ID   C14B2_ORYSJ             Reviewed;         532 AA.
AC   Q0DS59; A0A0P0VX58; B9F8A0; B9F8A1; Q10LX1;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 2.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Cytochrome P450 714B2;
DE            EC=1.14.-.-;
DE   AltName: Full=GA 13-oxidase 2;
DE   AltName: Full=Gibberellin 13 oxidase 2;
GN   Name=CYP714B2;
GN   OrderedLocusNames=Os03g0332100, LOC_Os03g21400, LOC_Os03g21419;
GN   ORFNames=OsJ_10714, OsJ_10715;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   FUNCTION, TISSUE SPECIFICITY, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, AND
RP   INDUCTION BY GIBBERELLIN.
RX   PubMed=23319637; DOI=10.1073/pnas.1215788110;
RA   Magome H., Nomura T., Hanada A., Takeda-Kamiya N., Ohnishi T., Shinma Y.,
RA   Katsumata T., Kawaide H., Kamiya Y., Yamaguchi S.;
RT   "CYP714B1 and CYP714B2 encode gibberellin 13-oxidases that reduce
RT   gibberellin activity in rice.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:1947-1952(2013).
CC   -!- FUNCTION: Catalyzes the 13-hydroxylation of gibberellins (GAs).
CC       Determines the ratio of GA4 and GA1. Converts GA12 into GA53.
CC       {ECO:0000269|PubMed:23319637}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type III
CC       membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in shoot, spikelet and uppermost
CC       internode. Detected in roots, leaves and anthers.
CC       {ECO:0000269|PubMed:23319637}.
CC   -!- INDUCTION: Up-regulated by bioactive gibberellins.
CC       {ECO:0000269|PubMed:23319637}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype and no change in the levels
CC       of 13-OH GAs; due to the redundancy with CYP714B1. Cyp714b1 and
CC       cyp714b2 double mutants have decreased levels of 13-OH GAs, increased
CC       levels of 13-H GAs, including GA4, and longer uppermost internode.
CC       {ECO:0000269|PubMed:23319637}.
CC   -!- MISCELLANEOUS: Overexpression of CYP714B2 in a heterologous system
CC       causes semi-dwarfism and increased 13-OH GAs content.
CC       {ECO:0000305|PubMed:23319637}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABF95769.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAF11929.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=EEE58998.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=EEE58999.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; DP000009; ABF95769.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008209; BAF11929.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014959; BAS84037.1; -; Genomic_DNA.
DR   EMBL; CM000140; EEE58998.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CM000140; EEE58999.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_015628304.1; XM_015772818.1.
DR   AlphaFoldDB; Q0DS59; -.
DR   SMR; Q0DS59; -.
DR   STRING; 4530.OS03T0332100-00; -.
DR   PaxDb; Q0DS59; -.
DR   PRIDE; Q0DS59; -.
DR   EnsemblPlants; Os03t0332100-00; Os03t0332100-00; Os03g0332100.
DR   GeneID; 4332734; -.
DR   Gramene; Os03t0332100-00; Os03t0332100-00; Os03g0332100.
DR   KEGG; osa:4332734; -.
DR   eggNOG; KOG0157; Eukaryota.
DR   HOGENOM; CLU_001570_5_0_1; -.
DR   InParanoid; Q0DS59; -.
DR   OMA; MVPYTYL; -.
DR   OrthoDB; 825914at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q0DS59; OS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   1: Evidence at protein level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT   CHAIN           1..532
FT                   /note="Cytochrome P450 714B2"
FT                   /id="PRO_0000422413"
FT   TOPO_DOM        1..2
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        3..23
FT                   /note="Helical; Signal-anchor for type III membrane
FT                   protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        24..532
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   BINDING         474
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   532 AA;  59524 MW;  BEB846B728846221 CRC64;
     MEVGMVVVVA AKVLVSLWCV GACCLAAYLY RVVWVAPRRV LAEFRRQGIG GPRPSFPYGN
     LADMREAVAA ARHQLAEARR RRRARDSGDG GSGAGIVHDY RPAVLPFYEK WRKDYGPIFT
     YSMGNVVFLH VSRPDVVRDI NLCVSLDLGK SSYLKATHEP LFGGGILKSN GEAWAHQRKI
     IAREFFLDKV KGMVDLMVDS AQTLLKSWEE GIDKNGGTID IKIDDDIRAY SADVISRTCF
     GSSYIKGKNI FLKIRELQKA VSKPNVLAEM TGLRFFPIKR NKQAWELHKQ VHKLILEIVK
     ESGEERNLLR AILLSASSSK VELAEAENFI VDNCKSIYFA GYESTAVTAA WCLMLLGLHP
     EWQDRVREEV QEVCAGQPVD SQSLQKMKNL TMVIQETLRL YPAGAFVSRQ ALQELKFGGV
     HIPKGVNIYI PVSTMHLDPN LWGPDVKEFN PERFSNAQPQ LHSYLPFGAG ARTCLGQGFA
     MAELKTLISL IISKFVLKLS PNYEHSPTLK LIVEPEFGVD LSLTRVQGAY RH