ACAP3_HUMAN
ID ACAP3_HUMAN Reviewed; 834 AA.
AC Q96P50; B1AMF5; Q5TA42; Q5TA43; Q86UT3; Q9BSR9; Q9C0E7;
DT 27-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 3;
DE AltName: Full=Centaurin-beta-5;
DE Short=Cnt-b5;
GN Name=ACAP3; Synonyms=CENTB5, KIAA1716;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Hong W.;
RT "Centaurin beta5, a new member of centaurin family.";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=11214970; DOI=10.1093/dnares/7.6.347;
RA Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XIX. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:347-355(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Hippocampus, and Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=16964243; DOI=10.1038/nbt1240;
RA Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
RT "A probability-based approach for high-throughput protein phosphorylation
RT analysis and site localization.";
RL Nat. Biotechnol. 24:1285-1292(2006).
CC -!- FUNCTION: GTPase-activating protein for the ADP ribosylation factor
CC family. {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q96P50-3; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96P50-1; Sequence=VSP_035993, VSP_035994;
CC Name=3;
CC IsoId=Q96P50-4; Sequence=VSP_035992;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB21807.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF411981; AAL04165.1; -; mRNA.
DR EMBL; AB051503; BAB21807.1; ALT_INIT; mRNA.
DR EMBL; AL139287; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL162741; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471183; EAW56248.1; -; Genomic_DNA.
DR EMBL; CH471183; EAW56249.1; -; Genomic_DNA.
DR EMBL; BC004874; AAH04874.2; -; mRNA.
DR EMBL; BC051194; AAH51194.2; -; mRNA.
DR CCDS; CCDS19.2; -. [Q96P50-3]
DR RefSeq; NP_085152.2; NM_030649.2. [Q96P50-3]
DR AlphaFoldDB; Q96P50; -.
DR SMR; Q96P50; -.
DR BioGRID; 125546; 21.
DR IntAct; Q96P50; 4.
DR MINT; Q96P50; -.
DR STRING; 9606.ENSP00000346733; -.
DR iPTMnet; Q96P50; -.
DR PhosphoSitePlus; Q96P50; -.
DR BioMuta; ACAP3; -.
DR DMDM; 218511905; -.
DR EPD; Q96P50; -.
DR jPOST; Q96P50; -.
DR MassIVE; Q96P50; -.
DR MaxQB; Q96P50; -.
DR PaxDb; Q96P50; -.
DR PeptideAtlas; Q96P50; -.
DR PRIDE; Q96P50; -.
DR ProteomicsDB; 77629; -. [Q96P50-3]
DR ProteomicsDB; 77630; -. [Q96P50-1]
DR ProteomicsDB; 77631; -. [Q96P50-4]
DR Antibodypedia; 46438; 123 antibodies from 21 providers.
DR DNASU; 116983; -.
DR Ensembl; ENST00000353662.4; ENSP00000321139.4; ENSG00000131584.19. [Q96P50-1]
DR Ensembl; ENST00000354700.10; ENSP00000346733.5; ENSG00000131584.19. [Q96P50-3]
DR GeneID; 116983; -.
DR KEGG; hsa:116983; -.
DR MANE-Select; ENST00000354700.10; ENSP00000346733.5; NM_030649.3; NP_085152.2.
DR UCSC; uc001aeb.3; human. [Q96P50-3]
DR CTD; 116983; -.
DR GeneCards; ACAP3; -.
DR HGNC; HGNC:16754; ACAP3.
DR HPA; ENSG00000131584; Low tissue specificity.
DR neXtProt; NX_Q96P50; -.
DR OpenTargets; ENSG00000131584; -.
DR PharmGKB; PA26408; -.
DR VEuPathDB; HostDB:ENSG00000131584; -.
DR eggNOG; KOG0521; Eukaryota.
DR GeneTree; ENSGT00940000156199; -.
DR HOGENOM; CLU_012513_0_1_1; -.
DR InParanoid; Q96P50; -.
DR OMA; SHYHGII; -.
DR OrthoDB; 751525at2759; -.
DR PhylomeDB; Q96P50; -.
DR TreeFam; TF318315; -.
DR PathwayCommons; Q96P50; -.
DR SignaLink; Q96P50; -.
DR BioGRID-ORCS; 116983; 22 hits in 1074 CRISPR screens.
