ID   C14AA_BACTS             Reviewed;        1186 AA.
AC   Q45710;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Pesticidal crystal protein Cry14Aa;
DE   AltName: Full=132 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryXIVA(a);
GN   Name=cry14Aa; Synonyms=cryXIVA(a);
OS   Bacillus thuringiensis subsp. sotto.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=29340;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18679 / PS80JJ1;
RA   Payne J.M., Narva K.E.;
RT   "Novel Bacillus thuringiensis toxins active against corn rootworm larvae.";
RL   Patent number WO9416079, 21-JUL-1994.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of insects.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; U13955; AAA21516.1; -; Genomic_DNA.
DR   PIR; T18210; T18210.
DR   AlphaFoldDB; Q45710; -.
DR   SMR; Q45710; -.
DR   PRIDE; Q45710; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1186
FT                   /note="Pesticidal crystal protein Cry14Aa"
FT                   /id="PRO_0000174087"
SQ   SEQUENCE   1186 AA;  131695 MW;  EF4BB1BAE2CB9487 CRC64;
     MDCNLQSQQN IPYNVLAIPV SNVNALVDTA GDLKKAWEEF QKTGSFSLTA LQQGFSASQG
     GAFNYLTLLQ SGISLAGSFV PGGTFVAPIV NMVIGWLWPH KNKTADTENL IKLIDEEIQK
     QLNKALLDQD RNNWTSFLES IFDTSATVSN AIIDAQWSGT VDTTNRQQKT PTTSDYLNVV
     GKFDSADSSI ITNENQIMNG NFDVAAAPYF VIGATLRLSL YQSYIKFCNS WIDAVGFSTN
     DANTQKANLA RTKLTMRTTI NEYTQRVMKV FKDSKNMPTI GTNKFSVDAY NVYVKGMTLN
     VLDMVAIWSS LYPNDYTSQT AIEQTRVTFS NMVGQEEGTD GTLKIYNTFD SLSYQHSLIP
     NNNVNLISYY TDELQNLELA VYTPKGGSGY AYPYGFILNY ANSNYKYGDN DPTGKPLNKQ
     DGPIQQINAA TQNSKYLDGE TINGIGASLP GYCTTGCSAT EQPFSCTSTA NSYKASCNPS
     DTNQKINALY AFTQTNVKGS TGKLGVLASL VPYDLNPKNV FGELDSDTNN VILKGIPAEK
     GYFPNNARPT VVKEWINGAS AVPFYSGNTL FMTATNLTAT QYKIRIRYAN PNSDTQIGVL
     ITQNGSQISN SNLTLYSTTD SSMSSNLPQN VYVTGENGNY TLLDLYSTTN VLSTGDITLK
     LTGGNQKIFI DRIEFIPTMP VPAPTNNTNN NNGDNGNNNP PHHGCAIAGT QQLCSGPPKF
     EQVSDLEKIT TQVYMLFKSS SYEELALKVS SYQINQVALK VMALSDEKFC EEKRLLRKLV
     NKANQLLEAR NLLVGGNFET TQNWVLGTNA YINYDSFLFN GNYLSLQPAS GFFTSYAYQK
     IDESTLKPYT RYKVSGFIGQ SNQVELIISR YGKEIDKILN VPYAGPLPIT ADASITCCAP
     EIDQCDGGQS DSHFFNYSID VGALHPELNP GIEIGLKIVQ SNGYITISNL EIIEERPLTE
     MEIQAVNRKD QKWKREKLLE CASVSELLQP IINQIDSLFK DANWYNDILP HVTYQTLKNI
     IVPDLPKLKH WFIDHLPGEY HEIEQKMKEA LKHAFTQLDE KNLIHNGHFA TNLIDWQVEG
     DARMKVLENN ALALQLSNWD SSVSQSIDIL EFDEDKAYKL RVYAQGSGTI QFGNCEDEAI
     QFNTNSFVYK EKIIYFDTPS INLHIQSEGS EFVVSSIDLV ELSDDE