYTQLESR NLLHNGHFTT DTANWTIEGD AHHTILEDGR RVLRLPDWSS
     NATQTIEIED FDLDQEYQLL IHAKGKGSIT LQHGEENEYV ETHTHHTNDF ITSQNIPFTF
     KGNQIEVHIT SEDGEFLIDH ITVIEVSKTD TNTNIIENSP INTSMNSNVR VDIPRSL