DR GenomeRNAi; 116983; -.
DR Pharos; Q96P50; Tbio.
DR PRO; PR:Q96P50; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q96P50; protein.
DR Bgee; ENSG00000131584; Expressed in right hemisphere of cerebellum and 152 other tissues.
DR ExpressionAtlas; Q96P50; baseline and differential.
DR Genevisible; Q96P50; HS.
DR GO; GO:0030426; C:growth cone; IEA:Ensembl.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0001764; P:neuron migration; IEA:Ensembl.
DR GO; GO:0010975; P:regulation of neuron projection development; IEA:Ensembl.
DR CDD; cd07637; BAR_ACAP3; 1.
DR Gene3D; 1.10.220.150; -; 1.
DR Gene3D; 1.20.1270.60; -; 1.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR045258; ACAP1/2/3-like.
DR InterPro; IPR042695; ACAP3_BAR.
DR InterPro; IPR027267; AH/BAR_dom_sf.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR23180; PTHR23180; 1.
DR Pfam; PF13857; Ank_5; 1.
DR Pfam; PF01412; ArfGap; 1.
DR Pfam; PF00169; PH; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00105; ArfGap; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF103657; SSF103657; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
DR PROSITE; PS50115; ARFGAP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ANK repeat; GTPase activation; Metal-binding;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..834
FT /note="Arf-GAP with coiled-coil, ANK repeat and PH domain-
FT containing protein 3"
FT /id="PRO_0000356159"
FT DOMAIN 268..363
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 403..525
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REPEAT 702..731
FT /note="ANK 1"
FT REPEAT 735..764
FT /note="ANK 2"
FT REPEAT 768..797
FT /note="ANK 3"
FT ZN_FING 418..441
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT REGION 375..400
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 633..653
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 376..393
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 636..651
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..270
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_035992"
FT VAR_SEQ 1..42
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11214970, ECO:0000303|Ref.1"
FT /id="VSP_035993"
FT VAR_SEQ 605..638
FT /note="SLSSDSGLGGSSDGSSDVLAFGSGSVVDSVTEEE -> K (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:11214970, ECO:0000303|Ref.1"
FT /id="VSP_035994"
FT CONFLICT 94
FT /note="I -> N (in Ref. 5; AAH51194)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 834 AA; 92495 MW; D4C00A06EDB72F68 CRC64;
MTVEFEECVK DSPRFRATID EVETDVVEIE AKLDKLVKLC SGMVEAGKAY VSTSRLFVSG
VRDLSQQCQG DTVISECLQR FADSLQEVVN YHMILFDQAQ RSVRQQLQSF VKEDVRKFKE
TKKQFDKVRE DLELSLVRNA QAPRHRPHEV EEATGALTLT RKCFRHLALD YVLQINVLQA
KKKFEILDSM LSFMHAQSSF FQQGYSLLHQ LDPYMKKLAA ELDQLVIDSA VEKREMERKH
AAIQQRTLLQ DFSYDESKVE FDVDAPSGVV MEGYLFKRAS NAFKTWNRRW FSIQNSQLVY
QKKLKDALTV VVDDLRLCSV KPCEDIERRF CFEVLSPTKS CMLQADSEKL RQAWVQAVQA
SIASAYRESP DSCYSERLDR TASPSTSSID SATDTRERGV KGESVLQRVQ SVAGNSQCGD
CGQPDPRWAS INLGVLLCIE CSGIHRSLGV HCSKVRSLTL DSWEPELLKL MCELGNSAVN
QIYEAQCEGA GSRKPTASSS RQDKEAWIKD KYVEKKFLRK APMAPALEAP RRWRVQKCLR
PHSSPRAPTA RRKVRLEPVL PCVAALSSVG TLDRKFRRDS LFCPDELDSL FSYFDAGAAG
AGPRSLSSDS GLGGSSDGSS DVLAFGSGSV VDSVTEEEGA ESEESSGEAD GDTEAEAWGL
ADVRELHPGL LAHRAARARD LPALAAALAH GAEVNWADAE DEGKTPLVQA VLGGSLIVCE
FLLQNGADVN QRDSRGRAPL HHATLLGRTG QVCLFLKRGA DQHALDQEQR DPLAIAVQAA
NADIVTLLRL ARMAEEMREA EAAPGPPGAL AGSPTELQFR RCIQEFISLH LEES
