TRPC_MYCTU
ID TRPC_MYCTU Reviewed; 272 AA.
AC P9WFX7; L0T8S6; O06129; P0A632;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 39.
DE RecName: Full=Indole-3-glycerol phosphate synthase;
DE Short=IGPS;
DE EC=4.1.1.48;
GN Name=trpC; OrderedLocusNames=Rv1611; ORFNames=MTCY01B2.03;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA Raman K., Yeturu K., Chandra N.;
RT "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT through an interactome, reactome and genome-scale structural analysis.";
RL BMC Syst. Biol. 2:109-109(2008).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+)
CC = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O;
CC Xref=Rhea:RHEA:23476, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:58613, ChEBI:CHEBI:58866; EC=4.1.1.48;
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 4/5.
CC -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC -!- SIMILARITY: Belongs to the TrpC family. {ECO:0000305}.
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DR EMBL; AL123456; CCP44375.1; -; Genomic_DNA.
DR PIR; A70557; A70557.
DR RefSeq; NP_216127.1; NC_000962.3.
DR RefSeq; WP_003407990.1; NZ_NVQJ01000016.1.
DR PDB; 3QJA; X-ray; 1.29 A; A=1-272.
DR PDB; 3T40; X-ray; 1.75 A; A=1-272.
DR PDB; 3T44; X-ray; 1.60 A; A=1-272.
DR PDB; 3T55; X-ray; 2.06 A; A=1-272.
DR PDB; 3T78; X-ray; 1.60 A; A=1-272.
DR PDB; 4FB7; X-ray; 1.30 A; A=1-272.
DR PDBsum; 3QJA; -.
DR PDBsum; 3T40; -.
DR PDBsum; 3T44; -.
DR PDBsum; 3T55; -.
DR PDBsum; 3T78; -.
DR PDBsum; 4FB7; -.
DR AlphaFoldDB; P9WFX7; -.
DR SMR; P9WFX7; -.
DR STRING; 83332.Rv1611; -.
DR PaxDb; P9WFX7; -.
DR DNASU; 885294; -.
DR GeneID; 885294; -.
DR KEGG; mtu:Rv1611; -.
DR TubercuList; Rv1611; -.
DR eggNOG; COG0134; Bacteria.
DR OMA; RGPHDLI; -.
DR PhylomeDB; P9WFX7; -.
DR BRENDA; 4.1.1.48; 3445.
DR SABIO-RK; P9WFX7; -.
DR UniPathway; UPA00035; UER00043.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0004425; F:indole-3-glycerol-phosphate synthase activity; IDA:MTBBASE.
DR GO; GO:0000287; F:magnesium ion binding; IDA:MTBBASE.
DR GO; GO:0004640; F:phosphoribosylanthranilate isomerase activity; IBA:GO_Central.
DR GO; GO:0046391; P:5-phosphoribose 1-diphosphate metabolic process; IDA:MTBBASE.
DR GO; GO:0000162; P:tryptophan biosynthetic process; IDA:MTBBASE.
DR CDD; cd00331; IGPS; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00134_B; IGPS_B; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR045186; Indole-3-glycerol_P_synth.
DR InterPro; IPR013798; Indole-3-glycerol_P_synth_dom.
DR InterPro; IPR001468; Indole-3-GlycerolPSynthase_CS.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR PANTHER; PTHR22854; PTHR22854; 1.
DR Pfam; PF00218; IGPS; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR PROSITE; PS00614; IGPS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Amino-acid biosynthesis; Aromatic amino acid biosynthesis;
KW Decarboxylase; Lyase; Reference proteome; Tryptophan biosynthesis.
FT CHAIN 1..272
FT /note="Indole-3-glycerol phosphate synthase"
FT /id="PRO_0000154234"
FT HELIX 5..22
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 27..36
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 43..47
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 53..58
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 60..62
FT /evidence="ECO:0007829|PDB:3T44"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:4FB7"
FT HELIX 74..83
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 87..92
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 95..97
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 98..111
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 116..120
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 125..133
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 137..142
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 148..160
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 164..171
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 172..181
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 184..191
FT /evidence="ECO:0007829|PDB:3QJA"
FT TURN 193..195
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 202..206
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 207..209
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 214..220
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 225..233
FT /evidence="ECO:0007829|PDB:3QJA"
FT STRAND 237..241
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 243..246
FT /evidence="ECO:0007829|PDB:3QJA"
FT HELIX 251..259
FT /evidence="ECO:0007829|PDB:3QJA"
FT TURN 260..263
FT /evidence="ECO:0007829|PDB:3QJA"
SQ SEQUENCE 272 AA; 28023 MW; 9CA29D0F0FAC76C2 CRC64;
MSPATVLDSI LEGVRADVAA REASVSLSEI KAAAAAAPPP LDVMAALREP GIGVIAEVKR
ASPSAGALAT IADPAKLAQA YQDGGARIVS VVTEQRRFQG SLDDLDAVRA SVSIPVLRKD
FVVQPYQIHE ARAHGADMLL LIVAALEQSV LVSMLDRTES LGMTALVEVH TEQEADRALK
AGAKVIGVNA RDLMTLDVDR DCFARIAPGL PSSVIRIAES GVRGTADLLA YAGAGADAVL
VGEGLVTSGD PRAAVADLVT AGTHPSCPKP AR
